################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BbsI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MspA1I MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 698 CCGC CCGC(-3/-1) Aor51HI 459 AGCGCT AGC^GCT BamHI 48 GGATCC G^GATCC BanII 732 GAGCCC G[GA]GC[CT]^C BbvCI 401 CCTCAGC CCTCAGC(-5/-2) BciVI 336 GGATAC GTATCC(6/5) BfaI 316 CTAG C^TAG BmyI 732 GAGCCC G[AGT]GC[ACT]^C BpmI 775 CTCCAG CTGGAG(16/14) BsaI 254 GGTCTC GGTCTC(1/5) BsgI 720 CTGCAC GTGCAG(16/14) BsmI 273 GAATGC GAATGC(1/-1) Bsp1286I 732 GAGCCC G[AGT]GC[ACT]^C BspDI 646 ATCGAT AT^CGAT BssHII 689 GCGCGC G^CGCGC BstYI 48 GGATCC [GA]^GATC[CT] ClaI 646 ATCGAT AT^CGAT DraII 704 AGGGCCT [GA]G^G[ACGT]CC[CT] Eco47III 459 AGCGCT AGC^GCT EcoO109I 704 AGGGCCT [GA]G^G[ACGT]CC[CT] HaeII 459 AGCGCT [GA]GCGC^[CT] HphI 637 GGTGA GGTGA(8/7) MaeI 316 CTAG C^TAG MaeIII 638 GTGAC ^GT[ACGT]AC MflI 48 GGATCC [GA]^GATC[CT] MseI 656 TTAA T^TAA MslI 492 CATAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PstI 725 CTGCAG CTGCA^G SfcI 725 CTGCAG C^T[GA][CT]AG TaqI 647 TCGA T^CGA TfiI 524 GAATC G^A[AT]TC Tru9I 656 TTAA T^TAA Tsp45I 638 GTGAC ^GT[GC]AC TthHB8I 647 TCGA T^CGA XhoII 48 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 688 CGCG CG^CG 690 CGCG BalI 42 TGGCCA TGG^CCA 752 TGGCCA BsmFI 171 GGGAC GGGAC(10/14) 590 GTCCC BsrI 757 ACTGG ACTGG(1/-1) 131 CCAGT BstF5I 154 CATCC GGATG(2/0) 21 GGATG BstUI 688 CGCG CG^CG 690 CGCG BstXI 191 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 777 CCAGCCCCATGG Cfr13I 673 GGCCC G^G[ACGT]CC 705 GGGCC DdeI 140 CTGAG C^T[ACGT]AG 402 CTCAG DsaI 482 CCATGG C^C[GA][CT]GG 783 CCATGG EarI 125 CTCTTC CTCTTC(1/4) 413 CTCTTC FokI 154 CATCC GGATG(9/13) 21 GGATG HindIII 145 AAGCTT A^AGCTT 370 AAGCTT Ksp632I 125 CTCTTC CTCTTC(1/4) 413 CTCTTC MluNI 42 TGGCCA TGG^CCA 752 TGGCCA MscI 42 TGGCCA TGG^CCA 752 TGGCCA MvnI 688 CGCG CG^CG 690 CGCG NcoI 482 CCATGG C^CATGG 783 CCATGG NlaIII 483 CATG CATG^ 784 CATG PleI 173 GACTC GAGTC(4/5) 397 GAGTC Sau96I 673 GGCCC G^G[ACGT]CC 705 GGGCC SmlI 463 CTCAAG C^T[CT][GA]AG 613 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsiYI BslI BsmAI BssKI BstNI Cac8I CfoI DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIV Sau3AI ScrFI SfaNI StyI TseI Tsp509I TspRI