(Circular) (Six-base) MAPSORT of: pFN21AE1303.seq Check: 4989 from: 1 to: 5953 pFN21AE1303 5953 bp With 182 enzymes: SgfI * May 22, 2017 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2056 2742 2056 Size: 686 5267 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2754 3296 1937 Size: 817 542 4594 Fragments arranged by size: 4594 817 542 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 3156 4016 5400 5668 1632 Size: 1302 222 860 1384 268 1917 Fragments arranged by size: 1917 1384 1302 860 268 222 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 4852 5829 1203 Size: 3649 977 1327 Fragments arranged by size: 3649 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 3145 4682 3145 Size: 1537 4416 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AlwNI CAG_nnn'CTG Cuts at: 2147 2750 3696 5268 2147 Size: 603 946 1572 2832 Fragments arranged by size: 2832 1572 946 603 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 3262 3488 3640 5749 1493 Size: 1769 226 152 2109 1697 Fragments arranged by size: 2109 1769 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2939 3278 1870 Size: 73 253 743 339 4545 Fragments arranged by size: 4545 743 339 253 73 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 3274 4729 618 Size: 325 217 627 250 1237 1455 1842 Fragments arranged by size: 1842 1455 1237 627 325 250 217 BanII G_rGCy'C Cuts at: 1391 2481 2535 3130 3272 1391 Size: 1090 54 595 142 4072 Fragments arranged by size: 4072 1090 595 142 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 3144 928 Size: 971 1023 222 3737 Fragments arranged by size: 3737 1023 971 222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2280 2314 2609 2643 2930 2964 4144 Size: 34 343 34 295 34 287 34 1180 Cuts at: 4144 4178 1903 Size: 34 3678 Fragments arranged by size: 3678 1180 343 295 287 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 2178 1548 Size: 630 5323 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2185 5948 2185 Size: 3763 2190 BlpI GC'TnA_GC Cuts at: 2093 3370 2093 Size: 1277 4676 Bme1580I G_kGCm'C Cuts at: 1790 4014 5170 1790 Size: 2224 1156 2573 Fragments arranged by size: 2573 2224 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5953 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2321 3012 3684 Size: 416 53 83 399 24 904 691 672 Cuts at: 3684 4642 442 Size: 958 1753 Fragments arranged by size: 1753 958 904 691 672 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2226 2917 2226 Size: 691 5262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2424 2675 3085 4613 1239 Size: 971 214 251 410 1528 2579 Fragments arranged by size: 2579 1528 971 410 251 214 Bpu10I CC'TnA_GC Cuts at: 2468 3158 2468 Size: 690 5263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2450 4078 4943 5241 2450 Size: 1628 865 298 3162 Fragments arranged by size: 3162 1628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3092 4616 882 Size: 617 1593 1524 2219 Fragments arranged by size: 2219 1593 1524 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2578 2994 3760 Size: 53 83 186 936 552 493 416 766 Cuts at: 3760 4142 4777 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 766 635 552 493 416 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2371 3225 4386 4784 5058 5205 5616 2371 Size: 854 1161 398 274 147 411 2708 Fragments arranged by size: 2708 1161 854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5728 Fragments arranged by size: 5728 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2578 1186 Size: 1392 4561 BseYI C'CCAG_C Cuts at: 2822 5156 5652 2822 Size: 2334 496 3123 Fragments arranged by size: 3123 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5380 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2784 3403 4164 5192 1540 Size: 275 186 783 619 761 1028 2301 Fragments arranged by size: 2301 1028 783 761 619 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 2428 2683 4014 4099 5170 2337 Size: 91 255 1331 85 1071 3120 Fragments arranged by size: 3120 1331 1071 255 91 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2428 2481 2535 2683 3130 3272 Size: 399 547 91 53 54 148 447 142 Cuts at: 3272 4014 4099 5170 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 547 447 399 148 142 91 85 54 53 BspEI T'CCGG_A Cuts at: 3225 5616 3225 Size: 2391 3562 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2056 2742 3150 3308 844 Size: 1212 686 408 158 3489 Fragments arranged by size: 3489 1212 686 408 158 BsrBI CCG'CTC Cuts at: 1394 1823 3840 1394 Size: 429 2017 3507 Fragments arranged by size: 3507 2017 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4448 4622 65 Size: 1183 3200 174 1396 Fragments arranged by size: 3200 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 4597 4784 1700 Size: 110 230 2557 187 2869 Fragments arranged by size: 2869 2557 230 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 4013 5025 4013 Size: 1012 4941 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5953 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstXI CCAn_nnnn'nTGG Cuts at: 2389 2638 2864 2389 Size: 249 226 5478 Fragments arranged by size: 5478 249 226 BstYI r'GATC_y Cuts at: 1972 2150 2185 3283 3650 4035 4052 5504 5590 Size: 178 35 1098 367 385 17 1452 86 Cuts at: 5590 5602 5948 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1098 385 367 346 178 86 35 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2674 2884 513 Size: 552 939 670 210 3582 Fragments arranged by size: 3582 939 670 552 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 2488 3128 3469 4263 4283 2488 Size: 640 341 794 20 4158 Fragments arranged by size: 4158 794 640 341 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3244 3257 3244 Size: 13 5940 DraIII CAC_nnn'GTG Cuts at: 3020 3020 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2485 3400 4289 Size: 54 1425 50 275 18 655 915 889 Cuts at: 4289 5918 8 Size: 1629 43 Fragments arranged by size: 1629 1425 915 889 655 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2217 3883 1353 Size: 699 165 1666 3423 Fragments arranged by size: 3423 1666 699 165 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2424 2675 2934 3085 3156 4016 Size: 393 578 214 251 259 151 71 860 Cuts at: 4016 4613 5400 5668 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 860 787 597 578 393 268 259 251 214 151 71 EcoO109I rG'GnC_Cy Cuts at: 2112 2976 3070 2112 Size: 864 94 4995 Fragments arranged by size: 4995 864 94 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 5100 1678 Size: 3422 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2170 2202 2984 3016 3131 Size: 32 135 32 598 32 782 32 115 Cuts at: 3131 3163 4594 4626 4668 4700 1373 Size: 32 1431 32 42 32 2626 Fragments arranged by size: 2626 1431 782 598 135 115 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3297 3543 669 Size: 988 281 1359 246 3079 Fragments arranged by size: 3079 1359 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2577 5067 5251 1305 Size: 1272 2490 184 2007 Fragments arranged by size: 2490 2007 1272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2487 10 Size: 54 1425 343 655 3476 Fragments arranged by size: 3476 1425 655 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3029 3911 4270 4429 518 Size: 648 1863 882 359 159 2042 Fragments arranged by size: 2042 1863 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2176 2817 2989 3369 4046 5194 5439 Size: 531 39 641 172 380 677 1148 245 Cuts at: 5439 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 641 531 380 245 172 39 NaeI GCC'GGC Cuts at: 2042 2042 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2004 2884 513 Size: 552 939 880 3582 Fragments arranged by size: 3582 939 880 552 NdeI CA'TA_TG Cuts at: 387 2118 387 Size: 1731 4222 NgoMIV G'CCGG_C Cuts at: 2040 2040 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2257 3311 4856 1132 Size: 97 1028 1054 1545 2229 Fragments arranged by size: 2229 1545 1054 1028 97 PasI CC'CwG_GG Cuts at: 1348 2744 1348 Size: 1396 4557 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5953 PfoI T'CCnGG_A Cuts at: 2961 3062 2961 Size: 101 5852 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2976 3070 2976 Size: 94 5859 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3195 3523 3195 Size: 328 5625 PspXI vC'TCGA_Gb Cuts at: 2196 2196 Size: 5953 PstI C_TGCA'G Cuts at: 830 2067 2739 3147 3305 830 Size: 1237 672 408 158 3478 Fragments arranged by size: 3478 1237 672 408 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 PvuII CAG'CTG Cuts at: 2176 2817 2989 2176 Size: 641 172 5140 Fragments arranged by size: 5140 641 172 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SanDI GG'GwC_CC Cuts at: 3070 3070 Size: 5953 SapI GCTCTTCn'nnn_ Cuts at: 2052 2217 2052 Size: 165 5788 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 4201 5913 4201 Size: 1712 4241 SfcI C'TryA_G Cuts at: 826 1045 2063 2735 3143 3301 4436 5117 5308 Size: 219 1018 672 408 158 1135 681 191 Cuts at: 5308 826 Size: 1471 Fragments arranged by size: 1471 1135 1018 681 672 408 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2196 2429 4057 4958 5220 820 Size: 197 926 253 233 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 262 253 233 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 2861 1517 Size: 1344 4609 StyI C'CwwG_G Cuts at: 513 1065 2004 2884 3392 513 Size: 552 939 880 508 3074 Fragments arranged by size: 3074 939 880 552 508 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2395 2770 3031 3980 3997 4150 4335 Size: 1255 815 375 261 949 17 153 185 Cuts at: 4335 4780 325 Size: 445 1498 Fragments arranged by size: 1498 1255 949 815 445 375 261 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4199 5911 96 Size: 274 80 33 51 1443 2222 1712 138 Fragments arranged by size: 2222 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2760 2792 5913 5945 2760 Size: 32 3121 32 2768 Fragments arranged by size: 3121 2768 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2864 2864 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 2196 1943 Size: 253 5700 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 2801 4082 2801 Size: 1281 4672 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI