(Linear) (Six-base) MAPSORT of: KIEE4464.seq Check: 2796 from: 1 to: 1458 KIEE4464.orf With 182 enzymes: SgfI * March 22, 2012 18:06 .. AccI GT'mk_AC Cuts at: 0 536 640 1458 Size: 536 104 818 Fragments arranged by size: 818 536 104 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 759 937 1241 1407 1458 Size: 759 178 304 166 51 Fragments arranged by size: 759 304 178 166 51 AflIII A'CryG_T Cuts at: 0 51 1458 Size: 51 1407 AhdI GACnn_n'nnGTC Cuts at: 0 150 1458 Size: 150 1308 AlwNI CAG_nnn'CTG Cuts at: 0 615 1458 Size: 615 843 ApoI r'AATT_y Cuts at: 0 158 333 484 1458 Size: 158 175 151 974 Fragments arranged by size: 974 175 158 151 AvaI C'yCGr_G Cuts at: 0 552 1370 1458 Size: 552 818 88 Fragments arranged by size: 818 552 88 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1243 1276 1458 Size: 1243 33 182 Fragments arranged by size: 1243 182 33 BamHI G'GATC_C Cuts at: 0 940 1458 Size: 940 518 BanII G_rGCy'C Cuts at: 0 626 758 1458 Size: 626 132 700 Fragments arranged by size: 700 626 132 BbsI GAAGACnn'nnnn_ Cuts at: 0 249 1458 Size: 249 1209 BbvCI CC'TCA_GC Cuts at: 0 704 1458 Size: 704 754 BclI T'GATC_A Cuts at: 0 96 1458 Size: 96 1362 BlpI GC'TnA_GC Cuts at: 0 1024 1458 Size: 1024 434 Bme1580I G_kGCm'C Cuts at: 0 448 1458 Size: 448 1010 BmrI ACTGGGnnnn_n' Cuts at: 0 195 1229 1458 Size: 195 1034 229 Fragments arranged by size: 1034 229 195 BmtI G_CTAG'C Cuts at: 0 1364 1458 Size: 1364 94 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 161 1127 1458 Size: 161 966 331 Fragments arranged by size: 966 331 161 Bpu10I CC'TnA_GC Cuts at: 0 704 1458 Size: 704 754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 204 1315 1458 Size: 204 1111 143 Fragments arranged by size: 1111 204 143 BsaAI yAC'GTr Cuts at: 0 1259 1458 Size: 1259 199 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 346 376 1100 1130 1458 Size: 346 30 724 30 328 Fragments arranged by size: 724 346 328 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 997 1458 Size: 997 461 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 628 1458 Size: 532 96 830 Fragments arranged by size: 830 532 96 BsiHKAI G_wGCw'C Cuts at: 0 1331 1458 Size: 1331 127 BsiWI C'GTAC_G Cuts at: 0 1414 1458 Size: 1414 44 Bsp1286I G_dGCh'C Cuts at: 0 448 626 758 1331 1458 Size: 448 178 132 573 127 Fragments arranged by size: 573 448 178 132 127 BspHI T'CATG_A Cuts at: 0 99 1458 Size: 99 1359 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1205 1458 Size: 1205 253 BsrDI GCAATG_nn' Cuts at: 0 177 1458 Size: 177 1281 BstBI TT'CG_AA Cuts at: 0 843 1458 Size: 843 615 BstEII G'GTnAC_C Cuts at: 0 894 1458 Size: 894 564 BstYI r'GATC_y Cuts at: 0 940 1458 Size: 940 518 BstZ17I GTA'TAC Cuts at: 0 641 1458 Size: 641 817 BtgI C'CryG_G Cuts at: 0 581 1458 Size: 581 877 BtsI GCAGTG_nn' Cuts at: 0 929 1364 1458 Size: 929 435 94 Fragments arranged by size: 929 435 94 ClaI AT'CG_AT Cuts at: 0 530 1187 1458 Size: 530 657 271 Fragments arranged by size: 657 530 271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 563 598 1458 Size: 563 35 860 Fragments arranged by size: 860 563 35 DraI TTT'AAA Cuts at: 0 1251 1458 Size: 1251 207 EaeI y'GGCC_r Cuts at: 0 593 1458 Size: 593 865 EarI CTCTTCn'nnn_ Cuts at: 0 1217 1458 Size: 1217 241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 161 759 937 1127 1241 1407 1458 Size: 161 598 178 190 114 166 51 Fragments arranged by size: 598 190 178 166 161 114 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 874 1458 Size: 874 584 EcoO109I rG'GnC_Cy Cuts at: 0 612 1458 Size: 612 846 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 789 927 1458 Size: 789 138 531 Fragments arranged by size: 789 531 138 HaeII r_GCGC'y Cuts at: 0 602 1458 Size: 602 856 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 111 136 143 168 202 234 346 378 Size: 111 25 7 25 34 32 112 32 Cuts at: 378 1429 1458 Size: 1051 29 Fragments arranged by size: 1051 112 111 34 32 32 29 25 25 7 HincII GTy'rAC Cuts at: 0 108 1338 1458 Size: 108 1230 120 Fragments arranged by size: 1230 120 108 HpaI GTT'AAC Cuts at: 0 1338 1458 Size: 1338 120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 43 1217 1458 Size: 43 1174 241 Fragments arranged by size: 1174 241 43 MscI TGG'CCA Cuts at: 0 595 1458 Size: 595 863 MslI CAynn'nnrTG Cuts at: 0 73 1458 Size: 73 1385 MspA1I CmG'CkG Cuts at: 0 456 741 1458 Size: 456 285 717 Fragments arranged by size: 717 456 285 NheI G'CTAG_C Cuts at: 0 1360 1458 Size: 1360 98 NruI TCG'CGA Cuts at: 0 1356 1458 Size: 1356 102 NspI r_CATG'y Cuts at: 0 55 1458 Size: 55 1403 PciI A'CATG_T Cuts at: 0 51 1458 Size: 51 1407 PflMI CCAn_nnn'nTGG Cuts at: 0 406 1458 Size: 406 1052 PfoI T'CCnGG_A Cuts at: 0 1346 1458 Size: 1346 112 PpuMI rG'GwC_Cy Cuts at: 0 612 1458 Size: 612 846 PspXI vC'TCGA_Gb Cuts at: 0 552 1458 Size: 552 906 PstI C_TGCA'G Cuts at: 0 455 1458 Size: 455 1003 PvuII CAG'CTG Cuts at: 0 456 1458 Size: 456 1002 ScaI AGT'ACT Cuts at: 0 780 802 1458 Size: 780 22 656 Fragments arranged by size: 780 656 22 SfcI C'TryA_G Cuts at: 0 451 1458 Size: 451 1007 SmaI CCC'GGG Cuts at: 0 1372 1458 Size: 1372 86 SmlI C'TyrA_G Cuts at: 0 219 552 1330 1458 Size: 219 333 778 128 Fragments arranged by size: 778 333 219 128 SrfI GCCC'GGGC Cuts at: 0 1372 1458 Size: 1372 86 SspI AAT'ATT Cuts at: 0 315 1247 1458 Size: 315 932 211 Fragments arranged by size: 932 315 211 TatI w'GTAC_w Cuts at: 0 778 800 1011 1458 Size: 778 22 211 447 Fragments arranged by size: 778 447 211 22 Tth111I GACn'n_nGTC Cuts at: 0 676 1458 Size: 676 782 XhoI C'TCGA_G Cuts at: 0 552 1458 Size: 552 906 XmaI C'CCGG_G Cuts at: 0 1370 1458 Size: 1370 88 Enzymes that do cut: AccI AcuI AflIII AhdI AlwNI ApoI AvaI BaeI BamHI BanII BbsI BbvCI BclI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BsgI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrDI BstBI BstEII BstYI BstZ17I BtgI BtsI ClaI CspCI DraI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NheI NruI NspI PciI PflMI PfoI PpuMI PspXI PstI PvuII ScaI SfcI SmaI SmlI SrfI SspI TatI Tth111I XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BanI BbeI BcgI BciVI BfrBI BglI BglII BmgBI BplI BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI Bsu36I BtgZI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NotI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI StuI StyI SwaI TaqII TstI XbaI XcmI XmnI ZraI