(Circular) (Six-base) MAPSORT of: pFN21AB6520.seq Check: 9615 from: 1 to: 5863 pFN21AB6520 5863 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2895 3673 278 Size: 53 83 186 936 1359 778 2468 Fragments arranged by size: 2468 1359 936 778 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 1937 Size: 1269 4594 Acc65I G'GTAC_C Cuts at: 2277 2778 3184 2277 Size: 501 406 4956 Fragments arranged by size: 4956 501 406 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 3926 5310 5578 1632 Size: 1046 1248 1384 268 1917 Fragments arranged by size: 1917 1384 1248 1046 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 2515 2570 4592 2515 Size: 55 2022 3786 Fragments arranged by size: 3786 2022 55 AleI CACnn'nnGTG Cuts at: 1166 2757 1166 Size: 1591 4272 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5863 AlwNI CAG_nnn'CTG Cuts at: 3006 3606 5178 3006 Size: 600 1572 3691 Fragments arranged by size: 3691 1572 600 ApaI G_GGCC'C Cuts at: 2841 2841 Size: 5863 ApaLI G'TGCA_C Cuts at: 2405 3920 5076 2405 Size: 1515 1156 3192 Fragments arranged by size: 3192 1515 1156 ApoI r'AATT_y Cuts at: 1493 3172 3398 3550 5659 1493 Size: 1679 226 152 2109 1697 Fragments arranged by size: 2109 1697 1679 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2387 2395 2647 2834 3188 1870 Size: 73 153 291 8 252 187 354 4545 Fragments arranged by size: 4545 354 291 252 187 153 73 8 BamHI G'GATC_C Cuts at: 2066 2948 3193 3560 2066 Size: 882 245 367 4369 Fragments arranged by size: 4369 882 367 245 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2277 2732 2778 3184 4639 Size: 325 217 627 490 455 46 406 1455 Cuts at: 4639 618 Size: 1842 Fragments arranged by size: 1842 1455 627 490 455 406 325 217 46 BanII G_rGCy'C Cuts at: 1391 2043 2079 2841 3013 3062 3182 1391 Size: 652 36 762 172 49 120 4072 Fragments arranged by size: 4072 762 652 172 120 49 36 BbeI G_GCGC'C Cuts at: 2736 2736 Size: 5863 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 2601 2889 928 Size: 971 261 441 288 3902 Fragments arranged by size: 3902 971 441 288 261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2337 2371 4054 4088 1903 Size: 34 400 34 1683 34 3678 Fragments arranged by size: 3678 1683 400 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2382 4474 Size: 107 122 71 631 605 337 373 2092 Cuts at: 4474 136 Size: 1525 Fragments arranged by size: 2092 1525 631 605 373 337 122 107 71 BglII A'GATC_T Cuts at: 2110 5858 2110 Size: 3748 2115 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 2409 2841 3924 5080 1790 Size: 619 432 1083 1156 2573 Fragments arranged by size: 2573 1156 1083 619 432 BmgBI CAC'GTC Cuts at: 1318 2053 1318 Size: 735 5128 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2431 3016 3594 Size: 416 53 83 399 24 1014 585 578 Cuts at: 3594 4552 442 Size: 958 1753 Fragments arranged by size: 1753 1014 958 585 578 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2930 4523 1239 Size: 926 765 1593 2579 Fragments arranged by size: 2579 1593 926 765 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2065 2480 2552 3988 4853 5151 2065 Size: 415 72 1436 865 298 2777 Fragments arranged by size: 2777 1436 865 415 298 72 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4526 882 Size: 617 3027 2219 Fragments arranged by size: 3027 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2733 2892 3670 4052 Size: 53 83 186 936 1200 159 778 382 Cuts at: 4052 4687 275 Size: 635 1451 Fragments arranged by size: 1451 1200 936 778 635 382 186 159 83 53 BsaWI w'CCGG_w Cuts at: 2306 4296 4694 4968 5115 5526 2306 Size: 1990 398 274 147 411 2643 Fragments arranged by size: 2643 1990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5638 Fragments arranged by size: 5638 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2575 2717 1186 Size: 1389 142 4332 Fragments arranged by size: 4332 1389 142 BseYI C'CCAG_C Cuts at: 2017 2042 2771 2986 5066 5562 2017 Size: 25 729 215 2080 496 2318 Fragments arranged by size: 2318 2080 729 496 215 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2512 3313 4074 5102 1540 Size: 275 186 511 801 761 1028 2301 Fragments arranged by size: 2301 1028 801 761 511 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2409 3062 3924 4009 5080 2079 Size: 330 653 862 85 1071 2862 Fragments arranged by size: 2862 1071 862 653 330 85 BsmI GAATG_Cn' Cuts at: 2965 3374 3467 2965 Size: 409 93 5361 Fragments arranged by size: 5361 409 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2306 2306 Size: 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2079 2409 2841 3013 3062 3182 Size: 399 253 36 330 432 172 49 120 Cuts at: 3182 3924 4009 5080 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 330 253 172 120 85 49 36 BspEI T'CCGG_A Cuts at: 2306 5526 2306 Size: 3220 2643 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3218 844 Size: 2374 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4358 4532 65 Size: 1183 3110 174 1396 Fragments arranged by size: 3110 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2796 4507 4694 1700 Size: 110 986 1711 187 2869 Fragments arranged by size: 2869 1711 986 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2169 2349 794 Size: 1375 180 4308 Fragments arranged by size: 4308 1375 180 BstBI TT'CG_AA Cuts at: 2541 4667 2541 Size: 2126 3737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 3091 3091 Size: 5863 BstYI r'GATC_y Cuts at: 1972 2066 2110 2217 2948 3193 3560 3945 3962 Size: 94 44 107 731 245 367 385 17 Cuts at: 3962 5414 5500 5512 5858 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 731 385 367 346 245 107 94 86 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2941 513 Size: 552 939 937 3435 Fragments arranged by size: 3435 939 937 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 3379 4173 4193 3379 Size: 794 20 5049 Fragments arranged by size: 5049 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 4870 809 Size: 507 1575 1979 1802 Fragments arranged by size: 1979 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3072 3310 Size: 54 1425 50 275 18 180 1062 238 Cuts at: 3310 4199 5828 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1062 889 275 238 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 3793 1353 Size: 908 1532 3423 Fragments arranged by size: 3423 1532 908 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2961 4450 4824 4970 387 Size: 1064 138 1372 1489 374 146 1280 Fragments arranged by size: 1489 1372 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2077 3060 2077 Size: 983 4880 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2678 2930 3926 4523 5310 5578 Size: 393 533 513 252 996 597 787 268 Cuts at: 5578 1239 Size: 1524 Fragments arranged by size: 1524 996 787 597 533 513 393 268 252 EcoO109I rG'GnC_Cy Cuts at: 2391 2711 2838 2391 Size: 320 127 5416 Fragments arranged by size: 5416 320 127 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3121 3121 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 2736 5010 1678 Size: 1058 2274 2531 Fragments arranged by size: 2531 2274 1058 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 2556 2588 2882 Size: 32 135 32 929 32 23 32 294 Cuts at: 2882 2914 4504 4536 4578 4610 1373 Size: 32 1590 32 42 32 2626 Fragments arranged by size: 2626 1590 929 294 135 42 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KasI G'GCGC_C Cuts at: 2732 2732 Size: 5863 KpnI G_GTAC'C Cuts at: 2281 2782 3188 2281 Size: 501 406 4956 Fragments arranged by size: 4956 501 406 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2453 2499 4977 5161 1305 Size: 1148 46 2478 184 2007 Fragments arranged by size: 2478 2007 1148 184 46 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4041 Fragments arranged by size: 4041 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2757 3821 4180 4339 518 Size: 648 885 706 1064 359 159 2042 Fragments arranged by size: 2042 1064 885 706 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2775 3279 3956 5104 5349 1606 Size: 1169 504 677 1148 245 2120 Fragments arranged by size: 2120 1169 1148 677 504 245 NarI GG'CG_CC Cuts at: 2733 2733 Size: 5863 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4372 Fragments arranged by size: 4372 939 552 NdeI CA'TA_TG Cuts at: 387 2122 387 Size: 1735 4128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2666 3221 4766 1132 Size: 97 1437 555 1545 2229 Fragments arranged by size: 2229 1545 1437 555 97 PasI CC'CwG_GG Cuts at: 1348 2746 1348 Size: 1398 4465 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5863 PfoI T'CCnGG_A Cuts at: 2244 2907 2244 Size: 663 5200 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2391 2711 2391 Size: 320 5543 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PspOMI G'GGCC_C Cuts at: 2837 2837 Size: 5863 PstI C_TGCA'G Cuts at: 830 2469 2808 3215 830 Size: 1639 339 407 3478 Fragments arranged by size: 3478 1639 407 339 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2775 2775 Size: 5863 SacI G_AGCT'C Cuts at: 2079 3062 2079 Size: 983 4880 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SanDI GG'GwC_CC Cuts at: 2711 2711 Size: 5863 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SfcI C'TryA_G Cuts at: 826 1045 2465 2804 3211 4346 5027 5218 826 Size: 219 1420 339 407 1135 681 191 1471 Fragments arranged by size: 1471 1420 1135 681 407 339 219 191 SfoI GGC'GCC Cuts at: 2734 2734 Size: 5863 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 2098 2389 2649 2836 3190 1872 Size: 226 291 260 187 354 4545 Fragments arranged by size: 4545 354 291 260 226 187 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 2495 2531 3967 4868 5130 Size: 197 926 137 415 36 1436 901 262 Cuts at: 5130 820 Size: 1553 Fragments arranged by size: 1553 1436 926 901 415 262 197 137 36 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3125 3787 5 Size: 47 3073 662 2081 Fragments arranged by size: 3073 2081 662 47 StuI AGG'CCT Cuts at: 1517 2501 1517 Size: 984 4879 StyI C'CwwG_G Cuts at: 513 1065 2004 3302 513 Size: 552 939 1298 3074 Fragments arranged by size: 3074 1298 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2413 2526 3890 3907 4060 4245 4690 Size: 1255 833 113 1364 17 153 185 445 Cuts at: 4690 325 Size: 1498 Fragments arranged by size: 1498 1364 1255 833 445 185 153 113 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4109 5821 96 Size: 274 80 33 51 1443 2132 1712 138 Fragments arranged by size: 2132 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2933 2965 5823 5855 2933 Size: 32 2858 32 2941 Fragments arranged by size: 2941 2858 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2743 2020 Size: 723 5140 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 2096 2387 2647 2834 3188 1870 Size: 226 291 260 187 354 4545 Fragments arranged by size: 4545 354 291 260 226 187 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2893 3671 276 Size: 53 83 186 936 1359 778 2468 Fragments arranged by size: 2468 1359 936 778 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI