################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BanI BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsmBI BsmI Bsp1407I BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI Bsu36I Cfr10I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FseI FspI Hin1I HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NotI NsiI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 77 enzymes) # ################################### AccII 1355 CGCG CG^CG AciI 739 CCGC CCGC(-3/-1) AflIII 51 ACATGT A^C[GA][CT]GT AhdI 145 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 610 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 145 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1327 GTGCTC G[AT]GC[AT]^C AspI 673 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 612 GGACC G^G[AT]CC BalI 593 TGGCCA TGG^CCA BamHI 940 GGATCC G^GATCC BbsI 242 GAAGAC GAAGAC(2/6) BbvCI 703 GCTGAGG CCTCAGC(-5/-2) BclI 96 TGATCA T^GATCA BlpI 1023 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1023 GCTTAGC GC^T[ACGT]AGC BpuAI 242 GAAGAC GAAGAC(2/6) BsaAI 1257 CACGTA [CT]AC^GT[GA] BseRI 982 GAGGAG GAGGAG(10/8) BsiHKAI 1327 GTGCTC G[AT]GC[AT]^C BsiWI 1414 CGTACG C^GTACG BsmFI 227 GGGAC GGGAC(10/14) BspHI 99 TCATGA T^CATGA BspLU11I 51 ACATGT A^CATGT BspMI 1196 ACCTGC ACCTGC(4/8) BsrDI 170 GCAATG GCAATG(2/0) Bst1107I 639 GTATAC GTA^TAC BstBI 842 TTCGAA TT^CGAA BstEII 894 GGTTACC G^GT[ACGT]ACC BstPI 894 GGTTACC G^GT[ACGT]ACC BstUI 1355 CGCG CG^CG BstYI 940 GGATCC [GA]^GATC[CT] BstZ17I 639 GTATAC GTA^TAC CelII 1023 GCTTAGC GC^T[ACGT]AGC CfoI 599 GCGC GCG^C DraI 1249 TTTAAA TTT^AAA DraII 611 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 581 CCGTGG C^C[GA][CT]GG EaeI 593 TGGCCA [CT]^GGCC[GA] Eam1105I 145 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1222 GAAGAG CTCTTC(1/4) EcoNI 870 CCTTATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 611 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 894 GGTTACC G^GT[ACGT]ACC FauI 738 CCCGC CCCGC(4/6) FbaI 96 TGATCA T^GATCA HaeII 598 AGCGCC [GA]GCGC^[CT] HhaI 599 GCGC GCG^C HinP1I 599 GCGC G^CGC HpaI 1336 GTTAAC GTT^AAC Ksp632I 1222 GAAGAG CTCTTC(1/4) MflI 940 GGATCC [GA]^GATC[CT] MluNI 593 TGGCCA TGG^CCA MscI 593 TGGCCA TGG^CCA MslI 69 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1355 CGCG CG^CG MwoI 1360 GCTAGCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 1360 GCTAGC G^CTAGC NruI 1354 TCGCGA TCG^CGA NspI 51 ACATGT [GA]CATG^[CT] NspV 842 TTCGAA TT^CGAA PaeR7I 552 CTCGAG C^TCGAG PflFI 673 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 400 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 611 AGGACCT [GA]G^G[AT]CC[CT] PstI 451 CTGCAG CTGCA^G PvuII 454 CAGCTG CAG^CTG RcaI 99 TCATGA T^CATGA SfcI 451 CTGCAG C^T[GA][CT]AG SfuI 842 TTCGAA TT^CGAA SmaI 1370 CCCGGG CCC^GGG Tth111I 673 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 400 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 552 CTCGAG C^TCGAG XhoII 940 GGATCC [GA]^GATC[CT] XmaCI 1370 CCCGGG C^CCGGG XmaI 1370 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 535 GTCTAC GT^[AC][GT]AC 639 GTATAC AvaI 552 CTCGAG C^[CT]CG[GA]G 1370 CCCGGG BanII 622 GGGCTC G[GA]GC[CT]^C 754 GAGCCC BpmI 140 CTGGAG CTGGAG(16/14) 1106 CTGGAG BsgI 547 CTGCAC GTGCAG(16/14) 607 GTGCAG BsoBI 552 CTCGAG C^[CT]CG[GA]G 1370 CCCGGG BspDI 529 ATCGAT AT^CGAT 1186 ATCGAT Cfr13I 612 GGACC G^G[ACGT]CC 786 GGCCC ClaI 529 ATCGAT AT^CGAT 1186 ATCGAT HapII 1371 CCGG C^CGG 1448 CCGG HgaI 245 GACGC GACGC(5/10) 1303 GACGC HincII 106 GTTGAC GT[CT]^[GA]AC 1336 GTTAAC HindII 106 GTTGAC GT[CT]^[GA]AC 1336 GTTAAC HpaII 1371 CCGG C^CGG 1448 CCGG HphI 175 GGTGA GGTGA(8/7) 585 GGTGA MspA1I 454 CAGCTG C[AC]G^C[GT]G 739 CCGCTG MspI 1371 CCGG C^CGG 1448 CCGG NlaIV 657 GGAGCC GG[ACGT]^[ACGT]CC 940 GGATCC Sau96I 612 GGACC G^G[ACGT]CC 786 GGCCC ScaI 778 AGTACT AGT^ACT 800 AGTACT SspI 313 AATATT AAT^ATT 1245 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI NciI NdeII NlaIII PleI RsaI Sau3AI ScrFI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I