################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HpaI KasI Ksp632I KspI MaeI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 996 GACGTC GACGT^C Acc65I 578 GGTACC G^GTACC AlwNI 391 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 748 CCTGAGG CC^T[ACGT]AGG Asp718I 578 GGTACC G^GTACC BbsI 1111 GTCTTC GAAGAC(2/6) BglI 1028 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 27 GTGCCC G[AGT]GC[ACT]^C BpmI 793 CTGGAG CTGGAG(16/14) BpuAI 1111 GTCTTC GAAGAC(2/6) BsaBI 198 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1286I 27 GTGCCC G[AGT]GC[ACT]^C BsrBI 607 GAGCGG CCGCTC(-3/-3) BsrDI 344 GCAATG GCAATG(2/0) BsrI 864 ACTGG ACTGG(1/-1) BstAPI 390 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 417 CATCC GGATG(2/0) BstXI 557 CCAACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 748 CCTGAGG CC^T[ACGT]AGG DraII 482 GGGTCCT [GA]G^G[ACGT]CC[CT] Eco81I 748 CCTGAGG CC^T[ACGT]AGG EcoO109I 482 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 78 CCAAGG C^C[AT][AT]GG FokI 417 CATCC GGATG(9/13) HincII 235 GTCAAC GT[CT]^[GA]AC HindII 235 GTCAAC GT[CT]^[GA]AC HindIII 263 AAGCTT A^AGCTT KpnI 578 GGTACC GGTAC^C MamI 198 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 401 TTAA T^TAA MslI 223 CACGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 279 CCGCTG C[AC]G^C[GT]G NspI 977 GCATGC [GA]CATG^[CT] PflMI 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 482 GGGTCCT [GA]G^G[AT]CC[CT] ScaI 1010 AGTACT AGT^ACT SexAI 299 ACCTGGT A^CC[AT]GGT SmaI 512 CCCGGG CCC^GGG SmlI 970 CTCAAG C^T[CT][GA]AG SphI 977 GCATGC GCATG^C StuI 839 AGGCCT AGG^CCT StyI 78 CCAAGG C^C[AT][AT]GG Tru9I 401 TTAA T^TAA TspRI 863 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 512 CCCGGG C^CCGGG XmaI 512 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 1025 CGCG CG^CG 1108 CGCG AcyI 922 GACGCC G[GA]^CG[CT]C 996 GACGTC AlwI 204 GATCC GGATC(4/5) 432 GATCC AvaI 512 CCCGGG C^[CT]CG[GA]G 601 CTCGGG BalI 527 TGGCCA TGG^CCA 757 TGGCCA BsaHI 922 GACGCC G[GA]^CG[CT]C 996 GACGTC BsmFI 443 GGGAC GGGAC(10/14) 611 GGGAC BsoBI 512 CCCGGG C^[CT]CG[GA]G 601 CTCGGG BsrFI 121 ACCGGC [GA]^CCGG[CT] 581 ACCGGC BssSI 223 CACGAG CACGAG(-5/-1) 473 CTCGTG BstUI 1025 CGCG CG^CG 1108 CGCG BstYI 203 AGATCC [GA]^GATC[CT] 431 AGATCC Cfr10I 121 ACCGGC [GA]^CCGG[CT] 581 ACCGGC DdeI 371 CTGAG C^T[ACGT]AG 749 CTGAG HgaI 922 GACGC GACGC(5/10) 1109 GCGTC Hin1I 922 GACGCC G[GA]^CG[CT]C 996 GACGTC HinfI 16 GACTC G^A[ACGT]TC 1068 GAGTC MaeII 764 ACGT A^CGT 997 ACGT MaeIII 568 GTGAC ^GT[ACGT]AC 851 GTGAC MflI 203 AGATCC [GA]^GATC[CT] 431 AGATCC MluNI 527 TGGCCA TGG^CCA 757 TGGCCA MscI 527 TGGCCA TGG^CCA 757 TGGCCA MvnI 1025 CGCG CG^CG 1108 CGCG PleI 16 GACTC GAGTC(4/5) 1068 GAGTC Tsp45I 568 GTGAC ^GT[GC]AC 851 GTGAC XhoII 203 AGATCC [GA]^GATC[CT] 431 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BbvI BcnI BsaJI BsiYI BslI BssKI BstNI Cac8I CfoI Cfr13I DpnII EaeI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I