Restriction map for
pFN21AE6242
(Linear) (Six-base) MAPSORT of: KIEE6242.seq Check: 3672 from: 1 to: 831
KIEE6242.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
Acc65I G'GTAC_C
Cuts at: 0 349 831
Size: 349 482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 831
Size: 341 490
AfeI AGC'GCT
Cuts at: 0 23 831
Size: 23 808
AlwNI CAG_nnn'CTG
Cuts at: 0 147 781 831
Size: 147 634 50
Fragments arranged by size:
634 147 50
ApaLI G'TGCA_C
Cuts at: 0 363 547 831
Size: 363 184 284
Fragments arranged by size:
363 284 184
ApoI r'AATT_y
Cuts at: 0 339 831
Size: 339 492
AvaI C'yCGr_G
Cuts at: 0 87 353 831
Size: 87 266 478
Fragments arranged by size:
478 266 87
BamHI G'GATC_C
Cuts at: 0 617 831
Size: 617 214
BanI G'GyrC_C
Cuts at: 0 349 831
Size: 349 482
BanII G_rGCy'C
Cuts at: 0 169 438 512 730 831
Size: 169 269 74 218 101
Fragments arranged by size:
269 218 169 101 74
BbvCI CC'TCA_GC
Cuts at: 0 782 831
Size: 782 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 344 378 831
Size: 344 34 453
Fragments arranged by size:
453 344 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 525 831
Size: 525 306
Bme1580I G_kGCm'C
Cuts at: 0 367 551 831
Size: 367 184 280
Fragments arranged by size:
367 280 184
BmgBI CAC'GTC
Cuts at: 0 96 117 255 831
Size: 96 21 138 576
Fragments arranged by size:
576 138 96 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 831
Size: 53 778
Bpu10I CC'TnA_GC
Cuts at: 0 163 782 831
Size: 163 619 49
Fragments arranged by size:
619 163 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 475 739 831
Size: 475 264 92
Fragments arranged by size:
475 264 92
BsaHI Gr'CG_yC
Cuts at: 0 599 831
Size: 599 232
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 511 831
Size: 508 3 320
Fragments arranged by size:
508 320 3
BseYI C'CCAG_C
Cuts at: 0 568 831
Size: 568 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 831
Size: 761 70
BsiEI CG_ry'CG
Cuts at: 0 137 831
Size: 137 694
BsiHKAI G_wGCw'C
Cuts at: 0 104 367 551 831
Size: 104 263 184 280
Fragments arranged by size:
280 263 184 104
BsmI GAATG_Cn'
Cuts at: 0 675 831
Size: 675 156
Bsp1286I G_dGCh'C
Cuts at: 0 104 169 367 438 512 551 730 831
Size: 104 65 198 71 74 39 179 101
Fragments arranged by size:
198 179 104 101 74 71 65 39
BspHI T'CATG_A
Cuts at: 0 422 512 831
Size: 422 90 319
Fragments arranged by size:
422 319 90
BsrFI r'CCGG_y
Cuts at: 0 77 831
Size: 77 754
BstYI r'GATC_y
Cuts at: 0 617 659 831
Size: 617 42 172
Fragments arranged by size:
617 172 42
BtgI C'CryG_G
Cuts at: 0 792 831
Size: 792 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 261 831
Size: 261 570
BtsI GCAGTG_nn'
Cuts at: 0 483 831
Size: 483 348
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 831
Size: 259 572
EaeI y'GGCC_r
Cuts at: 0 56 134 831
Size: 56 78 697
Fragments arranged by size:
697 78 56
EagI C'GGCC_G
Cuts at: 0 134 831
Size: 134 697
EarI CTCTTCn'nnn_
Cuts at: 0 808 831
Size: 808 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 169 831
Size: 169 662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 341 831
Size: 53 288 490
Fragments arranged by size:
490 288 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 831
Size: 232 599
EcoO109I rG'GnC_Cy
Cuts at: 0 159 246 709 787 831
Size: 159 87 463 78 44
Fragments arranged by size:
463 159 87 78 44
FspI TGC'GCA
Cuts at: 0 232 831
Size: 232 599
HaeII r_GCGC'y
Cuts at: 0 25 831
Size: 25 806
HincII GTy'rAC
Cuts at: 0 264 831
Size: 264 567
KpnI G_GTAC'C
Cuts at: 0 353 831
Size: 353 478
MscI TGG'CCA
Cuts at: 0 58 831
Size: 58 773
MspA1I CmG'CkG
Cuts at: 0 147 183 831
Size: 147 36 648
Fragments arranged by size:
648 147 36
NaeI GCC'GGC
Cuts at: 0 79 831
Size: 79 752
NcoI C'CATG_G
Cuts at: 0 792 831
Size: 792 39
NgoMIV G'CCGG_C
Cuts at: 0 77 831
Size: 77 754
PasI CC'CwG_GG
Cuts at: 0 682 831
Size: 682 149
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 831
Size: 183 648
PfoI T'CCnGG_A
Cuts at: 0 613 831
Size: 613 218
PpuMI rG'GwC_Cy
Cuts at: 0 709 787 831
Size: 709 78 44
Fragments arranged by size:
709 78 44
PstI C_TGCA'G
Cuts at: 0 152 203 451 464 831
Size: 152 51 248 13 367
Fragments arranged by size:
367 248 152 51 13
PvuII CAG'CTG
Cuts at: 0 147 183 831
Size: 147 36 648
Fragments arranged by size:
648 147 36
SanDI GG'GwC_CC
Cuts at: 0 709 787 831
Size: 709 78 44
Fragments arranged by size:
709 78 44
SfcI C'TryA_G
Cuts at: 0 148 199 447 460 831
Size: 148 51 248 13 371
Fragments arranged by size:
371 248 148 51 13
StyI C'CwwG_G
Cuts at: 0 393 713 792 831
Size: 393 320 79 39
Fragments arranged by size:
393 320 79 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 678 831
Size: 678 153
TatI w'GTAC_w
Cuts at: 0 755 831
Size: 755 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 621 653 831
Size: 621 32 178
Fragments arranged by size:
621 178 32
Enzymes that do cut:
Acc65I AcuI AfeI AlwNI ApaLI ApoI AvaI BamHI
BanI BanII BbvCI BcgI BciVI Bme1580I BmgBI BpmI
Bpu10I BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrFI BstYI BtgI BtgZI BtsI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII HincII KpnI MscI MspA1I NaeI NcoI
NgoMIV PasI PflMI PfoI PpuMI PstI PvuII SanDI
SfcI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BclI BfrBI BglI BglII BlpI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI
BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII EcoICRI EcoRI EcoRV FalI FseI
FspAI Hin4I HindIII HpaI KasI MfeI MluI MmeI
MslI NarI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI