################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII HphI KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 507 GTAC GT^AC AlwI 348 GATCC GGATC(4/5) AlwNI 124 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 323 GAGCAC G[AT]GC[AT]^C BbsI 442 GTCTTC GAAGAC(2/6) BciVI 78 GTATCC GTATCC(6/5) BpuAI 442 GTCTTC GAAGAC(2/6) BsaJI 47 CCATGG C^C[ACGT][ACGT]GG BsiHKAI 323 GAGCAC G[AT]GC[AT]^C BsmFI 234 GTCCC GGGAC(10/14) BssKI 166 CCAGG ^CC[ACGT]GG BssSI 320 CACGAG CACGAG(-5/-1) BstNI 166 CCAGG CC^[AT]GG BstXI 41 CCACATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 347 AGATCC [GA]^GATC[CT] DraI 544 TTTAAA TTT^AAA DsaI 47 CCATGG C^C[GA][CT]GG EcoRI 295 GAATTC G^AATTC EcoRII 166 CCAGG ^CC[AT]GG EcoT14I 47 CCATGG C^C[AT][AT]GG HaeIII 633 GGCC GG^CC HincII 220 GTTAAC GT[CT]^[GA]AC HindII 220 GTTAAC GT[CT]^[GA]AC HinfI 232 GAGTC G^A[ACGT]TC HpaI 220 GTTAAC GTT^AAC MaeII 538 ACGT A^CGT MflI 347 AGATCC [GA]^GATC[CT] MspA1I 251 CAGCTG C[AC]G^C[GT]G MvaI 166 CCAGG CC^[AT]GG NcoI 47 CCATGG C^CATGG PleI 232 GAGTC GAGTC(4/5) PvuII 251 CAGCTG CAG^CTG RsaI 507 GTAC GT^AC ScaI 506 AGTACT AGT^ACT ScrFI 166 CCAGG CC^[ACGT]GG StyI 47 CCATGG C^C[AT][AT]GG XhoII 347 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 137 CCGC CCGC(-3/-1) 468 CCGC BbvI 118 GCAGC GCAGC(8/12) 250 GCAGC BfaI 97 CTAG C^TAG 404 CTAG BmyI 323 GAGCAC G[AGT]GC[ACT]^C 473 GGGCAC BsaI 37 GAGACC GGTCTC(1/5) 169 GGTCTC BsmAI 37 GAGAC GTCTC(1/5) 170 GTCTC Bsp1286I 323 GAGCAC G[AGT]GC[ACT]^C 473 GGGCAC BstF5I 44 CATCC GGATG(2/0) 5 GGATG DpnII 348 GATC ^GATC 360 GATC EarI 16 GAAGAG CTCTTC(1/4) 265 GAAGAG FokI 44 CATCC GGATG(9/13) 5 GGATG Ksp632I 16 GAAGAG CTCTTC(1/4) 265 GAAGAG MaeI 97 CTAG C^TAG 404 CTAG MboI 348 GATC ^GATC 360 GATC MslI 42 CACATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 183 CATCATTATG NdeII 348 GATC ^GATC 360 GATC NlaIII 48 CATG CATG^ 75 CATG PstI 116 CTGCAG CTGCA^G 382 CTGCAG Sau3AI 348 GATC ^GATC 360 GATC SfcI 116 CTGCAG C^T[GA][CT]AG 382 CTGCAG TseI 118 GCAGC G^C[AT]GC 250 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AcsI AluI ApoI DdeI Fnu4HI ItaI MboII MnlI MseI Tru9I Tsp509I TspRI