Restriction map for
pFN21AE4372
(Linear) (Six-base) MAPSORT of: KIEE4372.seq Check: 566 from: 1 to: 1170
KIEE4372.orf
With 182 enzymes: SgfI *
March 22, 2012 17:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 498 1170
Size: 498 672
AflIII A'CryG_T
Cuts at: 0 461 1170
Size: 461 709
AleI CACnn'nnGTG
Cuts at: 0 160 278 1170
Size: 160 118 892
Fragments arranged by size:
892 160 118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 482 1170
Size: 482 688
ApaI G_GGCC'C
Cuts at: 0 836 1170
Size: 836 334
ApoI r'AATT_y
Cuts at: 0 398 917 1170
Size: 398 519 253
Fragments arranged by size:
519 398 253
AvaI C'yCGr_G
Cuts at: 0 377 1170
Size: 377 793
BanI G'GyrC_C
Cuts at: 0 510 1135 1170
Size: 510 625 35
Fragments arranged by size:
625 510 35
BanII G_rGCy'C
Cuts at: 0 185 360 836 1170
Size: 185 175 476 334
Fragments arranged by size:
476 334 185 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 477 511 574 608 1170
Size: 477 34 63 34 562
Fragments arranged by size:
562 477 63 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 355 1170
Size: 355 815
BglII A'GATC_T
Cuts at: 0 820 1170
Size: 820 350
Bme1580I G_kGCm'C
Cuts at: 0 515 836 1170
Size: 515 321 334
Fragments arranged by size:
515 334 321
BmrI ACTGGGnnnn_n'
Cuts at: 0 1044 1170
Size: 1044 126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 418 1170
Size: 418 752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 911 1092 1170
Size: 331 580 181 78
Fragments arranged by size:
580 331 181 78
Bpu10I CC'TnA_GC
Cuts at: 0 1070 1170
Size: 1070 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1170
Size: 943 227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 494 524 1090 1120 1170
Size: 494 30 566 30 50
Fragments arranged by size:
566 494 50 30 30
BseYI C'CCAG_C
Cuts at: 0 523 1089 1170
Size: 523 566 81
Fragments arranged by size:
566 523 81
BsiEI CG_ry'CG
Cuts at: 0 1064 1170
Size: 1064 106
BsiHKAI G_wGCw'C
Cuts at: 0 167 1170
Size: 167 1003
BsmBI CGTCTCn'nnnn_
Cuts at: 0 27 1170
Size: 27 1143
Bsp1286I G_dGCh'C
Cuts at: 0 167 185 360 515 836 1170
Size: 167 18 175 155 321 334
Fragments arranged by size:
334 321 175 167 155 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 498 1170
Size: 498 672
BsrBI CCG'CTC
Cuts at: 0 706 1170
Size: 706 464
BsrDI GCAATG_nn'
Cuts at: 0 495 1170
Size: 495 675
BsrFI r'CCGG_y
Cuts at: 0 54 131 316 1170
Size: 54 77 185 854
Fragments arranged by size:
854 185 77 54
BssSI C'ACGA_G
Cuts at: 0 908 1170
Size: 908 262
BstAPI GCAn_nnn'nTGC
Cuts at: 0 97 1170
Size: 97 1073
BstBI TT'CG_AA
Cuts at: 0 614 1170
Size: 614 556
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1170
Size: 843 327
BstYI r'GATC_y
Cuts at: 0 820 1170
Size: 820 350
BtgI C'CryG_G
Cuts at: 0 867 1058 1170
Size: 867 191 112
Fragments arranged by size:
867 191 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 599 1170
Size: 599 571
ClaI AT'CG_AT
Cuts at: 0 454 1170
Size: 454 716
DrdI GACnn_nn'nnGTC
Cuts at: 0 892 1170
Size: 892 278
EaeI y'GGCC_r
Cuts at: 0 1061 1170
Size: 1061 109
EagI C'GGCC_G
Cuts at: 0 1061 1170
Size: 1061 109
EarI CTCTTCn'nnn_
Cuts at: 0 1079 1170
Size: 1079 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 911 1092 1170
Size: 331 580 181 78
Fragments arranged by size:
580 331 181 78
EcoO109I rG'GnC_Cy
Cuts at: 0 145 381 832 1170
Size: 145 236 451 338
Fragments arranged by size:
451 338 236 145
EcoRV GAT'ATC
Cuts at: 0 774 1170
Size: 774 396
HaeII r_GCGC'y
Cuts at: 0 44 524 1170
Size: 44 480 646
Fragments arranged by size:
646 480 44
HindIII A'AGCT_T
Cuts at: 0 293 617 1170
Size: 293 324 553
Fragments arranged by size:
553 324 293
MslI CAynn'nnrTG
Cuts at: 0 160 278 464 841 1170
Size: 160 118 186 377 329
Fragments arranged by size:
377 329 186 160 118
MspA1I CmG'CkG
Cuts at: 0 240 1170
Size: 240 930
NaeI GCC'GGC
Cuts at: 0 56 318 1170
Size: 56 262 852
Fragments arranged by size:
852 262 56
NgoMIV G'CCGG_C
Cuts at: 0 54 316 1170
Size: 54 262 854
Fragments arranged by size:
854 262 54
NspI r_CATG'y
Cuts at: 0 465 1170
Size: 465 705
PasI CC'CwG_GG
Cuts at: 0 516 1170
Size: 516 654
PciI A'CATG_T
Cuts at: 0 461 1170
Size: 461 709
PflMI CCAn_nnn'nTGG
Cuts at: 0 1145 1170
Size: 1145 25
PspOMI G'GGCC_C
Cuts at: 0 832 1170
Size: 832 338
PstI C_TGCA'G
Cuts at: 0 854 1170
Size: 854 316
PvuII CAG'CTG
Cuts at: 0 240 1170
Size: 240 930
SapI GCTCTTCn'nnn_
Cuts at: 0 1079 1170
Size: 1079 91
SfcI C'TryA_G
Cuts at: 0 192 850 1170
Size: 192 658 320
Fragments arranged by size:
658 320 192
SmaI CCC'GGG
Cuts at: 0 379 1170
Size: 379 791
SmlI C'TyrA_G
Cuts at: 0 958 1170
Size: 958 212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 494 526 763 795 1050 1082 1090 1122
Size: 494 32 237 32 255 32 8 32
Cuts at: 1122 1170
Size: 48
Fragments arranged by size:
494 255 237 48 32 32 32 32
8
XmaI C'CCGG_G
Cuts at: 0 377 1170
Size: 377 793
XmnI GAAnn'nnTTC
Cuts at: 0 917 1170
Size: 917 253
Enzymes that do cut:
AarI AflIII AleI AlfI ApaI ApoI AvaI BanI
BanII BcgI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI ClaI DrdI EaeI EagI EarI
Eco57MI EcoO109I EcoRV HaeII HindIII MslI MspA1I NaeI
NgoMIV NspI PasI PciI PflMI PspOMI PstI PvuII
SapI SfcI SmaI SmlI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BsgI BsiWI BsmI BspEI BspHI BsrGI BssHII
BstEII BstZ17I Bsu36I BtsI CspCI DraI DraIII EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI ZraI