Restriction map for
pFN21AB5013
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# NO CUT ENZYMES ( 162 enzymes) #
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Acc65I AccI AccIII AclI AfaI
AflII AflIII AhdI Alw44I AlwI
AlwNI AocI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 60 GACGTC GACGT^C
AccII 10 CGCG CG^CG
AcsI 525 AAATTC [GA]^AATT[CT]
AgeI 419 ACCGGT A^CCGGT
Aor51HI 203 AGCGCT AGC^GCT
ApoI 525 AAATTC [GA]^AATT[CT]
AseI 484 ATTAAT AT^TAAT
AsnI 484 ATTAAT AT^TAAT
Asp700I 435 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 368 TGGCCA TGG^CCA
BanI 351 GGTGCC G^G[CT][GA]CC
BcnI 405 CCGGG CC^[GC]GG
BfaI 316 CTAG C^TAG
BmyI 352 GTGCCC G[AGT]GC[ACT]^C
BsaWI 419 ACCGGT [AT]^CCGG[AT]
BsgI 70 GTGCAG GTGCAG(16/14)
Bsp1286I 352 GTGCCC G[AGT]GC[ACT]^C
BsrI 147 CCAGT ACTGG(1/-1)
BstF5I 237 GGATG GGATG(2/0)
BstNI 84 CCAGG CC^[AT]GG
BstUI 10 CGCG CG^CG
CfoI 204 GCGC GCG^C
DpnII 382 GATC ^GATC
DraII 311 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 113 CCACGG C^C[GA][CT]GG
EaeI 368 TGGCCA [CT]^GGCC[GA]
Eco47III 203 AGCGCT AGC^GCT
EcoO109I 311 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 84 CCAGG ^CC[AT]GG
EcoRV 538 GATATC GAT^ATC
FokI 237 GGATG GGATG(9/13)
HaeII 203 AGCGCT [GA]GCGC^[CT]
HaeIII 369 GGCC GG^CC
HgaI 43 GACGC GACGC(5/10)
HhaI 204 GCGC GCG^C
HinP1I 204 GCGC G^CGC
HinfI 500 GAGTC G^A[ACGT]TC
HphI 209 TCACC GGTGA(8/7)
MaeI 316 CTAG C^TAG
MaeIII 268 GTTAC ^GT[ACGT]AC
MboI 382 GATC ^GATC
MboII 535 GAAGA GAAGA(8/7)
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MseI 485 TTAA T^TAA
MslI 210 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 84 CCAGG CC^[AT]GG
MvnI 10 CGCG CG^CG
NciI 405 CCGGG CC^[GC]GG
NdeII 382 GATC ^GATC
NlaIII 543 CATG CATG^
PinAI 419 ACCGGT A^CCGGT
PleI 500 GAGTC GAGTC(4/5)
PpuMI 311 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 484 ATTAAT AT^TAAT
Sau3AI 382 GATC ^GATC
SfaNI 238 GATGC GCATC(5/9)
SspI 481 AATATT AAT^ATT
Tru9I 485 TTAA T^TAA
Tsp509I 526 AATT ^AATT
XcmI 113 CCACGGTGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 435 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 43 GACGCC G[GA]^CG[CT]C
60 GACGTC
BpmI 145 CTCCAG CTGGAG(16/14)
591 CTCCAG
BsaHI 43 GACGCC G[GA]^CG[CT]C
60 GACGTC
BsrFI 348 GCCGGT [GA]^CCGG[CT]
419 ACCGGT
BssKI 84 CCAGG ^CC[ACGT]GG
405 CCGGG
Cac8I 323 GCAAGC GC[ACGT]^[ACGT]GC
359 GCCTGC
Cfr10I 348 GCCGGT [GA]^CCGG[CT]
419 ACCGGT
EcoT14I 391 CCTTGG C^C[AT][AT]GG
558 CCAAGG
Hin1I 43 GACGCC G[GA]^CG[CT]C
60 GACGTC
MaeII 61 ACGT A^CGT
266 ACGT
MspA1I 178 CAGCTG C[AC]G^C[GT]G
594 CAGCTG
NlaIV 351 GGTGCC GG[ACGT]^[ACGT]CC
407 GGGACC
PvuII 178 CAGCTG CAG^CTG
594 CAGCTG
ScrFI 84 CCAGG CC^[ACGT]GG
405 CCGGG
StyI 391 CCTTGG C^C[AT][AT]GG
558 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BsmFI Cfr13I Fnu4HI
HapII HpaII ItaI MnlI MspI
MwoI Sau96I TseI TspRI