Restriction map for
pFN21AE2236
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI HaeII HapII
HgaI Hin1I HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI MwoI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 970 GAATTT [GA]^AATT[CT]
AocI 1300 CCTAAGG CC^T[ACGT]AGG
ApoI 970 GAATTT [GA]^AATT[CT]
AspHI 730 GAGCTC G[AT]GC[AT]^C
AviII 257 TGCGCA TGC^GCA
BalI 657 TGGCCA TGG^CCA
BseRI 76 GAGGAG GAGGAG(10/8)
BsiHKAI 730 GAGCTC G[AT]GC[AT]^C
BsmFI 1043 GGGAC GGGAC(10/14)
BspHI 648 TCATGA T^CATGA
BspMI 1105 GCAGGT ACCTGC(4/8)
BsrDI 1423 CATTGC GCAATG(2/0)
Bsu36I 1300 CCTAAGG CC^T[ACGT]AGG
CfoI 258 GCGC GCG^C
DsaI 1247 CCATGG C^C[GA][CT]GG
EaeI 657 TGGCCA [CT]^GGCC[GA]
Eco81I 1300 CCTAAGG CC^T[ACGT]AGG
EcoNI 129 CCTCGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 257 TGCGCA TGC^GCA
HhaI 258 GCGC GCG^C
HinP1I 258 GCGC G^CGC
HincII 1033 GTTAAC GT[CT]^[GA]AC
HindII 1033 GTTAAC GT[CT]^[GA]AC
HindIII 695 AAGCTT A^AGCTT
HpaI 1033 GTTAAC GTT^AAC
MaeII 284 ACGT A^CGT
MluNI 657 TGGCCA TGG^CCA
MscI 657 TGGCCA TGG^CCA
MslI 583 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1247 CCATGG C^CATGG
NdeI 1025 CATATG CA^TATG
NspI 311 GCATGC [GA]CATG^[CT]
PshAI 283 GACGTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 648 TCATGA T^CATGA
SacI 730 GAGCTC GAGCT^C
ScaI 119 AGTACT AGT^ACT
SphI 311 GCATGC GCATG^C
TfiI 388 GATTC G^A[AT]TC
XcmI 1376 CCAATGCCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 744 CCGC CCGC(-3/-1)
1210 GCGG
AvaII 559 GGACC G^G[AT]CC
787 GGACC
BanI 622 GGCACC G^G[CT][GA]CC
819 GGTGCC
BanII 670 GGGCTC G[GA]GC[CT]^C
730 GAGCTC
BsmAI 503 GAGAC GTCTC(1/5)
777 GTCTC
DraI 922 TTTAAA TTT^AAA
1060 TTTAAA
EarI 1001 CTCTTC CTCTTC(1/4)
421 GAAGAG
EcoT22I 4 ATGCAT ATGCA^T
614 ATGCAT
Ksp632I 1001 CTCTTC CTCTTC(1/4)
421 GAAGAG
NsiI 4 ATGCAT ATGCA^T
614 ATGCAT
PleI 82 GACTC GAGTC(4/5)
429 GACTC
PvuII 1166 CAGCTG CAG^CTG
1413 CAGCTG
TaqI 131 TCGA T^CGA
884 TCGA
TthHB8I 131 TCGA T^CGA
884 TCGA
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI NdeII NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII