Restriction map for
pFN21AE6242
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AvrII BbeI BbrPI BbsI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HinfI HpaI KasI
KspI MamI MfeI MluI MroI
MslI MunI NarI NciI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 349 GGTACC G^GTACC
AcsI 339 AAATTT [GA]^AATT[CT]
AcyI 598 GGCGTC G[GA]^CG[CT]C
Aor51HI 21 AGCGCT AGC^GCT
ApoI 339 AAATTT [GA]^AATT[CT]
Asp718I 349 GGTACC G^GTACC
AviII 230 TGCGCA TGC^GCA
BalI 56 TGGCCA TGG^CCA
BamHI 617 GGATCC G^GATCC
BanI 349 GGTACC G^G[CT][GA]CC
BbvCI 781 GCTGAGG CCTCAGC(-5/-2)
BcgI 355 CGAGATGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 531 GGATAC GTATCC(6/5)
BfaI 416 CTAG C^TAG
BpmI 32 CTGGAG CTGGAG(16/14)
BsaHI 598 GGCGTC G[GA]^CG[CT]C
BsgI 740 GTGCAG GTGCAG(16/14)
BsiEI 134 CGGCCG CG[GA][CT]^CG
BsmI 669 GAATGC GAATGC(1/-1)
BsrFI 77 GCCGGC [GA]^CCGG[CT]
BsrI 31 ACTGG ACTGG(1/-1)
Cfr10I 77 GCCGGC [GA]^CCGG[CT]
DrdI 253 GACGTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 792 CCATGG C^C[GA][CT]GG
EagI 134 CGGCCG C^GGCCG
EarI 813 GAAGAG CTCTTC(1/4)
EclXI 134 CGGCCG C^GGCCG
Eco47III 21 AGCGCT AGC^GCT
Eco52I 134 CGGCCG C^GGCCG
EcoNI 228 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 230 TGCGCA TGC^GCA
HaeII 21 AGCGCT [GA]GCGC^[CT]
Hin1I 598 GGCGTC G[GA]^CG[CT]C
HincII 262 GTCAAC GT[CT]^[GA]AC
HindII 262 GTCAAC GT[CT]^[GA]AC
KpnI 349 GGTACC GGTAC^C
Ksp632I 813 GAAGAG CTCTTC(1/4)
MaeI 416 CTAG C^TAG
MluNI 56 TGGCCA TGG^CCA
MscI 56 TGGCCA TGG^CCA
MseI 440 TTAA T^TAA
NaeI 77 GCCGGC GCC^GGC
NcoI 792 CCATGG C^CATGG
NgoMIV 77 GCCGGC G^CCGGC
PflMI 177 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
TaqI 191 TCGA T^CGA
Tru9I 440 TTAA T^TAA
TthHB8I 191 TCGA T^CGA
Van91I 177 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 363 GTGCAC G^TGCAC
547 GTGCAC
AlwNI 142 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
776 CAGATGCTG
ApaLI 363 GTGCAC G^TGCAC
547 GTGCAC
AvaI 87 CTCGGG C^[CT]CG[GA]G
353 CCCGAG
AvaII 709 GGACC G^G[AT]CC
787 GGTCC
BsaI 481 GAGACC GGTCTC(1/5)
745 GAGACC
BseRI 493 GAGGAG GAGGAG(10/8)
496 GAGGAG
BsmAI 481 GAGAC GTCTC(1/5)
745 GAGAC
BsoBI 87 CTCGGG C^[CT]CG[GA]G
353 CCCGAG
BspHI 422 TCATGA T^CATGA
512 TCATGA
BstF5I 12 CATCC GGATG(2/0)
207 CATCC
BstYI 617 GGATCC [GA]^GATC[CT]
659 AGATCC
EaeI 56 TGGCCA [CT]^GGCC[GA]
134 CGGCCG
FokI 12 CATCC GGATG(9/13)
207 CATCC
HapII 8 CCGG C^CGG
78 CCGG
HpaII 8 CCGG C^CGG
78 CCGG
MflI 617 GGATCC [GA]^GATC[CT]
659 AGATCC
MspA1I 145 CAGCTG C[AC]G^C[GT]G
181 CAGCTG
MspI 8 CCGG C^CGG
78 CCGG
PpuMI 708 GGGACCC [GA]G^G[AT]CC[CT]
786 GGGTCCC
PvuII 145 CAGCTG CAG^CTG
181 CAGCTG
RcaI 422 TCATGA T^CATGA
512 TCATGA
XhoII 617 GGATCC [GA]^GATC[CT]
659 AGATCC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HgaI HhaI HinP1I HphI ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I Tsp509I TspRI