(Circular) (Six-base) MAPSORT of: pFN21AB5013.seq Check: 5417 from: 1 to: 5314 pFN21AB5013 5314 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2069 3124 278 Size: 53 83 186 936 533 1055 2468 Fragments arranged by size: 2468 1055 936 533 186 83 53 AccI GT'mk_AC Cuts at: 1937 2657 1937 Size: 720 4594 Acc65I G'GTAC_C Cuts at: 2635 2635 Size: 5314 AclI AA'CG_TT Cuts at: 1563 3441 3814 1563 Size: 1878 373 3063 Fragments arranged by size: 3063 1878 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2083 2162 2165 2275 3377 4761 5029 1632 Size: 451 79 3 110 1102 1384 268 1917 Fragments arranged by size: 1917 1384 1102 451 268 110 79 3 AfeI AGC'GCT Cuts at: 2210 2210 Size: 5314 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5117 AflIII A'CryG_T Cuts at: 1203 4213 5190 1203 Size: 3010 977 1327 Fragments arranged by size: 3010 1327 977 AgeI A'CCGG_T Cuts at: 2424 4145 2424 Size: 1721 3593 AhdI GACnn_n'nnGTC Cuts at: 4043 4043 Size: 5314 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5314 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5314 AlwNI CAG_nnn'CTG Cuts at: 3057 4629 3057 Size: 1572 3742 ApaLI G'TGCA_C Cuts at: 3371 4527 3371 Size: 1156 4158 ApoI r'AATT_y Cuts at: 1493 2530 2623 2849 3001 5110 1493 Size: 1037 93 226 152 2109 1697 Fragments arranged by size: 2109 1697 1037 226 152 93 AseI AT'TA_AT Cuts at: 160 1032 2490 3868 160 Size: 872 1458 1378 1606 Fragments arranged by size: 1606 1458 1378 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 AvaI C'yCGr_G Cuts at: 1870 1943 2639 1870 Size: 73 696 4545 Fragments arranged by size: 4545 696 73 BamHI G'GATC_C Cuts at: 2644 3011 2644 Size: 367 4947 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2356 2635 4090 618 Size: 325 217 627 569 279 1455 1842 Fragments arranged by size: 1842 1455 627 569 325 279 217 BanII G_rGCy'C Cuts at: 1391 2633 1391 Size: 1242 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4343 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3505 3539 1903 Size: 34 1568 34 3678 Fragments arranged by size: 3678 1568 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3206 4416 3206 Size: 1210 4104 BclI T'GATC_A Cuts at: 1548 1548 Size: 5314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3925 136 Size: 107 122 71 631 605 2253 1525 Fragments arranged by size: 2253 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5309 5309 Size: 5314 BlpI GC'TnA_GC Cuts at: 2731 2731 Size: 5314 Bme1580I G_kGCm'C Cuts at: 1790 2361 3375 4531 1790 Size: 571 1014 1156 2573 Fragments arranged by size: 2573 1156 1014 571 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3045 4003 442 Size: 416 53 83 399 24 1628 958 1753 Fragments arranged by size: 1753 1628 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5314 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2581 3974 1239 Size: 896 446 1393 2579 Fragments arranged by size: 2579 1393 896 446 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3439 4304 4602 3439 Size: 865 298 4151 Fragments arranged by size: 4151 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3977 882 Size: 617 2478 2219 Fragments arranged by size: 2478 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3105 493 Size: 711 1901 2702 Fragments arranged by size: 2702 1901 711 BsaBI GATnn'nnATC Cuts at: 5308 5308 Size: 5314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2066 3121 3503 Size: 53 83 186 936 516 17 1055 382 Cuts at: 3503 4138 275 Size: 635 1451 Fragments arranged by size: 1451 1055 936 635 516 382 186 83 53 17 BsaWI w'CCGG_w Cuts at: 2424 3747 4145 4419 4566 4977 2424 Size: 1323 398 274 147 411 2761 Fragments arranged by size: 2761 1323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2340 2370 1178 Size: 30 165 30 937 30 4122 Fragments arranged by size: 4122 937 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5314 BseYI C'CCAG_C Cuts at: 4517 5013 4517 Size: 496 4818 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 1163 Size: 573 360 4381 Fragments arranged by size: 4381 573 360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2764 3525 4553 1540 Size: 275 186 763 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3375 3460 4531 3375 Size: 85 1071 4158 Fragments arranged by size: 4158 1071 85 BsmI GAATG_Cn' Cuts at: 2825 2918 2825 Size: 93 5221 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2633 3375 3460 4531 1391 Size: 399 571 272 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 399 272 85 BspEI T'CCGG_A Cuts at: 4977 4977 Size: 5314 BspHI T'CATG_A Cuts at: 3203 4933 3203 Size: 1730 3584 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 844 Size: 1825 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3201 1394 Size: 429 1378 3507 Fragments arranged by size: 3507 1378 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3809 3983 65 Size: 1183 2561 174 1396 Fragments arranged by size: 2561 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2353 2424 3958 4145 1700 Size: 110 543 71 1534 187 2869 Fragments arranged by size: 2869 1534 543 187 110 71 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5314 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5314 BssSI C'ACGA_G Cuts at: 3374 4386 3374 Size: 1012 4302 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5314 BstBI TT'CG_AA Cuts at: 4118 4118 Size: 5314 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5314 BstYI r'GATC_y Cuts at: 1972 2644 3011 3396 3413 4865 4951 4963 5309 Size: 672 367 385 17 1452 86 12 346 Cuts at: 5309 1972 Size: 1977 Fragments arranged by size: 1977 1452 672 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 513 Size: 552 939 114 3709 Fragments arranged by size: 3709 939 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4575 Fragments arranged by size: 4575 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2409 2830 3624 3644 2061 Size: 348 421 794 20 3731 Fragments arranged by size: 3731 794 421 348 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5279 DraI TTT'AAA Cuts at: 2618 2618 Size: 5314 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4321 809 Size: 507 3005 1802 Fragments arranged by size: 3005 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2761 3650 Size: 54 1425 50 275 18 543 388 889 Cuts at: 3650 5279 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 543 388 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2761 1537 Size: 275 949 4090 Fragments arranged by size: 4090 949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3244 1353 Size: 1891 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3901 4275 4421 387 Size: 1064 138 2312 374 146 1280 Fragments arranged by size: 2312 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2135 2162 2165 2275 2581 3377 Size: 393 451 52 27 3 110 306 796 Cuts at: 3377 3974 4761 5029 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 796 787 597 451 393 306 268 110 52 27 3 EcoO109I rG'GnC_Cy Cuts at: 2317 2317 Size: 5314 EcoRI G'AATT_C Cuts at: 2623 3001 2623 Size: 378 4936 EcoRV GAT'ATC Cuts at: 2545 2545 Size: 5314 FspI TGC'GCA Cuts at: 3820 3820 Size: 5314 HaeII r_GCGC'y Cuts at: 1678 2212 4461 1678 Size: 534 2249 2531 Fragments arranged by size: 2531 2249 534 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 3955 3987 4029 Size: 32 135 32 962 32 1389 32 42 Cuts at: 4029 4061 1373 Size: 32 2626 Fragments arranged by size: 2626 1389 962 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2658 2904 669 Size: 988 281 720 246 3079 Fragments arranged by size: 3079 988 720 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5314 HpaI GTT'AAC Cuts at: 2904 2904 Size: 5314 KpnI G_GTAC'C Cuts at: 2639 2639 Size: 5314 MfeI C'AATT_G Cuts at: 2913 2913 Size: 5314 MluI A'CGCG_T Cuts at: 5190 5190 Size: 5314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2435 4428 4612 1305 Size: 1130 1993 184 2007 Fragments arranged by size: 2007 1993 1130 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 2949 Fragments arranged by size: 2949 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 3272 3631 3790 518 Size: 648 1053 1053 359 159 2042 Fragments arranged by size: 2042 1053 1053 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 2601 2730 3407 4555 4800 1606 Size: 579 416 129 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 579 416 245 129 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3823 Fragments arranged by size: 3823 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5314 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5314 NotI GC'GGCC_GC Cuts at: 2761 2761 Size: 5314 NspI r_CATG'y Cuts at: 1132 1229 2672 4217 1132 Size: 97 1443 1545 2229 Fragments arranged by size: 2229 1545 1443 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5314 PciI A'CATG_T Cuts at: 4213 4213 Size: 5314 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5314 PmeI GTTT'AAAC Cuts at: 2618 2618 Size: 5314 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3383 3415 4896 4928 3383 Size: 32 1481 32 3769 Fragments arranged by size: 3769 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2317 2317 Size: 5314 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5314 PsiI TTA'TAA Cuts at: 2884 2884 Size: 5314 PstI C_TGCA'G Cuts at: 830 2666 830 Size: 1836 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5314 PvuII CAG'CTG Cuts at: 2185 2601 2185 Size: 416 4898 SalI G'TCGA_C Cuts at: 1936 2656 1936 Size: 720 4594 SbfI CC_TGCA'GG Cuts at: 2666 2666 Size: 5314 ScaI AGT'ACT Cuts at: 3562 5274 3562 Size: 1712 3602 SfcI C'TryA_G Cuts at: 826 1045 2662 3797 4478 4669 826 Size: 219 1617 1135 681 191 1471 Fragments arranged by size: 1617 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5314 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5314 SmaI CCC'GGG Cuts at: 1872 2641 1872 Size: 769 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3418 4319 4581 820 Size: 197 926 1475 901 262 1553 Fragments arranged by size: 1553 1475 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3105 493 Size: 2612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5314 SphI G_CATG'C Cuts at: 1132 2672 1132 Size: 1540 3774 SspI AAT'ATT Cuts at: 5 52 2488 3238 5 Size: 47 2436 750 2081 Fragments arranged by size: 2436 2081 750 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5314 StyI C'CwwG_G Cuts at: 513 1065 2004 2396 2563 2753 513 Size: 552 939 392 167 190 3074 Fragments arranged by size: 3074 939 552 392 190 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2576 3341 3358 3511 3696 4141 325 Size: 1255 996 765 17 153 185 445 1498 Fragments arranged by size: 1498 1255 996 765 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3560 5272 96 Size: 274 80 33 51 1443 1583 1712 138 Fragments arranged by size: 1712 1583 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2099 2131 2340 2372 5274 5306 2099 Size: 32 209 32 2902 32 2107 Fragments arranged by size: 2902 2107 209 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5314 XbaI T'CTAG_A Cuts at: 2650 2650 Size: 5314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2125 Size: 5314 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5314 XmaI C'CCGG_G Cuts at: 1870 2639 1870 Size: 769 4545 XmnI GAAnn'nnTTC Cuts at: 2444 3443 2444 Size: 999 4315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2067 3122 276 Size: 53 83 186 936 533 1055 2468 Fragments arranged by size: 2468 1055 936 533 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI