(Circular) (Six-base) MAPSORT of: pFN21AB0443.seq Check: 2890 from: 1 to: 5821 pFN21AB0443 5821 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3005 3631 278 Size: 53 83 186 936 1469 626 2468 Fragments arranged by size: 2468 1469 936 626 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 2583 3142 2583 Size: 559 5262 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2165 2198 2509 2513 2861 3884 5268 5536 Size: 533 33 311 4 348 1023 1384 268 Cuts at: 5536 1632 Size: 1917 Fragments arranged by size: 1917 1384 1023 533 348 311 268 33 4 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2349 1184 Size: 1165 4656 AlwNI CAG_nnn'CTG Cuts at: 2401 3564 5136 2401 Size: 1163 1572 3086 Fragments arranged by size: 3086 1572 1163 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2517 2606 3146 1870 Size: 73 574 89 540 4545 Fragments arranged by size: 4545 574 540 89 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2567 2600 2567 Size: 33 5788 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2115 2583 3022 3142 Size: 325 217 627 300 28 468 439 120 Cuts at: 3142 4597 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 468 439 325 300 217 120 28 BanII G_rGCy'C Cuts at: 1391 3140 1391 Size: 1749 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3109 928 Size: 971 1210 3640 Fragments arranged by size: 3640 1210 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3039 4432 136 Size: 107 122 71 631 605 1367 1393 1525 Fragments arranged by size: 1525 1393 1367 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2036 3882 5038 1790 Size: 246 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 246 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2819 4481 1239 Size: 1580 1662 2579 Fragments arranged by size: 2579 1662 1580 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2960 3946 4811 5109 2960 Size: 986 865 298 3672 Fragments arranged by size: 3672 986 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3612 493 Size: 711 2408 2702 Fragments arranged by size: 2702 2408 711 BsaBI GATnn'nnATC Cuts at: 2207 5815 2207 Size: 3608 2213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2928 3002 3628 4010 Size: 53 83 186 936 1395 74 626 382 Cuts at: 4010 4645 275 Size: 635 1451 Fragments arranged by size: 1451 1395 936 635 626 382 186 83 74 53 BsaWI w'CCGG_w Cuts at: 4254 4652 4926 5073 5484 4254 Size: 398 274 147 411 4591 Fragments arranged by size: 4591 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 2336 2648 2907 3054 5024 5520 2336 Size: 312 259 147 1970 496 2637 Fragments arranged by size: 2637 1970 496 312 259 147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5248 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3882 3967 5038 3882 Size: 85 1071 4665 Fragments arranged by size: 4665 1071 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 3140 3882 3967 5038 1391 Size: 399 246 1104 742 85 1071 2174 Fragments arranged by size: 2174 1104 1071 742 399 246 85 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3176 844 Size: 2332 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2614 3708 1394 Size: 429 791 1094 3507 Fragments arranged by size: 3507 1094 791 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2356 4316 4490 65 Size: 1183 1108 1960 174 1396 Fragments arranged by size: 1960 1396 1183 1108 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2126 2586 4465 4652 1700 Size: 110 316 460 1879 187 2869 Fragments arranged by size: 2869 1879 460 316 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 2228 2478 3881 4893 2228 Size: 250 1403 1012 3156 Fragments arranged by size: 3156 1403 1012 250 BstAPI GCAn_nnn'nTGC Cuts at: 794 2401 794 Size: 1607 4214 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstXI CCAn_nnnn'nTGG Cuts at: 2569 2569 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2208 2436 3151 3518 3903 3920 5372 5458 Size: 236 228 715 367 385 17 1452 86 Cuts at: 5458 5470 5816 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 715 385 367 346 236 228 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2754 2754 Size: 5821 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4330 Fragments arranged by size: 4330 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2937 487 Size: 683 56 1711 3371 Fragments arranged by size: 3371 1711 683 56 BtsI GCAGTG_nn' Cuts at: 3337 4131 4151 3337 Size: 794 20 5007 Fragments arranged by size: 5007 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2128 2532 Size: 54 1425 50 275 18 250 48 404 Cuts at: 2532 2762 3268 4157 5786 8 Size: 230 506 889 1629 43 Fragments arranged by size: 1629 1425 889 506 404 275 250 230 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3751 1353 Size: 2398 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2198 2509 2513 2819 2861 3884 Size: 393 533 33 311 4 306 42 1023 Cuts at: 3884 4481 5268 5536 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1023 787 597 533 393 311 306 268 42 33 4 EcoO109I rG'GnC_Cy Cuts at: 2488 2488 Size: 5821 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 4968 1678 Size: 3290 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3064 3096 4462 4494 4536 Size: 32 135 32 1492 32 1366 32 42 Cuts at: 4536 4568 1373 Size: 32 2626 Fragments arranged by size: 2626 1492 1366 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2242 3165 3411 669 Size: 988 281 304 923 246 3079 Fragments arranged by size: 3079 988 923 304 281 246 HindIII A'AGCT_T Cuts at: 748 2268 748 Size: 1520 4301 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KpnI G_GTAC'C Cuts at: 2587 3146 2587 Size: 559 5262 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 2994 4935 5119 1305 Size: 1674 15 1941 184 2007 Fragments arranged by size: 2007 1941 1674 184 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 2764 10 Size: 54 1425 343 702 230 3067 Fragments arranged by size: 3067 1425 702 343 230 54 MslI CAynn'nnrTG Cuts at: 518 1166 2232 3779 4138 4297 518 Size: 648 1066 1547 359 159 2042 Fragments arranged by size: 2042 1547 1066 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2286 3237 3914 5062 5307 1606 Size: 680 951 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 680 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4330 Fragments arranged by size: 4330 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2986 3179 4724 1132 Size: 97 1757 193 1545 2229 Fragments arranged by size: 2229 1757 1545 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2137 1108 Size: 1029 4792 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2488 Size: 5821 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 3017 4069 5781 3017 Size: 1052 1712 3057 Fragments arranged by size: 3057 1712 1052 SexAI A'CCwGG_T Cuts at: 2304 2304 Size: 5821 SfcI C'TryA_G Cuts at: 826 1045 3169 4304 4985 5176 826 Size: 219 2124 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 2519 3148 1872 Size: 647 629 4545 Fragments arranged by size: 4545 647 629 SmlI C'TyrA_G Cuts at: 820 1017 1943 2975 3925 4826 5088 820 Size: 197 926 1032 950 901 262 1553 Fragments arranged by size: 1553 1032 950 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 2986 3179 1132 Size: 1854 193 3774 Fragments arranged by size: 3774 1854 193 SrfI GCCC'GGGC Cuts at: 2519 2519 Size: 5821 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 2846 1517 Size: 1329 4492 StyI C'CwwG_G Cuts at: 513 1065 2004 2083 3260 513 Size: 552 939 79 1177 3074 Fragments arranged by size: 3074 1177 939 552 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2640 3008 3068 3848 3865 4018 4203 Size: 1255 1060 368 60 780 17 153 185 Cuts at: 4203 4648 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1060 780 445 368 185 153 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3015 4067 5779 Size: 274 80 33 51 1443 1038 1052 1712 Cuts at: 5779 96 Size: 138 Fragments arranged by size: 1712 1443 1052 1038 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2987 3019 5781 5813 2987 Size: 32 2762 32 2995 Fragments arranged by size: 2995 2762 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 2517 3146 1870 Size: 647 629 4545 Fragments arranged by size: 4545 647 629 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3003 3629 276 Size: 53 83 186 936 1469 626 2468 Fragments arranged by size: 2468 1469 936 626 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SwaI XcmI