(Linear) (Six-base) MAPSORT of: KIEE4477.seq Check: 7762 from: 1 to: 1485 KIEE4477.orf With 182 enzymes: SgfI * March 22, 2012 18:09 .. AccI GT'mk_AC Cuts at: 0 200 1485 Size: 200 1285 AfeI AGC'GCT Cuts at: 0 934 1485 Size: 934 551 AflIII A'CryG_T Cuts at: 0 1289 1485 Size: 1289 196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1252 1485 Size: 1252 233 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1399 1431 1485 Size: 1399 32 54 Fragments arranged by size: 1399 54 32 AlwNI CAG_nnn'CTG Cuts at: 0 705 1344 1485 Size: 705 639 141 Fragments arranged by size: 705 639 141 ApaI G_GGCC'C Cuts at: 0 108 116 1424 1485 Size: 108 8 1308 61 Fragments arranged by size: 1308 108 61 8 ApoI r'AATT_y Cuts at: 0 96 1485 Size: 96 1389 AscI GG'CGCG_CC Cuts at: 0 727 1485 Size: 727 758 AvaI C'yCGr_G Cuts at: 0 101 107 190 1024 1324 1423 1485 Size: 101 6 83 834 300 99 62 Fragments arranged by size: 834 300 101 99 83 62 6 AvrII C'CTAG_G Cuts at: 0 91 1485 Size: 91 1394 BanI G'GyrC_C Cuts at: 0 186 408 500 633 655 1347 1380 1485 Size: 186 222 92 133 22 692 33 105 Fragments arranged by size: 692 222 186 133 105 92 33 22 BanII G_rGCy'C Cuts at: 0 108 116 1412 1424 1485 Size: 108 8 1296 12 61 Fragments arranged by size: 1296 108 61 12 8 BbeI G_GCGC'C Cuts at: 0 637 1384 1485 Size: 637 747 101 Fragments arranged by size: 747 637 101 BbvCI CC'TCA_GC Cuts at: 0 404 1485 Size: 404 1081 BglI GCCn_nnn'nGGC Cuts at: 0 31 1485 Size: 31 1454 BlpI GC'TnA_GC Cuts at: 0 770 1485 Size: 770 715 Bme1580I G_kGCm'C Cuts at: 0 108 116 191 658 1352 1424 1485 Size: 108 8 75 467 694 72 61 Fragments arranged by size: 694 467 108 75 72 61 8 BmgBI CAC'GTC Cuts at: 0 460 1086 1113 1485 Size: 460 626 27 372 Fragments arranged by size: 626 460 372 27 BmrI ACTGGGnnnn_n' Cuts at: 0 1437 1485 Size: 1437 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1050 1167 1485 Size: 1050 117 318 Fragments arranged by size: 1050 318 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 442 871 1018 1426 1485 Size: 295 147 429 147 408 59 Fragments arranged by size: 429 408 295 147 147 59 Bpu10I CC'TnA_GC Cuts at: 0 404 1485 Size: 404 1081 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1396 1485 Size: 1396 89 BsaI GGTCTCn'nnnn_ Cuts at: 0 346 868 964 1485 Size: 346 522 96 521 Fragments arranged by size: 522 521 346 96 BsaAI yAC'GTr Cuts at: 0 360 1242 1292 1485 Size: 360 882 50 193 Fragments arranged by size: 882 360 193 50 BsaHI Gr'CG_yC Cuts at: 0 575 634 940 1274 1381 1485 Size: 575 59 306 334 107 104 Fragments arranged by size: 575 334 306 107 104 59 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 578 608 1399 1429 1485 Size: 578 30 791 30 56 Fragments arranged by size: 791 578 56 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 943 1485 Size: 943 542 BseYI C'CCAG_C Cuts at: 0 222 227 689 813 833 1041 1485 Size: 222 5 462 124 20 208 444 Fragments arranged by size: 462 444 222 208 124 20 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1234 1485 Size: 718 516 251 Fragments arranged by size: 718 516 251 BsiEI CG_ry'CG Cuts at: 0 35 649 1193 1485 Size: 35 614 544 292 Fragments arranged by size: 614 544 292 35 BsiHKAI G_wGCw'C Cuts at: 0 602 1331 1412 1485 Size: 602 729 81 73 Fragments arranged by size: 729 602 81 73 Bsp1286I G_dGCh'C Cuts at: 0 108 116 191 602 658 1331 1352 1412 Size: 108 8 75 411 56 673 21 60 Cuts at: 1412 1424 1485 Size: 12 61 Fragments arranged by size: 673 411 108 75 61 60 56 21 12 8 BspHI T'CATG_A Cuts at: 0 1277 1485 Size: 1277 208 BsrDI GCAATG_nn' Cuts at: 0 1275 1485 Size: 1275 210 BsrFI r'CCGG_y Cuts at: 0 133 326 648 1147 1304 1363 1485 Size: 133 193 322 499 157 59 122 Fragments arranged by size: 499 322 193 157 133 122 59 BssHII G'CGCG_C Cuts at: 0 606 727 757 809 957 959 1485 Size: 606 121 30 52 148 2 526 Fragments arranged by size: 606 526 148 121 52 30 2 BssSI C'ACGA_G Cuts at: 0 72 1485 Size: 72 1413 BstAPI GCAn_nnn'nTGC Cuts at: 0 1335 1485 Size: 1335 150 BstXI CCAn_nnnn'nTGG Cuts at: 0 504 1485 Size: 504 981 BtgI C'CryG_G Cuts at: 0 233 482 722 746 896 1056 1072 1485 Size: 233 249 240 24 150 160 16 413 Fragments arranged by size: 413 249 240 233 160 150 24 16 BtsI GCAGTG_nn' Cuts at: 0 1036 1459 1485 Size: 1036 423 26 Fragments arranged by size: 1036 423 26 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 354 389 1485 Size: 354 35 1096 Fragments arranged by size: 1096 354 35 EaeI y'GGCC_r Cuts at: 0 32 328 362 467 485 646 743 1485 Size: 32 296 34 105 18 161 97 742 Fragments arranged by size: 742 296 161 105 97 34 32 18 EagI C'GGCC_G Cuts at: 0 32 646 1485 Size: 32 614 839 Fragments arranged by size: 839 614 32 EarI CTCTTCn'nnn_ Cuts at: 0 67 625 1485 Size: 67 558 860 Fragments arranged by size: 860 558 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 811 1485 Size: 811 674 EcoICRI GAG'CTC Cuts at: 0 1410 1485 Size: 1410 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 442 871 1018 1426 1485 Size: 295 147 429 147 408 59 Fragments arranged by size: 429 408 295 147 147 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 925 1485 Size: 925 560 EcoO109I rG'GnC_Cy Cuts at: 0 112 113 1485 Size: 112 1 1372 Fragments arranged by size: 1372 112 1 EcoRI G'AATT_C Cuts at: 0 96 1485 Size: 96 1389 HaeII r_GCGC'y Cuts at: 0 307 442 637 802 936 1384 1485 Size: 307 135 195 165 134 448 101 Fragments arranged by size: 448 307 195 165 135 134 101 KasI G'GCGC_C Cuts at: 0 633 1380 1485 Size: 633 747 105 Fragments arranged by size: 747 633 105 MscI TGG'CCA Cuts at: 0 364 745 1485 Size: 364 381 740 Fragments arranged by size: 740 381 364 MspA1I CmG'CkG Cuts at: 0 14 569 702 724 741 1131 1344 1485 Size: 14 555 133 22 17 390 213 141 Fragments arranged by size: 555 390 213 141 133 22 17 14 NaeI GCC'GGC Cuts at: 0 135 1149 1365 1485 Size: 135 1014 216 120 Fragments arranged by size: 1014 216 135 120 NarI GG'CG_CC Cuts at: 0 634 1381 1485 Size: 634 747 104 Fragments arranged by size: 747 634 104 NcoI C'CATG_G Cuts at: 0 1072 1485 Size: 1072 413 NgoMIV G'CCGG_C Cuts at: 0 133 1147 1363 1485 Size: 133 1014 216 122 Fragments arranged by size: 1014 216 133 122 NotI GC'GGCC_GC Cuts at: 0 32 1485 Size: 32 1453 PflMI CCAn_nnn'nTGG Cuts at: 0 234 1485 Size: 234 1251 PfoI T'CCnGG_A Cuts at: 0 1403 1485 Size: 1403 82 PmlI CAC'GTG Cuts at: 0 1242 1292 1485 Size: 1242 50 193 Fragments arranged by size: 1242 193 50 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1399 1431 1485 Size: 1399 32 54 Fragments arranged by size: 1399 54 32 PspOMI G'GGCC_C Cuts at: 0 104 112 1420 1485 Size: 104 8 1308 65 Fragments arranged by size: 1308 104 65 8 PspXI vC'TCGA_Gb Cuts at: 0 1024 1485 Size: 1024 461 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1429 1461 1485 Size: 1429 32 24 Fragments arranged by size: 1429 32 24 PstI C_TGCA'G Cuts at: 0 83 215 674 1340 1485 Size: 83 132 459 666 145 Fragments arranged by size: 666 459 145 132 83 PvuII CAG'CTG Cuts at: 0 702 1131 1485 Size: 702 429 354 Fragments arranged by size: 702 429 354 SacI G_AGCT'C Cuts at: 0 1412 1485 Size: 1412 73 SacII CC_GC'GG Cuts at: 0 725 1485 Size: 725 760 SfcI C'TryA_G Cuts at: 0 79 211 670 1336 1485 Size: 79 132 459 666 149 Fragments arranged by size: 666 459 149 132 79 SfoI GGC'GCC Cuts at: 0 635 1382 1485 Size: 635 747 103 Fragments arranged by size: 747 635 103 SmaI CCC'GGG Cuts at: 0 103 109 1425 1485 Size: 103 6 1316 60 Fragments arranged by size: 1316 103 60 6 SmlI C'TyrA_G Cuts at: 0 1024 1411 1485 Size: 1024 387 74 Fragments arranged by size: 1024 387 74 StyI C'CwwG_G Cuts at: 0 91 1072 1283 1485 Size: 91 981 211 202 Fragments arranged by size: 981 211 202 91 TatI w'GTAC_w Cuts at: 0 1217 1485 Size: 1217 268 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 578 610 884 916 1485 Size: 578 32 274 32 569 Fragments arranged by size: 578 569 274 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 297 372 1485 Size: 297 75 1113 Fragments arranged by size: 1113 297 75 XhoI C'TCGA_G Cuts at: 0 1024 1485 Size: 1024 461 XmaI C'CCGG_G Cuts at: 0 101 107 1423 1485 Size: 101 6 1316 62 Fragments arranged by size: 1316 101 62 6 Enzymes that do cut: AccI AfeI AflIII AlfI AloI AlwNI ApaI ApoI AscI AvaI AvrII BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BssHII BssSI BstAPI BstXI BtgI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII KasI MscI MspA1I NaeI NarI NcoI NgoMIV NotI PflMI PfoI PmlI PpiI PspOMI PspXI PsrI PstI PvuII SacI SacII SfcI SfoI SmaI SmlI StyI TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AhdI AleI ApaLI AseI AsiSI BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BmtI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MslI NdeI NheI NruI NsiI NspI PacI PasI PciI PmeI PpuMI PshAI PsiI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XmnI ZraI