(Circular) (Six-base) MAPSORT of: pFN21AE2236.seq Check: 4842 from: 1 to: 6133 pFN21AE2236 6133 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 2718 3334 4196 5580 5848 1632 Size: 652 434 616 862 1384 268 1917 Fragments arranged by size: 1917 1384 862 652 616 434 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 5032 6009 1203 Size: 3829 977 1327 Fragments arranged by size: 3829 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 3876 5448 3876 Size: 1572 4561 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 2975 3442 3668 3820 5929 1493 Size: 1482 467 226 152 2109 1697 Fragments arranged by size: 2109 1697 1482 467 226 152 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 3458 1870 Size: 73 1515 4545 Fragments arranged by size: 4545 1515 73 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2627 2824 3454 4909 618 Size: 325 217 627 840 197 630 1455 1842 Fragments arranged by size: 1842 1455 840 630 627 325 217 197 BanII G_rGCy'C Cuts at: 1391 2679 2739 3452 1391 Size: 1288 60 713 4072 Fragments arranged by size: 4072 1288 713 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BfrBI ATG'CAT Cuts at: 2011 2621 2011 Size: 610 5523 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6128 6128 Size: 6133 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2829 4194 5350 1790 Size: 1039 1365 1156 2573 Fragments arranged by size: 2573 1365 1156 1039 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2737 3864 4822 Size: 416 53 83 399 24 1320 1127 958 Cuts at: 4822 442 Size: 1753 Fragments arranged by size: 1753 1320 1127 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2273 2434 3247 4793 1239 Size: 1034 161 813 1546 2579 Fragments arranged by size: 2579 1546 1034 813 161 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 5123 5421 4258 Size: 865 298 4970 Fragments arranged by size: 4970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4796 882 Size: 617 3297 2219 Fragments arranged by size: 3297 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3924 493 Size: 711 2720 2702 Fragments arranged by size: 2720 2702 711 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3940 4322 4957 275 Size: 53 83 186 936 2407 382 635 1451 Fragments arranged by size: 2407 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4566 4964 5238 5385 5796 4566 Size: 398 274 147 411 4903 Fragments arranged by size: 4903 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2288 2318 1178 Size: 30 165 30 885 30 4993 Fragments arranged by size: 4993 885 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 1186 Size: 910 5223 BseYI C'CCAG_C Cuts at: 2106 5336 5832 2106 Size: 3230 496 2407 Fragments arranged by size: 3230 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 4194 4279 5350 2739 Size: 1455 85 1071 3522 Fragments arranged by size: 3522 1455 1071 85 BsmI GAATG_Cn' Cuts at: 2018 2205 2775 3644 3737 2018 Size: 187 570 869 93 4414 Fragments arranged by size: 4414 869 570 187 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2679 2739 2829 3452 4194 4279 5350 Size: 399 889 60 90 623 742 85 1071 Cuts at: 5350 1391 Size: 2174 Fragments arranged by size: 2174 1071 889 742 623 399 90 85 60 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 2653 4022 5752 2653 Size: 1369 1730 3034 Fragments arranged by size: 3034 1730 1369 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 3488 844 Size: 2257 387 3489 Fragments arranged by size: 3489 2257 387 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3427 4628 4802 65 Size: 1183 2179 1201 174 1396 Fragments arranged by size: 2179 1396 1201 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4777 4964 1700 Size: 110 2967 187 2869 Fragments arranged by size: 2967 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstYI r'GATC_y Cuts at: 1972 2098 2849 2951 3463 3830 4215 4232 5684 Size: 126 751 102 512 367 385 17 1452 Cuts at: 5684 5770 5782 6128 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 751 512 385 367 346 126 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3306 3306 Size: 6133 BtgI C'CryG_G Cuts at: 513 1065 3252 513 Size: 552 2187 3394 Fragments arranged by size: 3394 2187 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 3649 4443 4463 3649 Size: 794 20 5319 Fragments arranged by size: 5319 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 2929 3067 3437 2929 Size: 138 370 5625 Fragments arranged by size: 5625 370 138 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2662 3580 4469 Size: 54 1425 50 275 18 832 918 889 Cuts at: 4469 6098 8 Size: 1629 43 Fragments arranged by size: 1629 1425 918 889 832 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2421 3012 4063 1353 Size: 1068 591 1051 3423 Fragments arranged by size: 3423 1068 1051 591 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2738 4720 5094 5240 387 Size: 1064 138 1149 1982 374 146 1280 Fragments arranged by size: 1982 1280 1149 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2737 2737 Size: 6133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2284 2434 2718 3247 3334 4196 Size: 393 641 11 150 284 529 87 862 Cuts at: 4196 4793 5580 5848 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 862 787 641 597 529 393 284 268 150 87 11 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2138 Size: 6133 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 2264 4639 2264 Size: 2375 3758 HaeII r_GCGC'y Cuts at: 1678 5280 1678 Size: 3602 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 4774 4806 4848 Size: 32 135 32 929 32 2241 32 42 Cuts at: 4848 4880 1373 Size: 32 2626 Fragments arranged by size: 2626 2241 929 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3040 3477 3723 669 Size: 988 281 1102 437 246 3079 Fragments arranged by size: 3079 1102 988 437 281 246 HindIII A'AGCT_T Cuts at: 748 2700 748 Size: 1952 4181 HpaI GTT'AAC Cuts at: 3040 3723 3040 Size: 683 5450 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 2199 3173 5247 5431 1305 Size: 810 84 974 2074 184 2007 Fragments arranged by size: 2074 2007 974 810 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 2664 10 Size: 54 1425 343 832 3479 Fragments arranged by size: 3479 1425 832 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2592 4091 4450 4609 518 Size: 648 1426 1499 359 159 2042 Fragments arranged by size: 2042 1499 1426 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3173 3215 3420 3549 4226 5374 5619 1606 Size: 1567 42 205 129 677 1148 245 2120 Fragments arranged by size: 2120 1567 1148 677 245 205 129 42 NcoI C'CATG_G Cuts at: 513 1065 3252 513 Size: 552 2187 3394 Fragments arranged by size: 3394 2187 552 NdeI CA'TA_TG Cuts at: 387 3031 387 Size: 2644 3489 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NsiI A_TGCA'T Cuts at: 2013 2623 2013 Size: 610 5523 NspI r_CATG'y Cuts at: 1132 1229 2320 3491 5036 1132 Size: 97 1091 1171 1545 2229 Fragments arranged by size: 2229 1545 1171 1091 97 PasI CC'CwG_GG Cuts at: 1348 2671 3136 1348 Size: 1323 465 4345 Fragments arranged by size: 4345 1323 465 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 3136 3253 1108 Size: 1563 465 117 3988 Fragments arranged by size: 3988 1563 465 117 PfoI T'CCnGG_A Cuts at: 3292 3292 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2292 1613 Size: 679 5454 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 3485 830 Size: 2655 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 3173 3420 3173 Size: 247 5886 SacI G_AGCT'C Cuts at: 2739 2739 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 2126 4381 6093 2126 Size: 2255 1712 2166 Fragments arranged by size: 2255 2166 1712 SfcI C'TryA_G Cuts at: 826 1045 3481 4616 5297 5488 826 Size: 219 2436 1135 681 191 1471 Fragments arranged by size: 2436 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4237 5138 5400 820 Size: 197 926 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 2320 3491 1132 Size: 1188 1171 3774 Fragments arranged by size: 3774 1188 1171 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2194 2212 3252 3572 513 Size: 552 1129 18 1040 320 3074 Fragments arranged by size: 3074 1129 1040 552 320 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4160 4177 4330 4515 4960 325 Size: 1255 2580 17 153 185 445 1498 Fragments arranged by size: 2580 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 4379 6091 Size: 274 80 33 51 1443 147 2255 1712 Cuts at: 6091 96 Size: 138 Fragments arranged by size: 2255 1712 1443 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2288 2320 6093 6125 2288 Size: 32 3773 32 2296 Fragments arranged by size: 3773 2296 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3388 3388 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI