(Linear) (Six-base) MAPSORT of: KIEE6305.seq Check: 677 from: 1 to: 1230 KIEE6305.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 1093 1230 Size: 1093 137 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 161 980 1230 Size: 161 819 250 Fragments arranged by size: 819 250 161 AflIII A'CryG_T Cuts at: 0 546 1230 Size: 546 684 AleI CACnn'nnGTG Cuts at: 0 961 1230 Size: 961 269 AlwNI CAG_nnn'CTG Cuts at: 0 51 241 283 585 1003 1230 Size: 51 190 42 302 418 227 Fragments arranged by size: 418 302 227 190 51 42 ApoI r'AATT_y Cuts at: 0 358 385 421 1037 1230 Size: 358 27 36 616 193 Fragments arranged by size: 616 358 193 36 27 AvaI C'yCGr_G Cuts at: 0 647 1230 Size: 647 583 AvrII C'CTAG_G Cuts at: 0 67 1230 Size: 67 1163 BamHI G'GATC_C Cuts at: 0 899 1230 Size: 899 331 BanI G'GyrC_C Cuts at: 0 104 1230 Size: 104 1126 BbeI G_GCGC'C Cuts at: 0 108 1230 Size: 108 1122 BclI T'GATC_A Cuts at: 0 972 1230 Size: 972 258 BglI GCCn_nnn'nGGC Cuts at: 0 476 1230 Size: 476 754 BlpI GC'TnA_GC Cuts at: 0 194 526 1230 Size: 194 332 704 Fragments arranged by size: 704 332 194 BmgBI CAC'GTC Cuts at: 0 547 1230 Size: 547 683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 263 1230 Size: 263 967 BsaHI Gr'CG_yC Cuts at: 0 105 1230 Size: 105 1125 BsaWI w'CCGG_w Cuts at: 0 977 1230 Size: 977 253 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 719 749 1230 Size: 719 30 481 Fragments arranged by size: 719 481 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1133 1230 Size: 1133 97 BseYI C'CCAG_C Cuts at: 0 856 1230 Size: 856 374 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 985 1230 Size: 985 245 BsiHKAI G_wGCw'C Cuts at: 0 87 1019 1194 1230 Size: 87 932 175 36 Fragments arranged by size: 932 175 87 36 BsmBI CGTCTCn'nnnn_ Cuts at: 0 774 1230 Size: 774 456 Bsp1286I G_dGCh'C Cuts at: 0 87 1019 1194 1230 Size: 87 932 175 36 Fragments arranged by size: 932 175 87 36 BspHI T'CATG_A Cuts at: 0 402 1230 Size: 402 828 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 793 1230 Size: 793 437 BsrBI CCG'CTC Cuts at: 0 57 198 1230 Size: 57 141 1032 Fragments arranged by size: 1032 141 57 BsrGI T'GTAC_A Cuts at: 0 485 1230 Size: 485 745 BstAPI GCAn_nnn'nTGC Cuts at: 0 9 51 750 1230 Size: 9 42 699 480 Fragments arranged by size: 699 480 42 9 BstYI r'GATC_y Cuts at: 0 899 1230 Size: 899 331 BstZ17I GTA'TAC Cuts at: 0 1094 1230 Size: 1094 136 Bsu36I CC'TnA_GG Cuts at: 0 934 1230 Size: 934 296 BtgI C'CryG_G Cuts at: 0 531 959 1230 Size: 531 428 271 Fragments arranged by size: 531 428 271 BtsI GCAGTG_nn' Cuts at: 0 234 727 1001 1076 1230 Size: 234 493 274 75 154 Fragments arranged by size: 493 274 234 154 75 DraI TTT'AAA Cuts at: 0 390 1230 Size: 390 840 DraIII CAC_nnn'GTG Cuts at: 0 91 1230 Size: 91 1139 DrdI GACnn_nn'nnGTC Cuts at: 0 723 1230 Size: 723 507 EaeI y'GGCC_r Cuts at: 0 1148 1230 Size: 1148 82 EarI CTCTTCn'nnn_ Cuts at: 0 1212 1230 Size: 1212 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 8 1230 Size: 8 1222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 161 263 980 1230 Size: 161 102 717 250 Fragments arranged by size: 717 250 161 102 EcoO109I rG'GnC_Cy Cuts at: 0 29 909 1230 Size: 29 880 321 Fragments arranged by size: 880 321 29 EcoRI G'AATT_C Cuts at: 0 358 421 1037 1230 Size: 358 63 616 193 Fragments arranged by size: 616 358 193 63 HaeII r_GCGC'y Cuts at: 0 79 108 1230 Size: 79 29 1122 Fragments arranged by size: 1122 79 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 33 65 1230 Size: 33 32 1165 Fragments arranged by size: 1165 33 32 HincII GTy'rAC Cuts at: 0 275 1230 Size: 275 955 KasI G'GCGC_C Cuts at: 0 104 1230 Size: 104 1126 MslI CAynn'nnrTG Cuts at: 0 961 1012 1230 Size: 961 51 218 Fragments arranged by size: 961 218 51 NarI GG'CG_CC Cuts at: 0 105 1230 Size: 105 1125 NcoI C'CATG_G Cuts at: 0 959 1230 Size: 959 271 PflMI CCAn_nnn'nTGG Cuts at: 0 713 1230 Size: 713 517 PfoI T'CCnGG_A Cuts at: 0 895 902 1230 Size: 895 7 328 Fragments arranged by size: 895 328 7 PpuMI rG'GwC_Cy Cuts at: 0 909 1230 Size: 909 321 PsiI TTA'TAA Cuts at: 0 869 1230 Size: 869 361 PstI C_TGCA'G Cuts at: 0 229 1230 Size: 229 1001 ScaI AGT'ACT Cuts at: 0 448 1230 Size: 448 782 SfcI C'TryA_G Cuts at: 0 225 772 1230 Size: 225 547 458 Fragments arranged by size: 547 458 225 SfoI GGC'GCC Cuts at: 0 106 1230 Size: 106 1124 StuI AGG'CCT Cuts at: 0 479 1230 Size: 479 751 StyI C'CwwG_G Cuts at: 0 67 959 1172 1230 Size: 67 892 213 58 Fragments arranged by size: 892 213 67 58 TatI w'GTAC_w Cuts at: 0 446 485 1230 Size: 446 39 745 Fragments arranged by size: 745 446 39 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 719 751 1230 Size: 719 32 479 Fragments arranged by size: 719 479 32 Tth111I GACn'n_nGTC Cuts at: 0 279 1230 Size: 279 951 XmnI GAAnn'nnTTC Cuts at: 0 993 1230 Size: 993 237 Enzymes that do cut: AccI AcuI AflIII AleI AlwNI ApoI AvaI AvrII BamHI BanI BbeI BclI BglI BlpI BmgBI BpmI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI MslI NarI NcoI PflMI PfoI PpuMI PsiI PstI ScaI SfcI SfoI StuI StyI TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI BanII BbsI BbvCI BcgI BciVI BfrBI BglII Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsiEI BsiWI BsmI BspEI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI BtgZI ClaI CspCI EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI XcmI XhoI XmaI ZraI