################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BcgI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaWI BseAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cac8I CelII ClaI CpoI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoT22I FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MroI MslI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1159 GACGTC GACGT^C AcyI 1159 GACGTC G[GA]^CG[CT]C AseI 487 ATTAAT AT^TAAT AsnI 487 ATTAAT AT^TAAT Asp700I 1368 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 711 TGGCCA TGG^CCA BbvCI 1458 GCTGAGG CCTCAGC(-5/-2) BciVI 869 GGATAC GTATCC(6/5) BglII 434 AGATCT A^GATCT BpmI 79 CTGGAG CTGGAG(16/14) BsaBI 958 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1159 GACGTC G[GA]^CG[CT]C BsaI 1393 GGTCTC GGTCTC(1/5) BsgI 100 GTGCAG GTGCAG(16/14) BsmFI 1268 GTCCC GGGAC(10/14) Bsp1407I 167 TGTACA T^GTACA BspMI 718 ACCTGC ACCTGC(4/8) BsrFI 44 GCCGGT [GA]^CCGG[CT] BsrGI 167 TGTACA T^GTACA BstAPI 382 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 108 GCGC GCG^C Cfr10I 44 GCCGGT [GA]^CCGG[CT] DraI 1287 TTTAAA TTT^AAA DsaI 509 CCACGG C^C[GA][CT]GG EagI 42 CGGCCG C^GGCCG EclXI 42 CGGCCG C^GGCCG Eco52I 42 CGGCCG C^GGCCG EcoRI 157 GAATTC G^AATTC FauI 18 GCGGG CCCGC(4/6) HhaI 108 GCGC GCG^C Hin1I 1159 GACGTC G[GA]^CG[CT]C HinP1I 108 GCGC G^CGC HindIII 445 AAGCTT A^AGCTT MamI 958 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 711 TGGCCA TGG^CCA MscI 711 TGGCCA TGG^CCA NruI 49 TCGCGA TCG^CGA PshBI 487 ATTAAT AT^TAAT PvuII 76 CAGCTG CAG^CTG ScaI 948 AGTACT AGT^ACT SmlI 214 CTTGAG C^T[CT][GA]AG SspBI 167 TGTACA T^GTACA StuI 281 AGGCCT AGG^CCT TaqI 8 TCGA T^CGA TfiI 219 GAATC G^A[AT]TC TthHB8I 8 TCGA T^CGA XmnI 1368 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 50 CGCG CG^CG 109 CGCG AfaI 168 GTAC GT^AC 949 GTAC BanII 4 GAGCTC G[GA]GC[CT]^C 514 GAGCTC BseRI 1199 GAGGAG GAGGAG(10/8) 1461 GAGGAG BsiEI 42 CGGCCG CG[GA][CT]^CG 46 CGGTCG BsrDI 723 CATTGC GCAATG(2/0) 1154 GCAATG BstUI 50 CGCG CG^CG 109 CGCG BstXI 431 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1211 CCAGTGGGATGG BstYI 434 AGATCT [GA]^GATC[CT] 471 GGATCT DraII 83 AGGCCCT [GA]G^G[ACGT]CC[CT] 1469 AGGCCCT EcoO109I 83 AGGCCCT [GA]G^G[ACGT]CC[CT] 1469 AGGCCCT EcoRV 118 GATATC GAT^ATC 1316 GATATC EcoT14I 192 CCTTGG C^C[AT][AT]GG 974 CCAAGG HgaI 680 GACGC GACGC(5/10) 1266 GCGTC MflI 434 AGATCT [GA]^GATC[CT] 471 GGATCT MspA1I 76 CAGCTG C[AC]G^C[GT]G 1427 CCGCTG MvnI 50 CGCG CG^CG 109 CGCG PleI 612 GACTC GAGTC(4/5) 823 GAGTC PstI 70 CTGCAG CTGCA^G 380 CTGCAG RsaI 168 GTAC GT^AC 949 GTAC SacI 4 GAGCTC GAGCT^C 514 GAGCTC SfcI 70 CTGCAG C^T[GA][CT]AG 380 CTGCAG StyI 192 CCTTGG C^C[AT][AT]GG 974 CCAAGG XhoII 434 AGATCT [GA]^GATC[CT] 471 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AspHI AvaII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI