(Circular) (Six-base) MAPSORT of: pFN21AE4329.seq Check: 5469 from: 1 to: 5707 pFN21AE4329 5707 bp With 182 enzymes: SgfI * March 28, 2012 13:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2618 2618 Size: 5707 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 3770 5154 5422 1632 Size: 575 1563 1384 268 1917 Fragments arranged by size: 1917 1563 1384 575 268 AflII C'TTAA_G Cuts at: 820 1017 2015 820 Size: 197 998 4512 Fragments arranged by size: 4512 998 197 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 2081 1166 Size: 915 4792 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2559 3450 5022 2559 Size: 891 1572 3244 Fragments arranged by size: 3244 1572 891 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2360 3016 3242 3394 5503 1493 Size: 867 656 226 152 2109 1697 Fragments arranged by size: 2109 1697 867 656 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2898 3032 1870 Size: 73 955 134 4545 Fragments arranged by size: 4545 955 134 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3028 4483 618 Size: 325 217 627 1241 1455 1842 Fragments arranged by size: 1842 1455 1241 627 325 217 BanII G_rGCy'C Cuts at: 1391 3026 1391 Size: 1635 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2347 2860 928 Size: 971 448 513 3775 Fragments arranged by size: 3775 971 513 448 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2897 3599 4809 2897 Size: 702 1210 3795 Fragments arranged by size: 3795 1210 702 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 2949 3124 2949 Size: 175 5532 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2417 2764 3438 Size: 416 53 83 399 24 1000 347 674 Cuts at: 3438 4396 442 Size: 958 1753 Fragments arranged by size: 1753 1000 958 674 416 399 347 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 4367 1239 Size: 1385 1743 2579 Fragments arranged by size: 2579 1743 1385 Bpu10I CC'TnA_GC Cuts at: 2873 2873 Size: 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2578 2939 3832 4697 4995 2578 Size: 361 893 865 298 3290 Fragments arranged by size: 3290 893 865 361 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4370 882 Size: 617 2871 2219 Fragments arranged by size: 2871 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2439 3498 493 Size: 711 1235 1059 2702 Fragments arranged by size: 2702 1235 1059 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 2552 4910 5406 2552 Size: 2358 496 2853 Fragments arranged by size: 2853 2358 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 2985 1163 Size: 573 621 628 3885 Fragments arranged by size: 3885 628 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3768 3853 4924 3768 Size: 85 1071 4551 Fragments arranged by size: 4551 1071 85 BsmI GAATG_Cn' Cuts at: 2121 2544 3218 3311 2121 Size: 423 674 93 4517 Fragments arranged by size: 4517 674 423 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3026 3768 3853 4924 1391 Size: 399 1236 742 85 1071 2174 Fragments arranged by size: 2174 1236 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2618 3062 844 Size: 1774 444 3489 Fragments arranged by size: 3489 1774 444 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2028 4202 4376 65 Size: 1183 780 2174 174 1396 Fragments arranged by size: 2174 1396 1183 780 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 2039 794 Size: 1245 4462 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstXI CCAn_nnnn'nTGG Cuts at: 2425 2425 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2197 3037 3404 3789 3806 5258 5344 5356 Size: 225 840 367 385 17 1452 86 12 Cuts at: 5356 5702 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 840 385 367 346 225 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2563 513 Size: 552 939 559 3657 Fragments arranged by size: 3657 939 559 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 3223 4017 4037 3223 Size: 794 20 4893 Fragments arranged by size: 4893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2479 2479 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672 Size: 54 1425 50 275 18 1324 889 1629 Cuts at: 5672 8 Size: 43 Fragments arranged by size: 1629 1425 1324 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 3637 1353 Size: 690 1594 3423 Fragments arranged by size: 3423 1594 690 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2624 3770 4367 5154 5422 1239 Size: 393 575 417 1146 597 787 268 1524 Fragments arranged by size: 1524 1146 787 597 575 417 393 268 EcoO109I rG'GnC_Cy Cuts at: 2992 2992 Size: 5707 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2195 2195 Size: 5707 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373 Size: 32 135 32 2776 32 42 32 2626 Fragments arranged by size: 2776 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2829 3051 3297 669 Size: 988 281 891 222 246 3079 Fragments arranged by size: 3079 988 891 281 246 222 HindIII A'AGCT_T Cuts at: 748 2177 748 Size: 1429 4278 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2071 2442 4821 5005 1305 Size: 766 371 2379 184 2007 Fragments arranged by size: 2379 2007 766 371 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 2408 3665 4024 4183 518 Size: 648 915 327 1257 359 159 2042 Fragments arranged by size: 2042 1257 915 648 359 327 159 MspA1I CmG'CkG Cuts at: 1606 2559 3123 3800 4948 5193 1606 Size: 953 564 677 1148 245 2120 Fragments arranged by size: 2120 1148 953 677 564 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2563 513 Size: 552 939 559 3657 Fragments arranged by size: 3657 939 559 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PfoI T'CCnGG_A Cuts at: 2640 2810 2640 Size: 170 5537 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2992 2992 Size: 5707 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 2205 2296 3059 830 Size: 1375 91 763 3478 Fragments arranged by size: 3478 1375 763 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2559 2559 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SexAI A'CCwGG_T Cuts at: 3003 3003 Size: 5707 SfcI C'TryA_G Cuts at: 826 1045 2079 2201 2292 3055 4190 4871 5062 Size: 219 1034 122 91 763 1135 681 191 Cuts at: 5062 826 Size: 1471 Fragments arranged by size: 1471 1135 1034 763 681 219 191 122 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 2900 3034 1872 Size: 1028 134 4545 Fragments arranged by size: 4545 1028 134 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 2593 2954 3811 4712 4974 Size: 197 926 72 578 361 857 901 262 Cuts at: 4974 820 Size: 1553 Fragments arranged by size: 1553 926 901 857 578 361 262 197 72 SnaBI TAC'GTA Cuts at: 493 2439 3498 493 Size: 1946 1059 2702 Fragments arranged by size: 2702 1946 1059 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 2563 3146 513 Size: 552 939 559 583 3074 Fragments arranged by size: 3074 939 583 559 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 3734 3751 3904 4089 4534 325 Size: 1255 495 1659 17 153 185 445 1498 Fragments arranged by size: 1659 1498 1255 495 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 3953 5665 Size: 274 80 33 51 1443 408 1568 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1568 1443 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2443 2475 2757 2789 5667 5699 2443 Size: 32 282 32 2878 32 2451 Fragments arranged by size: 2878 2451 282 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 2898 3032 1870 Size: 1028 134 4545 Fragments arranged by size: 4545 1028 134 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI