(Circular) (Six-base) MAPSORT of: pFN21AE6305.seq Check: 1164 from: 1 to: 5935 pFN21AE6305 5935 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 3278 1937 Size: 1161 180 4594 Fragments arranged by size: 4594 1161 180 Acc65I G'GTAC_C Cuts at: 3256 3256 Size: 5935 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 2985 3998 5382 5650 1632 Size: 534 819 1013 1384 268 1917 Fragments arranged by size: 1917 1384 1013 819 534 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 2551 4834 5811 1203 Size: 1348 2283 977 1327 Fragments arranged by size: 2283 1348 1327 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 2966 1166 Size: 1800 4135 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AlwNI CAG_nnn'CTG Cuts at: 2056 2246 2288 2590 3008 3678 5250 2056 Size: 190 42 302 418 670 1572 2741 Fragments arranged by size: 2741 1572 670 418 302 190 42 ApaLI G'TGCA_C Cuts at: 3992 5148 3992 Size: 1156 4779 ApoI r'AATT_y Cuts at: 1493 2363 2390 2426 3042 3244 3470 3622 5731 Size: 870 27 36 616 202 226 152 2109 Cuts at: 5731 1493 Size: 1697 Fragments arranged by size: 2109 1697 870 616 226 202 152 36 27 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2652 3260 1870 Size: 73 709 608 4545 Fragments arranged by size: 4545 709 608 73 AvrII C'CTAG_G Cuts at: 2072 2072 Size: 5935 BamHI G'GATC_C Cuts at: 2904 3265 3632 2904 Size: 361 367 5207 Fragments arranged by size: 5207 367 361 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 3256 4711 618 Size: 325 217 627 322 1147 1455 1842 Fragments arranged by size: 1842 1455 1147 627 325 322 217 BanII G_rGCy'C Cuts at: 1391 3254 1391 Size: 1863 4072 BbeI G_GCGC'C Cuts at: 2113 2113 Size: 5935 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4964 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4126 4160 1903 Size: 34 50 34 2105 34 3678 Fragments arranged by size: 3678 2105 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 2977 1548 Size: 1429 4506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2481 4546 136 Size: 107 122 71 631 605 809 2065 1525 Fragments arranged by size: 2065 1525 809 631 605 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 2199 2531 3352 2199 Size: 332 821 4782 Fragments arranged by size: 4782 821 332 Bme1580I G_kGCm'C Cuts at: 1790 3996 5152 1790 Size: 2206 1156 2573 Fragments arranged by size: 2573 2206 1156 BmgBI CAC'GTC Cuts at: 1318 2552 1318 Size: 1234 4701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3666 4624 442 Size: 416 53 83 399 24 2249 958 1753 Fragments arranged by size: 2249 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2268 4595 1239 Size: 1029 2327 2579 Fragments arranged by size: 2579 2327 1029 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4060 4925 5223 4060 Size: 865 298 4772 Fragments arranged by size: 4772 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4598 882 Size: 617 3099 2219 Fragments arranged by size: 3099 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3726 493 Size: 711 2522 2702 Fragments arranged by size: 2702 2522 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2110 3742 4124 4759 Size: 53 83 186 936 577 1632 382 635 Cuts at: 4759 275 Size: 1451 Fragments arranged by size: 1632 1451 936 635 577 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2982 4368 4766 5040 5187 5598 2982 Size: 1386 398 274 147 411 3319 Fragments arranged by size: 3319 1386 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2724 2754 1178 Size: 30 165 30 1321 30 4359 Fragments arranged by size: 4359 1321 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3138 1186 Size: 1952 3983 BseYI C'CCAG_C Cuts at: 2861 5138 5634 2861 Size: 2277 496 3162 Fragments arranged by size: 3162 2277 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2990 1163 Size: 573 1254 4108 Fragments arranged by size: 4108 1254 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2092 3024 3199 3996 4081 5152 2092 Size: 932 175 797 85 1071 2875 Fragments arranged by size: 2875 1071 932 797 175 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 BsmBI CGTCTCn'nnnn_ Cuts at: 2779 2779 Size: 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 3024 3199 3254 3996 4081 5152 Size: 399 302 932 175 55 742 85 1071 Cuts at: 5152 1391 Size: 2174 Fragments arranged by size: 2174 1071 932 742 399 302 175 85 55 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 2407 3824 5554 2407 Size: 1417 1730 2788 Fragments arranged by size: 2788 1730 1417 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2798 3290 844 Size: 1954 492 3489 Fragments arranged by size: 3489 1954 492 BsrBI CCG'CTC Cuts at: 1394 1823 2062 2203 3822 1394 Size: 429 239 141 1619 3507 Fragments arranged by size: 3507 1619 429 239 141 BsrDI GCAATG_nn' Cuts at: 65 1248 4430 4604 65 Size: 1183 3182 174 1396 Fragments arranged by size: 3182 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4579 4766 1700 Size: 110 2769 187 2869 Fragments arranged by size: 2869 2769 187 110 BsrGI T'GTAC_A Cuts at: 96 2490 96 Size: 2394 3541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5935 BssSI C'ACGA_G Cuts at: 3995 5007 3995 Size: 1012 4923 BstAPI GCAn_nnn'nTGC Cuts at: 794 2014 2056 2755 794 Size: 1220 42 699 3974 Fragments arranged by size: 3974 1220 699 42 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstYI r'GATC_y Cuts at: 1972 2904 3265 3632 4017 4034 5486 5572 5584 Size: 932 361 367 385 17 1452 86 12 Cuts at: 5584 5930 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 932 385 367 361 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3099 3099 Size: 5935 Bsu36I CC'TnA_GG Cuts at: 2939 2939 Size: 5935 BtgI C'CryG_G Cuts at: 513 1065 2536 2964 513 Size: 552 1471 428 3484 Fragments arranged by size: 3484 1471 552 428 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5196 Fragments arranged by size: 5196 683 56 BtsI GCAGTG_nn' Cuts at: 2239 2732 3006 3081 3451 4245 4265 2239 Size: 493 274 75 370 794 20 3909 Fragments arranged by size: 3909 794 493 370 274 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 2395 3239 2395 Size: 844 5091 DraIII CAC_nnn'GTG Cuts at: 2096 2096 Size: 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2728 4942 809 Size: 507 1412 2214 1802 Fragments arranged by size: 2214 1802 1412 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3153 3382 4271 Size: 54 1425 50 275 18 1323 229 889 Cuts at: 4271 5900 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1323 889 275 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3217 3865 1353 Size: 1864 648 3423 Fragments arranged by size: 3423 1864 648 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2013 4522 4896 5042 387 Size: 1064 138 424 2509 374 146 1280 Fragments arranged by size: 2509 1280 1064 424 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2268 2985 3998 4595 5382 5650 Size: 393 534 102 717 1013 597 787 268 Cuts at: 5650 1239 Size: 1524 Fragments arranged by size: 1524 1013 787 717 597 534 393 268 102 EcoO109I rG'GnC_Cy Cuts at: 2034 2914 2034 Size: 880 5055 EcoRI G'AATT_C Cuts at: 2363 2426 3042 3244 3622 2363 Size: 63 616 202 378 4676 Fragments arranged by size: 4676 616 378 202 63 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 2084 2113 5082 1678 Size: 406 29 2969 2531 Fragments arranged by size: 2969 2531 406 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2038 2070 4576 4608 4650 Size: 32 135 32 466 32 2506 32 42 Cuts at: 4650 4682 1373 Size: 32 2626 Fragments arranged by size: 2626 2506 466 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2280 3279 3525 669 Size: 988 281 342 999 246 3079 Fragments arranged by size: 3079 999 988 342 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KasI G'GCGC_C Cuts at: 2109 2109 Size: 5935 KpnI G_GTAC'C Cuts at: 3260 3260 Size: 5935 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5049 5233 1305 Size: 3744 184 2007 Fragments arranged by size: 3744 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4113 Fragments arranged by size: 4113 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2966 3017 3893 4252 4411 518 Size: 648 1800 51 876 359 159 2042 Fragments arranged by size: 2042 1800 876 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 3351 4028 5176 5421 1606 Size: 1745 677 1148 245 2120 Fragments arranged by size: 2120 1745 1148 677 245 NarI GG'CG_CC Cuts at: 2110 2110 Size: 5935 NcoI C'CATG_G Cuts at: 513 1065 2964 513 Size: 552 1899 3484 Fragments arranged by size: 3484 1899 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 3293 4838 1132 Size: 97 2064 1545 2229 Fragments arranged by size: 2229 2064 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 2718 1108 Size: 1610 4325 PfoI T'CCnGG_A Cuts at: 2900 2907 2900 Size: 7 5928 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2914 2914 Size: 5935 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 2874 3505 2874 Size: 631 5304 PstI C_TGCA'G Cuts at: 830 2234 3287 830 Size: 1404 1053 3478 Fragments arranged by size: 3478 1404 1053 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SbfI CC_TGCA'GG Cuts at: 3287 3287 Size: 5935 ScaI AGT'ACT Cuts at: 2453 4183 5895 2453 Size: 1730 1712 2493 Fragments arranged by size: 2493 1730 1712 SfcI C'TryA_G Cuts at: 826 1045 2230 2777 3283 4418 5099 5290 826 Size: 219 1185 547 506 1135 681 191 1471 Fragments arranged by size: 1471 1185 1135 681 547 506 219 191 SfoI GGC'GCC Cuts at: 2111 2111 Size: 5935 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 3262 1872 Size: 1390 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4039 4940 5202 820 Size: 197 926 2096 901 262 1553 Fragments arranged by size: 2096 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 3293 1132 Size: 2161 3774 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 2484 1517 Size: 967 4968 StyI C'CwwG_G Cuts at: 513 1065 2072 2964 3177 3374 513 Size: 552 1007 892 213 197 3074 Fragments arranged by size: 3074 1007 892 552 213 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3962 3979 4132 4317 4762 325 Size: 1255 2382 17 153 185 445 1498 Fragments arranged by size: 2382 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 2490 4181 Size: 274 80 33 51 1443 474 39 1691 Cuts at: 4181 5893 96 Size: 1712 138 Fragments arranged by size: 1712 1691 1443 474 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2724 2756 5895 5927 2724 Size: 32 3139 32 2732 Fragments arranged by size: 3139 2732 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2284 1316 Size: 968 4967 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 3260 1870 Size: 1390 4545 XmnI GAAnn'nnTTC Cuts at: 2998 4064 2998 Size: 1066 4869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI