(Circular) (Six-base) MAPSORT of: pFN21AE6242.seq Check: 7020 from: 1 to: 5536 pFN21AE6242 5536 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3346 278 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2879 1937 Size: 942 4594 Acc65I G'GTAC_C Cuts at: 2354 2857 2354 Size: 503 5033 AclI AA'CG_TT Cuts at: 1563 3663 4036 1563 Size: 2100 373 3063 Fragments arranged by size: 3063 2100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 3599 4983 5251 1632 Size: 714 1253 1384 268 1917 Fragments arranged by size: 1917 1384 1253 714 268 AfeI AGC'GCT Cuts at: 2028 2028 Size: 5536 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5339 AflIII A'CryG_T Cuts at: 1203 4435 5412 1203 Size: 3232 977 1327 Fragments arranged by size: 3232 1327 977 AgeI A'CCGG_T Cuts at: 4367 4367 Size: 5536 AhdI GACnn_n'nnGTC Cuts at: 4265 4265 Size: 5536 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5536 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5536 AlwNI CAG_nnn'CTG Cuts at: 2152 2786 3279 4851 2152 Size: 634 493 1572 2837 Fragments arranged by size: 2837 1572 634 493 ApaLI G'TGCA_C Cuts at: 2368 2552 3593 4749 2368 Size: 184 1041 1156 3155 Fragments arranged by size: 3155 1156 1041 184 ApoI r'AATT_y Cuts at: 1493 2344 2845 3071 3223 5332 1493 Size: 851 501 226 152 2109 1697 Fragments arranged by size: 2109 1697 851 501 226 152 AseI AT'TA_AT Cuts at: 160 1032 4090 160 Size: 872 3058 1606 Fragments arranged by size: 3058 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2358 2861 1870 Size: 73 149 266 503 4545 Fragments arranged by size: 4545 503 266 149 73 BamHI G'GATC_C Cuts at: 2622 2866 3233 2622 Size: 244 367 4925 Fragments arranged by size: 4925 367 244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 2857 4312 618 Size: 325 217 627 567 503 1455 1842 Fragments arranged by size: 1842 1455 627 567 503 325 217 BanII G_rGCy'C Cuts at: 1391 2174 2443 2517 2735 2855 1391 Size: 783 269 74 218 120 4072 Fragments arranged by size: 4072 783 269 218 120 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4565 BbvCI CC'TCA_GC Cuts at: 2787 2787 Size: 5536 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2349 2383 3727 3761 1903 Size: 34 412 34 1344 34 3678 Fragments arranged by size: 3678 1344 412 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2530 3428 4638 2530 Size: 898 1210 3428 Fragments arranged by size: 3428 1210 898 BclI T'GATC_A Cuts at: 1548 1548 Size: 5536 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4147 136 Size: 107 122 71 631 605 2475 1525 Fragments arranged by size: 2475 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5531 5531 Size: 5536 BlpI GC'TnA_GC Cuts at: 2953 2953 Size: 5536 Bme1580I G_kGCm'C Cuts at: 1790 2372 2556 3597 4753 1790 Size: 582 184 1041 1156 2573 Fragments arranged by size: 2573 1156 1041 582 184 BmgBI CAC'GTC Cuts at: 1318 2101 2122 2260 1318 Size: 783 21 138 4594 Fragments arranged by size: 4594 783 138 21 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3267 4225 442 Size: 416 53 83 399 24 1850 958 1753 Fragments arranged by size: 1850 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5536 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 4196 1239 Size: 819 2138 2579 Fragments arranged by size: 2579 2138 819 Bpu10I CC'TnA_GC Cuts at: 2168 2787 2168 Size: 619 4917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3661 4526 4824 3661 Size: 865 298 4373 Fragments arranged by size: 4373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2480 2744 4199 882 Size: 617 981 264 1455 2219 Fragments arranged by size: 2219 1455 981 617 264 BsaAI yAC'GTr Cuts at: 493 1204 3327 493 Size: 711 2123 2702 Fragments arranged by size: 2702 2123 711 BsaBI GATnn'nnATC Cuts at: 5530 5530 Size: 5536 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2604 3343 3725 4360 Size: 53 83 186 936 1071 739 382 635 Cuts at: 4360 275 Size: 1451 Fragments arranged by size: 1451 1071 936 739 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3969 4367 4641 4788 5199 3969 Size: 398 274 147 411 4306 Fragments arranged by size: 4306 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5311 Fragments arranged by size: 5311 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2516 1186 Size: 1327 3 4206 Fragments arranged by size: 4206 1327 3 BseYI C'CCAG_C Cuts at: 2573 4739 5235 2573 Size: 2166 496 2874 Fragments arranged by size: 2874 2166 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2766 1163 Size: 573 1030 3933 Fragments arranged by size: 3933 1030 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 2986 3747 4775 1540 Size: 275 186 141 844 761 1028 2301 Fragments arranged by size: 2301 1028 844 761 275 186 141 BsiHKAI G_wGCw'C Cuts at: 2109 2372 2556 3597 3682 4753 2109 Size: 263 184 1041 85 1071 2892 Fragments arranged by size: 2892 1071 1041 263 184 85 BsmI GAATG_Cn' Cuts at: 2680 3047 3140 2680 Size: 367 93 5076 Fragments arranged by size: 5076 367 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2174 2372 2443 2517 2556 2735 Size: 399 319 65 198 71 74 39 179 Cuts at: 2735 2855 3597 3682 4753 1391 Size: 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 319 198 179 120 85 74 71 65 39 BspEI T'CCGG_A Cuts at: 5199 5199 Size: 5536 BspHI T'CATG_A Cuts at: 2427 2517 3425 5155 2427 Size: 90 908 1730 2808 Fragments arranged by size: 2808 1730 908 90 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2891 844 Size: 2047 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3423 1394 Size: 429 1600 3507 Fragments arranged by size: 3507 1600 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4031 4205 65 Size: 1183 2783 174 1396 Fragments arranged by size: 2783 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2082 4180 4367 1700 Size: 110 272 2098 187 2869 Fragments arranged by size: 2869 2098 272 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5536 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5536 BssSI C'ACGA_G Cuts at: 3596 4608 3596 Size: 1012 4524 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5536 BstBI TT'CG_AA Cuts at: 4340 4340 Size: 5536 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5536 BstYI r'GATC_y Cuts at: 1972 2622 2664 2866 3233 3618 3635 5087 5173 Size: 650 42 202 367 385 17 1452 86 Cuts at: 5173 5185 5531 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 650 385 367 346 202 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2797 513 Size: 552 1732 3252 Fragments arranged by size: 3252 1732 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2266 487 Size: 683 56 1040 3757 Fragments arranged by size: 3757 1040 683 56 BtsI GCAGTG_nn' Cuts at: 2488 3052 3846 3866 2488 Size: 564 794 20 4158 Fragments arranged by size: 4158 794 564 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5536 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5501 DraI TTT'AAA Cuts at: 2840 2840 Size: 5536 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2264 4543 809 Size: 507 948 2279 1802 Fragments arranged by size: 2279 1802 948 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2139 2983 Size: 54 1425 50 275 18 231 78 844 Cuts at: 2983 3872 5501 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 844 275 231 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2139 2983 1537 Size: 275 327 844 4090 Fragments arranged by size: 4090 844 327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2813 3466 1353 Size: 1460 653 3423 Fragments arranged by size: 3423 1460 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 4123 4497 4643 387 Size: 1064 138 585 1949 374 146 1280 Fragments arranged by size: 1949 1280 1064 585 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2346 3599 4196 4983 5251 1239 Size: 393 426 288 1253 597 787 268 1524 Fragments arranged by size: 1524 1253 787 597 426 393 288 268 EcoNI CCTnn'n_nnAGG Cuts at: 2237 2237 Size: 5536 EcoO109I rG'GnC_Cy Cuts at: 2164 2251 2714 2792 2164 Size: 87 463 78 4908 Fragments arranged by size: 4908 463 87 78 EcoRI G'AATT_C Cuts at: 2845 3223 2845 Size: 378 5158 FspI TGC'GCA Cuts at: 2237 4042 2237 Size: 1805 3731 HaeII r_GCGC'y Cuts at: 1678 2030 4683 1678 Size: 352 2653 2531 Fragments arranged by size: 2653 2531 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373 Size: 32 135 32 2605 32 42 32 2626 Fragments arranged by size: 2626 2605 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2269 2880 3126 669 Size: 988 281 331 611 246 3079 Fragments arranged by size: 3079 988 611 331 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5536 HpaI GTT'AAC Cuts at: 3126 3126 Size: 5536 KpnI G_GTAC'C Cuts at: 2358 2861 2358 Size: 503 5033 MfeI C'AATT_G Cuts at: 3135 3135 Size: 5536 MluI A'CGCG_T Cuts at: 5412 5412 Size: 5536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4650 4834 1305 Size: 3345 184 2007 Fragments arranged by size: 3345 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 10 Size: 54 1425 343 231 3483 Fragments arranged by size: 3483 1425 343 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 3494 3853 4012 518 Size: 648 2328 359 159 2042 Fragments arranged by size: 2328 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2152 2188 2952 3629 4777 5022 1606 Size: 546 36 764 677 1148 245 2120 Fragments arranged by size: 2120 1148 764 677 546 245 36 NaeI GCC'GGC Cuts at: 2084 2084 Size: 5536 NcoI C'CATG_G Cuts at: 513 1065 2797 513 Size: 552 1732 3252 Fragments arranged by size: 3252 1732 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5536 NgoMIV G'CCGG_C Cuts at: 2082 2082 Size: 5536 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5536 NotI GC'GGCC_GC Cuts at: 2983 2983 Size: 5536 NspI r_CATG'y Cuts at: 1132 1229 2894 4439 1132 Size: 97 1665 1545 2229 Fragments arranged by size: 2229 1665 1545 97 PasI CC'CwG_GG Cuts at: 1348 2687 1348 Size: 1339 4197 PciI A'CATG_T Cuts at: 4435 4435 Size: 5536 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 1108 Size: 1080 4456 PfoI T'CCnGG_A Cuts at: 2618 2618 Size: 5536 PmeI GTTT'AAAC Cuts at: 2840 2840 Size: 5536 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3605 3637 5118 5150 3605 Size: 32 1481 32 3991 Fragments arranged by size: 3991 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2714 2792 2714 Size: 78 5458 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5536 PsiI TTA'TAA Cuts at: 3106 3106 Size: 5536 PstI C_TGCA'G Cuts at: 830 2157 2208 2456 2469 2888 830 Size: 1327 51 248 13 419 3478 Fragments arranged by size: 3478 1327 419 248 51 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5536 PvuII CAG'CTG Cuts at: 2152 2188 2152 Size: 36 5500 SalI G'TCGA_C Cuts at: 1936 2878 1936 Size: 942 4594 SanDI GG'GwC_CC Cuts at: 2714 2792 2714 Size: 78 5458 SbfI CC_TGCA'GG Cuts at: 2888 2888 Size: 5536 ScaI AGT'ACT Cuts at: 3784 5496 3784 Size: 1712 3824 SfcI C'TryA_G Cuts at: 826 1045 2153 2204 2452 2465 2884 4019 4700 Size: 219 1108 51 248 13 419 1135 681 Cuts at: 4700 4891 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1108 681 419 248 219 191 51 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5536 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5536 SmaI CCC'GGG Cuts at: 1872 2863 1872 Size: 991 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3640 4541 4803 820 Size: 197 926 1697 901 262 1553 Fragments arranged by size: 1697 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3327 493 Size: 2834 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5536 SphI G_CATG'C Cuts at: 1132 2894 1132 Size: 1762 3774 SspI AAT'ATT Cuts at: 5 52 3460 5 Size: 47 3408 2081 Fragments arranged by size: 3408 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5536 StyI C'CwwG_G Cuts at: 513 1065 2398 2718 2797 2975 513 Size: 552 1333 320 79 178 3074 Fragments arranged by size: 3074 1333 552 320 178 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2683 3563 3580 3733 3918 4363 325 Size: 1255 1103 880 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1103 880 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2760 3782 5494 Size: 274 80 33 51 1443 783 1022 1712 Cuts at: 5494 96 Size: 138 Fragments arranged by size: 1712 1443 1022 783 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2626 2658 5496 5528 2626 Size: 32 2838 32 2634 Fragments arranged by size: 2838 2634 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5536 XbaI T'CTAG_A Cuts at: 2872 2872 Size: 5536 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5536 XmaI C'CCGG_G Cuts at: 1870 2861 1870 Size: 991 4545 XmnI GAAnn'nnTTC Cuts at: 3665 3665 Size: 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3344 276 Size: 53 83 186 936 1810 2468 Fragments arranged by size: 2468 1810 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI