Restriction map for
pFN21AE4464
(Circular) (Six-base) MAPSORT of: pFN21AE4464.seq Check: 5788 from: 1 to: 6163
pFN21AE4464 6163 bp
With 182 enzymes: SgfI *
March 28, 2012 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2541 2645 3506 1937
Size: 604 104 861 4594
Fragments arranged by size:
4594 861 604 104
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2764 2942 3246 3412 4226 5610 5878 1632
Size: 1132 178 304 166 814 1384 268 1917
Fragments arranged by size:
1917 1384 1132 814 304 268 178 166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 2056 5062 6039 1203
Size: 853 3006 977 1327
Fragments arranged by size:
3006 1327 977 853
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 2155 4892 2155
Size: 2737 3426
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AlwNI CAG_nnn'CTG
Cuts at: 2620 3906 5478 2620
Size: 1286 1572 3305
Fragments arranged by size:
3305 1572 1286
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 2163 2338 2489 3472 3698 3850 5959 1493
Size: 670 175 151 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 670 226 175 152 151
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2557 3375 3488 1870
Size: 73 614 818 113 4545
Fragments arranged by size:
4545 818 614 113 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3248 3281 3248
Size: 33 6130
BamHI G'GATC_C
Cuts at: 2945 3493 3860 2945
Size: 548 367 5248
Fragments arranged by size:
5248 548 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3484 4939 618
Size: 325 217 627 1697 1455 1842
Fragments arranged by size:
1842 1697 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2631 2763 3482 1391
Size: 1240 132 719 4072
Fragments arranged by size:
4072 1240 719 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2254 928
Size: 971 355 4837
Fragments arranged by size:
4837 971 355
BbvCI CC'TCA_GC
Cuts at: 2709 2709
Size: 6163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4354 4388 1903
Size: 34 2417 34 3678
Fragments arranged by size:
3678 2417 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 2101 1548
Size: 553 5610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4774 136
Size: 107 122 71 631 605 3102 1525
Fragments arranged by size:
3102 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3029 3580 3029
Size: 551 5612
Bme1580I G_kGCm'C
Cuts at: 1790 2453 4224 5380 1790
Size: 663 1771 1156 2573
Fragments arranged by size:
2573 1771 1156 663
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2200 3234 3894
Size: 416 53 83 399 24 783 1034 660
Cuts at: 3894 4852 442
Size: 958 1753
Fragments arranged by size:
1753 1034 958 783 660 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3369 1056
Size: 2313 3850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 3132 4823 1239
Size: 927 966 1691 2579
Fragments arranged by size:
2579 1691 966 927
Bpu10I CC'TnA_GC
Cuts at: 2709 2709
Size: 6163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2209 3320 4288 5153 5451 2209
Size: 1111 968 865 298 2921
Fragments arranged by size:
2921 1111 968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3264 3954 493
Size: 711 2060 690 2702
Fragments arranged by size:
2702 2060 711 690
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3970 4352 4987 275
Size: 53 83 186 936 2437 382 635 1451
Fragments arranged by size:
2437 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 3105 3135 1178
Size: 30 165 30 948 30 724 30 4206
Fragments arranged by size:
4206 948 724 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3002 1186
Size: 1816 4347
BseYI C'CCAG_C
Cuts at: 5366 5862 5366
Size: 496 5667
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 2633 1163
Size: 573 801 96 4693
Fragments arranged by size:
4693 801 573 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3336 4224 4309 5380 3336
Size: 888 85 1071 4119
Fragments arranged by size:
4119 1071 888 85
BsiWI C'GTAC_G
Cuts at: 3419 3419
Size: 6163
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2453 2631 2763 3336 3482 4224 4309
Size: 399 663 178 132 573 146 742 85
Cuts at: 4309 5380 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 663 573 399 178 146
132 85
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 2104 4052 5782 2104
Size: 1948 1730 2485
Fragments arranged by size:
2485 1948 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3210 3451 3518 844
Size: 2366 241 67 3489
Fragments arranged by size:
3489 2366 241 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4050 1394
Size: 429 2227 3507
Fragments arranged by size:
3507 2227 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 4658 4832 65
Size: 1183 934 2476 174 1396
Fragments arranged by size:
2476 1396 1183 934 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4807 4994 1700
Size: 110 2997 187 2869
Fragments arranged by size:
2997 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 4223 5235 4223
Size: 1012 5151
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6163
BstBI TT'CG_AA
Cuts at: 2848 4967 2848
Size: 2119 4044
BstEII G'GTnAC_C
Cuts at: 1184 2899 1184
Size: 1715 4448
BstYI r'GATC_y
Cuts at: 1972 2945 3493 3860 4245 4262 5714 5800 5812
Size: 973 548 367 385 17 1452 86 12
Cuts at: 5812 6158 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 973 548 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2646 2646
Size: 6163
BtgI C'CryG_G
Cuts at: 513 1065 2004 2586 513
Size: 552 939 582 4090
Fragments arranged by size:
4090 939 582 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 2934 3369 3679 4473 4493 2934
Size: 435 310 794 20 4604
Fragments arranged by size:
4604 794 435 310 20
ClaI AT'CG_AT
Cuts at: 1554 2535 3192 1554
Size: 981 657 4525
Fragments arranged by size:
4525 981 657
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2568 2603 529
Size: 35 2004 35 4089
Fragments arranged by size:
4089 2004 35 35
DraI TTT'AAA
Cuts at: 3256 3467 3256
Size: 211 5952
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 3610 4499
Size: 54 1425 50 275 18 768 1012 889
Cuts at: 4499 6128 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1012 889 768 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3222 4093 1353
Size: 1869 871 3423
Fragments arranged by size:
3423 1869 871
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2764 2942 3132 3246 3412 4226
Size: 393 534 598 178 190 114 166 814
Cuts at: 4226 4823 5610 5878 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 814 787 598 597 534 393 268
190 178 166 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2879 2879
Size: 6163
EcoO109I rG'GnC_Cy
Cuts at: 2617 2617
Size: 6163
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2794 2932 2794
Size: 138 6025
FspI TGC'GCA
Cuts at: 4669 4669
Size: 6163
HaeII r_GCGC'y
Cuts at: 1678 2607 5310 1678
Size: 929 2703 2531
Fragments arranged by size:
2703 2531 929
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2141 2148 2173 2207
Size: 32 135 32 544 25 7 25 34
Cuts at: 2207 2239 2351 2383 3434 3466 4804 4836 4878
Size: 32 112 32 1051 32 1338 32 42
Cuts at: 4878 4910 1373
Size: 32 2626
Fragments arranged by size:
2626 1338 1051 544 135 112 42 34
32 32 32 32 32 32 32 25
25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2113 3343 3507 3753 669
Size: 988 281 175 1230 164 246 3079
Fragments arranged by size:
3079 1230 988 281 246 175 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 3343 3753 3343
Size: 410 5753
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2048 3222 5277 5461 1305
Size: 743 1174 2055 184 2007
Fragments arranged by size:
2055 2007 1174 743 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 10
Size: 54 1425 343 768 3573
Fragments arranged by size:
3573 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 4121 4480 4639 518
Size: 648 912 2043 359 159 2042
Fragments arranged by size:
2043 2042 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2461 2746 3579 4256 5404 5649 1606
Size: 855 285 833 677 1148 245 2120
Fragments arranged by size:
2120 1148 855 833 677 285 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4672
Fragments arranged by size:
4672 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 3365 1052
Size: 2313 3850
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NruI TCG'CGA
Cuts at: 3361 3361
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 2060 3521 5066 1132
Size: 97 831 1461 1545 2229
Fragments arranged by size:
2229 1545 1461 831 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6163
PciI A'CATG_T
Cuts at: 2056 5062 2056
Size: 3006 3157
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2411 1108
Size: 1303 4860
PfoI T'CCnGG_A
Cuts at: 3351 3351
Size: 6163
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 5745 5777 4232
Size: 32 1481 32 4618
Fragments arranged by size:
4618 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2617 2617
Size: 6163
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspXI vC'TCGA_Gb
Cuts at: 2557 2557
Size: 6163
PstI C_TGCA'G
Cuts at: 830 2460 3515 830
Size: 1630 1055 3478
Fragments arranged by size:
3478 1630 1055
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 2785 2807 4411 6123 2785
Size: 22 1604 1712 2825
Fragments arranged by size:
2825 1712 1604 22
SfcI C'TryA_G
Cuts at: 826 1045 2456 3511 4646 5327 5518 826
Size: 219 1411 1055 1135 681 191 1471
Fragments arranged by size:
1471 1411 1135 1055 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 3377 3490 1872
Size: 1505 113 4545
Fragments arranged by size:
4545 1505 113
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2224 2557 3335 4267 5168 5430
Size: 197 926 281 333 778 932 901 262
Cuts at: 5430 820
Size: 1553
Fragments arranged by size:
1553 932 926 901 778 333 281 262
197
SnaBI TAC'GTA
Cuts at: 493 3954 493
Size: 3461 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SrfI GCCC'GGGC
Cuts at: 3377 3377
Size: 6163
SspI AAT'ATT
Cuts at: 5 52 2320 3252 4087 5
Size: 47 2268 932 835 2081
Fragments arranged by size:
2268 2081 932 835 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6163
StyI C'CwwG_G
Cuts at: 513 1065 2004 3602 513
Size: 552 939 1598 3074
Fragments arranged by size:
3074 1598 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4190 4207 4360 4545 4990 325
Size: 1255 2610 17 153 185 445 1498
Fragments arranged by size:
2610 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2783 2805 3016
Size: 274 80 33 51 1443 806 22 211
Cuts at: 3016 4409 6121 96
Size: 1393 1712 138
Fragments arranged by size:
1712 1443 1393 806 274 211 138 80
51 33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6123 6155 6123
Size: 32 6131
Tth111I GACn'n_nGTC
Cuts at: 1316 2681 1316
Size: 1365 4798
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6163
XhoI C'TCGA_G
Cuts at: 1943 2557 1943
Size: 614 5549
XmaI C'CCGG_G
Cuts at: 1870 3375 3488 1870
Size: 1505 113 4545
Fragments arranged by size:
4545 1505 113
XmnI GAAnn'nnTTC
Cuts at: 4292 4292
Size: 6163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsmBI BstXI Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI
PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SwaI