################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 238 GGCGTC G[GA]^CG[CT]C AfaI 433 GTAC GT^AC AgeI 60 ACCGGT A^CCGGT BanII 117 GAGCCC G[GA]GC[CT]^C BmyI 117 GAGCCC G[AGT]GC[ACT]^C BsaHI 238 GGCGTC G[GA]^CG[CT]C BsaWI 60 ACCGGT [AT]^CCGG[AT] BsgI 64 GTGCAG GTGCAG(16/14) BsiYI 24 CCTGCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 24 CCTGCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 410 GTCTC GTCTC(1/5) BsmFI 241 GTCCC GGGAC(10/14) Bsp1286I 117 GAGCCC G[AGT]GC[ACT]^C BsrFI 60 ACCGGT [GA]^CCGG[CT] Cac8I 140 GCCAGC GC[ACGT]^[ACGT]GC CfoI 34 GCGC GCG^C Cfr10I 60 ACCGGT [GA]^CCGG[CT] DpnII 438 GATC ^GATC DsaI 215 CCGTGG C^C[GA][CT]GG EarI 412 CTCTTC CTCTTC(1/4) HaeII 33 GGCGCT [GA]GCGC^[CT] HapII 61 CCGG C^CGG HhaI 34 GCGC GCG^C Hin1I 238 GGCGTC G[GA]^CG[CT]C HinP1I 34 GCGC G^CGC HpaII 61 CCGG C^CGG Ksp632I 412 CTCTTC CTCTTC(1/4) MaeIII 175 GTCAC ^GT[ACGT]AC MboI 438 GATC ^GATC MboII 413 TCTTC GAAGA(8/7) MnlI 359 CCTC CCTC(7/6) MseI 290 TTAA T^TAA MslI 228 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 61 CCGG C^CGG NdeII 438 GATC ^GATC PinAI 60 ACCGGT A^CCGGT PvuII 142 CAGCTG CAG^CTG RsaI 433 GTAC GT^AC Sau3AI 438 GATC ^GATC SfaNI 227 GCATC GCATC(5/9) SfcI 318 CTATAG C^T[GA][CT]AG SspI 190 AATATT AAT^ATT Tru9I 290 TTAA T^TAA Tsp45I 175 GTCAC ^GT[GC]AC Tsp509I 288 AATT ^AATT TspRI 341 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AciI 44 CCGC CCGC(-3/-1) 80 GCGG BlpI 36 GCTCAGC GC^T[ACGT]AGC 144 GCTGAGC Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC 144 GCTGAGC BsaJI 50 CCCAGG C^C[ACGT][ACGT]GG 215 CCGTGG CelII 36 GCTCAGC GC^T[ACGT]AGC 144 GCTGAGC HaeIII 54 GGCC GG^CC 213 GGCC HgaI 167 GCGTC GACGC(5/10) 239 GCGTC HphI 176 TCACC GGTGA(8/7) 182 TCACC MspA1I 44 CCGCTG C[AC]G^C[GT]G 142 CAGCTG NlaIII 122 CATG CATG^ 223 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BbvI BsrI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI ItaI MvaI MwoI ScrFI TseI