(Circular) (Six-base) MAPSORT of: pFN21AE6215.seq Check: 6777 from: 1 to: 5500 pFN21AE6215 5500 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3310 278 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2843 1937 Size: 906 4594 Acc65I G'GTAC_C Cuts at: 2821 2821 Size: 5500 AclI AA'CG_TT Cuts at: 1563 3627 4000 1563 Size: 2064 373 3063 Fragments arranged by size: 3063 2064 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2184 2265 3563 4947 5215 1632 Size: 410 142 81 1298 1384 268 1917 Fragments arranged by size: 1917 1384 1298 410 268 142 81 AfeI AGC'GCT Cuts at: 2466 2466 Size: 5500 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5303 AflIII A'CryG_T Cuts at: 1203 4399 5376 1203 Size: 3196 977 1327 Fragments arranged by size: 3196 1327 977 AgeI A'CCGG_T Cuts at: 4331 4331 Size: 5500 AhdI GACnn_n'nnGTC Cuts at: 4229 4229 Size: 5500 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5500 AlwNI CAG_nnn'CTG Cuts at: 3243 4815 3243 Size: 1572 3928 ApaLI G'TGCA_C Cuts at: 3557 4713 3557 Size: 1156 4344 ApoI r'AATT_y Cuts at: 1493 2809 3035 3187 5296 1493 Size: 1316 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 226 152 AseI AT'TA_AT Cuts at: 160 1032 4054 160 Size: 872 3022 1606 Fragments arranged by size: 3022 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 AvaI C'yCGr_G Cuts at: 1870 1943 2825 1870 Size: 73 882 4545 Fragments arranged by size: 4545 882 73 BamHI G'GATC_C Cuts at: 2053 2830 3197 2053 Size: 777 367 4356 Fragments arranged by size: 4356 777 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2821 4276 618 Size: 325 217 627 1034 1455 1842 Fragments arranged by size: 1842 1455 1034 627 325 217 BanII G_rGCy'C Cuts at: 1391 2741 2819 1391 Size: 1350 78 4072 Fragments arranged by size: 4072 1350 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4529 BbvCI CC'TCA_GC Cuts at: 2407 2407 Size: 5500 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3691 3725 1903 Size: 34 1754 34 3678 Fragments arranged by size: 3678 1754 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2335 3392 4602 2335 Size: 1057 1210 3233 Fragments arranged by size: 3233 1210 1057 BclI T'GATC_A Cuts at: 1548 1548 Size: 5500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4111 136 Size: 107 122 71 631 605 2439 1525 Fragments arranged by size: 2439 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5495 5495 Size: 5500 BlpI GC'TnA_GC Cuts at: 2917 2917 Size: 5500 Bme1580I G_kGCm'C Cuts at: 1790 3561 4717 1790 Size: 1771 1156 2573 Fragments arranged by size: 2573 1771 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5500 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 3231 4189 Size: 416 53 83 399 24 713 1101 958 Cuts at: 4189 442 Size: 1753 Fragments arranged by size: 1753 1101 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 4160 1239 Size: 1526 1395 2579 Fragments arranged by size: 2579 1526 1395 Bpu10I CC'TnA_GC Cuts at: 2407 2407 Size: 5500 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2453 2603 3625 4490 4788 2453 Size: 150 1022 865 298 3165 Fragments arranged by size: 3165 1022 865 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2265 4163 882 Size: 617 766 1898 2219 Fragments arranged by size: 2219 1898 766 617 BsaAI yAC'GTr Cuts at: 493 1204 3291 493 Size: 711 2087 2702 Fragments arranged by size: 2702 2087 711 BsaBI GATnn'nnATC Cuts at: 5494 5494 Size: 5500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3307 3689 4324 275 Size: 53 83 186 936 1774 382 635 1451 Fragments arranged by size: 1774 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3933 4331 4605 4752 5163 3933 Size: 398 274 147 411 4270 Fragments arranged by size: 4270 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5275 Fragments arranged by size: 5275 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5500 BseYI C'CCAG_C Cuts at: 2559 4703 5199 2559 Size: 2144 496 2860 Fragments arranged by size: 2860 2144 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2710 1163 Size: 573 974 3953 Fragments arranged by size: 3953 974 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2950 3711 4739 1540 Size: 275 186 949 761 1028 2301 Fragments arranged by size: 2301 1028 949 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3561 3646 4717 3561 Size: 85 1071 4344 Fragments arranged by size: 4344 1071 85 BsmI GAATG_Cn' Cuts at: 2284 3011 3104 2284 Size: 727 93 4680 Fragments arranged by size: 4680 727 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2741 2819 3561 3646 4717 1391 Size: 399 951 78 742 85 1071 2174 Fragments arranged by size: 2174 1071 951 742 399 85 78 BspEI T'CCGG_A Cuts at: 5163 5163 Size: 5500 BspHI T'CATG_A Cuts at: 3389 5119 3389 Size: 1730 3770 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2855 844 Size: 2011 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3387 1394 Size: 429 1564 3507 Fragments arranged by size: 3507 1564 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3995 4169 65 Size: 1183 2747 174 1396 Fragments arranged by size: 2747 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4144 4331 1700 Size: 110 2334 187 2869 Fragments arranged by size: 2869 2334 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5500 BssHII G'CGCG_C Cuts at: 1925 2694 1925 Size: 769 4731 BssSI C'ACGA_G Cuts at: 3560 4572 3560 Size: 1012 4488 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5500 BstBI TT'CG_AA Cuts at: 4304 4304 Size: 5500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5500 BstXI CCAn_nnnn'nTGG Cuts at: 2203 2789 2203 Size: 586 4914 BstYI r'GATC_y Cuts at: 1972 2053 2830 3197 3582 3599 5051 5137 5149 Size: 81 777 367 385 17 1452 86 12 Cuts at: 5149 5495 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 777 385 367 346 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2487 2788 513 Size: 552 1422 301 3225 Fragments arranged by size: 3225 1422 552 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4761 Fragments arranged by size: 4761 683 56 BtsI GCAGTG_nn' Cuts at: 2208 2411 2727 3016 3810 3830 2208 Size: 203 316 289 794 20 3878 Fragments arranged by size: 3878 794 316 289 203 20 ClaI AT'CG_AT Cuts at: 1554 2652 1554 Size: 1098 4402 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5465 DraI TTT'AAA Cuts at: 2804 2804 Size: 5500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4507 809 Size: 507 3191 1802 Fragments arranged by size: 3191 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2700 2757 Size: 54 1425 50 275 18 217 653 57 Cuts at: 2757 2947 3836 5465 8 Size: 190 889 1629 43 Fragments arranged by size: 1629 1425 889 653 275 217 190 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2947 1537 Size: 275 1135 4090 Fragments arranged by size: 4090 1135 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2424 3430 1353 Size: 783 288 1006 3423 Fragments arranged by size: 3423 1006 783 288 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4087 4461 4607 387 Size: 1064 138 2498 374 146 1280 Fragments arranged by size: 2498 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2184 2265 2765 3563 4160 4947 Size: 393 410 142 81 500 798 597 787 Cuts at: 4947 5215 1239 Size: 268 1524 Fragments arranged by size: 1524 798 787 597 500 410 393 268 142 81 EcoO109I rG'GnC_Cy Cuts at: 2710 2710 Size: 5500 EcoRI G'AATT_C Cuts at: 2809 3187 2809 Size: 378 5122 FspI TGC'GCA Cuts at: 4006 4006 Size: 5500 HaeII r_GCGC'y Cuts at: 1678 2468 4647 1678 Size: 790 2179 2531 Fragments arranged by size: 2531 2179 790 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 4141 4173 4215 Size: 32 135 32 539 32 1998 32 42 Cuts at: 4215 4247 1373 Size: 32 2626 Fragments arranged by size: 2626 1998 539 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2844 3090 669 Size: 988 281 906 246 3079 Fragments arranged by size: 3079 988 906 281 246 HindIII A'AGCT_T Cuts at: 748 2150 2375 748 Size: 1402 225 3873 Fragments arranged by size: 3873 1402 225 HpaI GTT'AAC Cuts at: 3090 3090 Size: 5500 KpnI G_GTAC'C Cuts at: 2825 2825 Size: 5500 MfeI C'AATT_G Cuts at: 3099 3099 Size: 5500 MluI A'CGCG_T Cuts at: 5376 5376 Size: 5500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2281 4614 4798 1305 Size: 976 2333 184 2007 Fragments arranged by size: 2333 2007 976 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2049 2759 10 Size: 54 1425 343 217 710 2751 Fragments arranged by size: 2751 1425 710 343 217 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 3458 3817 3976 518 Size: 648 1335 957 359 159 2042 Fragments arranged by size: 2042 1335 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2916 3593 4741 4986 1606 Size: 1310 677 1148 245 2120 Fragments arranged by size: 2120 1310 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2487 2788 513 Size: 552 1422 301 3225 Fragments arranged by size: 3225 1422 552 301 NdeI CA'TA_TG Cuts at: 387 387 Size: 5500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5500 NotI GC'GGCC_GC Cuts at: 2947 2947 Size: 5500 NspI r_CATG'y Cuts at: 1132 1229 2858 4403 1132 Size: 97 1629 1545 2229 Fragments arranged by size: 2229 1629 1545 97 PasI CC'CwG_GG Cuts at: 1348 2707 1348 Size: 1359 4141 PciI A'CATG_T Cuts at: 4399 4399 Size: 5500 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5500 PmeI GTTT'AAAC Cuts at: 2804 2804 Size: 5500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 5082 5114 3569 Size: 32 1481 32 3955 Fragments arranged by size: 3955 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5500 PsiI TTA'TAA Cuts at: 3070 3070 Size: 5500 PstI C_TGCA'G Cuts at: 830 2734 2852 830 Size: 1904 118 3478 Fragments arranged by size: 3478 1904 118 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5500 SalI G'TCGA_C Cuts at: 1936 2842 1936 Size: 906 4594 SbfI CC_TGCA'GG Cuts at: 2852 2852 Size: 5500 ScaI AGT'ACT Cuts at: 3748 5460 3748 Size: 1712 3788 SfcI C'TryA_G Cuts at: 826 1045 2730 2848 3983 4664 4855 826 Size: 219 1685 118 1135 681 191 1471 Fragments arranged by size: 1685 1471 1135 681 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5500 SmaI CCC'GGG Cuts at: 1872 2827 1872 Size: 955 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2468 2618 3604 4505 4767 820 Size: 197 926 525 150 986 901 262 1553 Fragments arranged by size: 1553 986 926 901 525 262 197 150 SnaBI TAC'GTA Cuts at: 493 3291 493 Size: 2798 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5500 SphI G_CATG'C Cuts at: 1132 2858 1132 Size: 1726 3774 SspI AAT'ATT Cuts at: 5 52 3424 5 Size: 47 3372 2081 Fragments arranged by size: 3372 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5500 StyI C'CwwG_G Cuts at: 513 1065 2487 2533 2788 2939 513 Size: 552 1422 46 255 151 3074 Fragments arranged by size: 3074 1422 552 255 151 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2538 3527 3544 3697 3882 4327 325 Size: 1255 958 989 17 153 185 445 1498 Fragments arranged by size: 1498 1255 989 958 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3746 5458 96 Size: 274 80 33 51 1443 1769 1712 138 Fragments arranged by size: 1769 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5460 5492 5460 Size: 32 5468 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5500 XbaI T'CTAG_A Cuts at: 2836 2836 Size: 5500 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5500 XmaI C'CCGG_G Cuts at: 1870 2825 1870 Size: 955 4545 XmnI GAAnn'nnTTC Cuts at: 3629 3629 Size: 5500 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3308 276 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI