################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI Bst1107I BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvaI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 43 CGCG CG^CG AcsI 112 AAATTC [GA]^AATT[CT] ApoI 112 AAATTC [GA]^AATT[CT] AvaII 279 GGACC G^G[AT]CC BbrPI 49 CACGTG CAC^GTG BbsI 172 GTCTTC GAAGAC(2/6) BcgI 54 GCAGCCTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 172 GTCTTC GAAGAC(2/6) BsaAI 49 CACGTG [CT]AC^GT[GA] BspDI 257 ATCGAT AT^CGAT BspMI 306 ACCTGC ACCTGC(4/8) BssSI 263 CTCGTG CACGAG(-5/-1) BstBI 122 TTCGAA TT^CGAA BstUI 43 CGCG CG^CG ClaI 257 ATCGAT AT^CGAT DpnII 260 GATC ^GATC DsaI 42 CCGCGG C^C[GA][CT]GG EaeI 45 CGGCCA [CT]^GGCC[GA] EcoT14I 92 CCAAGG C^C[AT][AT]GG HgaI 170 GCGTC GACGC(5/10) HinfI 255 GAATC G^A[ACGT]TC KspI 42 CCGCGG CCGC^GG MboI 260 GATC ^GATC MboII 173 TCTTC GAAGA(8/7) MnlI 136 GAGG CCTC(7/6) MseI 107 TTAA T^TAA MslI 214 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 42 CCGCGG C[AC]G^C[GT]G MvnI 43 CGCG CG^CG NdeII 260 GATC ^GATC NlaIII 342 CATG CATG^ NspV 122 TTCGAA TT^CGAA PmaCI 49 CACGTG CAC^GTG PmlI 49 CACGTG CAC^GTG SacII 42 CCGCGG CCGC^GG Sau3AI 260 GATC ^GATC SfuI 122 TTCGAA TT^CGAA StyI 92 CCAAGG C^C[AT][AT]GG TfiI 255 GAATC G^A[AT]TC Tru9I 107 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AluI 197 AGCT AG^CT 209 AGCT BmyI 77 GTGCCC G[AGT]GC[ACT]^C 88 GTGCCC BsaJI 42 CCGCGG C^C[ACGT][ACGT]GG 92 CCAAGG BsgI 66 CTGCAC GTGCAG(16/14) 52 GTGCAG BsmFI 152 GGGAC GGGAC(10/14) 326 GTCCC Bsp1286I 77 GTGCCC G[AGT]GC[ACT]^C 88 GTGCCC Cfr13I 39 GGGCC G^G[ACGT]CC 279 GGACC FauI 272 CCCGC CCCGC(4/6) 310 GCGGG MaeII 50 ACGT A^CGT 324 ACGT MwoI 189 GCTTCCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 310 GCGGGTGCTGC Sau96I 39 GGGCC G^G[ACGT]CC 279 GGACC SfaNI 83 GATGC GCATC(5/9) 178 GATGC Tsp509I 113 AATT ^AATT 234 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI BbvI Cac8I Fnu4HI HaeIII ItaI TaqI TseI TthHB8I