################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbrPI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SfiI SfuI SgrAI SnaBI SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 335 GGTACC G^GTACC AccI 625 GTCTAC GT^[AC][GT]AC AccII 271 CGCG CG^CG AcyI 616 GGCGCC G[GA]^CG[CT]C AflIII 347 ACATGT A^C[GA][CT]GT Alw44I 1357 GTGCAC G^TGCAC AocI 611 CCTCAGG CC^T[ACGT]AGG Aor51HI 1487 AGCGCT AGC^GCT ApaLI 1357 GTGCAC G^TGCAC Asp718I 335 GGTACC G^GTACC BamHI 164 GGATCC G^GATCC BbeI 616 GGCGCC GGCGC^C BciVI 717 GGATAC GTATCC(6/5) BglI 1379 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1229 AGATCT A^GATCT BsaAI 1531 TACGTG [CT]AC^GT[GA] BsaHI 616 GGCGCC G[GA]^CG[CT]C BsiEI 1377 CGGCCG CG[GA][CT]^CG BsmBI 367 GAGACG CGTCTC(1/5) BspHI 1049 TCATGA T^CATGA BspLU11I 347 ACATGT A^CATGT BsrBI 487 CCGCTC CCGCTC(-3/-3) BsrFI 1369 GCCGGC [GA]^CCGG[CT] BssSI 651 CACGAG CACGAG(-5/-1) BstUI 271 CGCG CG^CG Bsu36I 611 CCTCAGG CC^T[ACGT]AGG Cfr10I 1369 GCCGGC [GA]^CCGG[CT] DrdI 1261 GACCATGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1377 CGGCCG C^GGCCG EarI 1764 GAAGAG CTCTTC(1/4) EclXI 1377 CGGCCG C^GGCCG Eco47III 1487 AGCGCT AGC^GCT Eco52I 1377 CGGCCG C^GGCCG Eco81I 611 CCTCAGG CC^T[ACGT]AGG Hin1I 616 GGCGCC G[GA]^CG[CT]C KasI 616 GGCGCC G^GCGCC KpnI 335 GGTACC GGTAC^C Ksp632I 1764 GAAGAG CTCTTC(1/4) MvnI 271 CGCG CG^CG NaeI 1369 GCCGGC GCC^GGC NarI 616 GGCGCC GG^CGCC NgoMIV 1369 GCCGGC G^CCGGC PflMI 786 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 934 CAGCTG CAG^CTG RcaI 1049 TCATGA T^CATGA SapI 1764 GAAGAGC GCTCTTC(1/4) ScaI 236 AGTACT AGT^ACT SexAI 572 ACCTGGT A^CC[AT]GGT SfoI 616 GGCGCC GGC^GCC SmaI 895 CCCGGG CCC^GGG SpeI 322 ACTAGT A^CTAGT SphI 1112 GCATGC GCATG^C TfiI 1221 GATTC G^A[AT]TC Van91I 786 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 895 CCCGGG C^CCGGG XmaI 895 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AlwNI 787 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG 1243 CAGTACCTG ApaI 1129 GGGCCC GGGCC^C 1345 GGGCCC AspHI 451 GTGCTC G[AT]GC[AT]^C 1357 GTGCAC BalI 342 TGGCCA TGG^CCA 1535 TGGCCA BbsI 591 GAAGAC GAAGAC(2/6) 1461 GAAGAC BbvCI 420 CCTCAGC CCTCAGC(-5/-2) 1293 CCTCAGC BcnI 895 CCCGG CC^[GC]GG 896 CCGGG BfaI 323 CTAG C^TAG 940 CTAG BpuAI 591 GAAGAC GAAGAC(2/6) 1461 GAAGAC BsgI 1705 CTGCAC GTGCAG(16/14) 1501 GTGCAG BsiHKAI 451 GTGCTC G[AT]GC[AT]^C 1357 GTGCAC BsmI 660 GAATGC GAATGC(1/-1) 1644 GAATGC BspMI 629 ACCTGC ACCTGC(4/8) 1703 ACCTGC BstEII 150 GGTGACC G^GT[ACGT]ACC 495 GGTCACC BstPI 150 GGTGACC G^GT[ACGT]ACC 495 GGTCACC BstYI 164 GGATCC [GA]^GATC[CT] 1229 AGATCT EcoNI 54 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1336 CCTCACGCAGG EcoO65I 150 GGTGACC G^GT[ACGT]ACC 495 GGTCACC MaeI 323 CTAG C^TAG 940 CTAG MaeII 400 ACGT A^CGT 1532 ACGT MflI 164 GGATCC [GA]^GATC[CT] 1229 AGATCT MluNI 342 TGGCCA TGG^CCA 1535 TGGCCA MscI 342 TGGCCA TGG^CCA 1535 TGGCCA NciI 895 CCCGG CC^[GC]GG 896 CCGGG NcoI 339 CCATGG C^CATGG 1263 CCATGG PpuMI 1268 GGGTCCC [GA]G^G[AT]CC[CT] 1577 AGGTCCT SbfI 261 CCTGCAGG CCTGCA^GG 870 CCTGCAGG SfaNI 43 GATGC GCATC(5/9) 1373 GCATC Sse8387I 261 CCTGCAGG CCTGCA^GG 870 CCTGCAGG StuI 608 AGGCCT AGG^CCT 1658 AGGCCT TaqI 557 TCGA T^CGA 1403 TCGA TthHB8I 557 TCGA T^CGA 1403 TCGA XhoII 164 GGATCC [GA]^GATC[CT] 1229 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BanI BanII BbvI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TseI Tsp45I TspRI XcmI