################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BglI BglII BlnI BmyI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HpaI HphI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 472 CGCG CG^CG AcsI 355 AAATTC [GA]^AATT[CT] AfaI 381 GTAC GT^AC AflII 10 CTTAAG C^TTAAG AlwI 192 GGATC GGATC(4/5) AlwNI 549 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 355 AAATTC [GA]^AATT[CT] AvaI 893 CCCGGG C^[CT]CG[GA]G AvaII 987 GGACC G^G[AT]CC BciVI 881 GTATCC GTATCC(6/5) BfaI 506 CTAG C^TAG BfrI 10 CTTAAG C^TTAAG BlpI 943 GCTCAGC GC^T[ACGT]AGC BpmI 634 CTCCAG CTGGAG(16/14) Bpu1102I 943 GCTCAGC GC^T[ACGT]AGC BsaAI 432 TACGTA [CT]AC^GT[GA] BsiYI 649 CCAGAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 649 CCAGAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 129 GTCTC GTCTC(1/5) BsmFI 974 GGGAC GGGAC(10/14) BsoBI 893 CCCGGG C^[CT]CG[GA]G BspMI 622 GCAGGT ACCTGC(4/8) BsrDI 24 CATTGC GCAATG(2/0) BstAPI 28 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 472 CGCG CG^CG BstXI 413 CCAACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 192 GGATCT [GA]^GATC[CT] CelII 943 GCTCAGC GC^T[ACGT]AGC Cfr13I 987 GGACC G^G[ACGT]CC DraII 986 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 469 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 558 CCATGG C^C[GA][CT]GG EarI 43 GAAGAG CTCTTC(1/4) EcoO109I 986 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 558 CCATGG C^C[AT][AT]GG HapII 894 CCGG C^CGG HincII 822 GTCAAC GT[CT]^[GA]AC HindII 822 GTCAAC GT[CT]^[GA]AC HindIII 172 AAGCTT A^AGCTT HpaII 894 CCGG C^CGG Ksp632I 43 GAAGAG CTCTTC(1/4) MaeI 506 CTAG C^TAG MaeII 433 ACGT A^CGT MflI 192 GGATCT [GA]^GATC[CT] MspA1I 552 CAGCTG C[AC]G^C[GT]G MspI 894 CCGG C^CGG MvnI 472 CGCG CG^CG MwoI 28 GCACCAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 558 CCATGG C^CATGG PpuMI 986 AGGACCT [GA]G^G[AT]CC[CT] PvuII 552 CAGCTG CAG^CTG RsaI 381 GTAC GT^AC Sau96I 987 GGACC G^G[ACGT]CC SfaNI 395 GCATC GCATC(5/9) SmaI 893 CCCGGG CCC^GGG SnaBI 432 TACGTA TAC^GTA StyI 558 CCATGG C^C[AT][AT]GG Tsp45I 256 GTGAC ^GT[GC]AC XhoII 192 GGATCT [GA]^GATC[CT] XmaCI 893 CCCGGG C^CCGGG XmaI 893 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BbsI 349 GTCTTC GAAGAC(2/6) 862 GTCTTC BcnI 893 CCCGG CC^[GC]GG 894 CCGGG BpuAI 349 GTCTTC GAAGAC(2/6) 862 GTCTTC BsgI 331 GTGCAG GTGCAG(16/14) 959 GTGCAG BsmI 116 GCATTC GAATGC(1/-1) 539 GCATTC MslI 72 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 399 CATAATTGTG NciI 893 CCCGG CC^[GC]GG 894 CCGGG PstI 196 CTGCAG CTGCA^G 287 CTGCAG TspRI 72 CACTG CA[GC]TG[ACGT][ACGT]^ 419 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BsaJI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII PleI Sau3AI ScrFI SfcI SmlI TfiI Tru9I TseI Tsp509I