################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BclI BfrI BglII BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspMI BsrGI Bst1107I BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MflI MluI MspA1I MunI NarI NdeI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 265 GTCTAC GT^[AC][GT]AC AccIII 902 TCCGGA T^CCGGA AflIII 380 ACATGT A^C[GA][CT]GT AlwI 830 GGATC GGATC(4/5) AscI 30 GGCGCGCC GG^CGCGCC AspHI 1050 GTGCTC G[AT]GC[AT]^C BbsI 745 GAAGAC GAAGAC(2/6) BbvCI 678 CCTCAGC CCTCAGC(-5/-2) BcgI 744 CGAAGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1214 GGATAC GTATCC(6/5) BmyI 1050 GTGCTC G[AGT]GC[ACT]^C BpmI 1170 CTCCAG CTGGAG(16/14) BpuAI 745 GAAGAC GAAGAC(2/6) BsaAI 1159 TACGTG [CT]AC^GT[GA] BsaBI 1215 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 902 TCCGGA T^CCGGA BseRI 1024 GAGGAG GAGGAG(10/8) BsgI 345 CTGCAC GTGCAG(16/14) BsiHKAI 1050 GTGCTC G[AT]GC[AT]^C BsmI 1081 GAATGC GAATGC(1/-1) Bsp1286I 1050 GTGCTC G[AGT]GC[ACT]^C BspEI 902 TCCGGA T^CCGGA BspLU11I 380 ACATGT A^CATGT BssHII 31 GCGCGC G^CGCGC BssSI 728 CACGAG CACGAG(-5/-1) BstBI 742 TTCGAA TT^CGAA EcoNI 1233 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 648 ATGCAT ATGCA^T MamI 1215 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 902 TCCGGA T^CCGGA MseI 122 TTAA T^TAA NaeI 17 GCCGGC GCC^GGC NgoMIV 17 GCCGGC G^CCGGC NsiI 648 ATGCAT ATGCA^T NspI 380 ACATGT [GA]CATG^[CT] NspV 742 TTCGAA TT^CGAA PflMI 487 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1018 CTGCAG CTGCA^G SapI 127 GAAGAGC GCTCTTC(1/4) ScaI 938 AGTACT AGT^ACT SfuI 742 TTCGAA TT^CGAA SspI 835 AATATT AAT^ATT TfiI 1185 GATTC G^A[AT]TC Tru9I 122 TTAA T^TAA Tsp45I 300 GTCAC ^GT[GC]AC Van91I 487 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 139 GAATTT [GA]^AATT[CT] 430 AAATTT ApoI 139 GAATTT [GA]^AATT[CT] 430 AAATTT AvaI 170 CCCGAG C^[CT]CG[GA]G 884 CCCGAG BcnI 49 CCCGG CC^[GC]GG 60 CCGGG BglI 44 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 632 GCCCCAAGGGC BsaI 887 GAGACC GGTCTC(1/5) 55 GGTCTC BsaWI 83 ACCGGA [AT]^CCGG[AT] 902 TCCGGA BsmAI 887 GAGAC GTCTC(1/5) 56 GTCTC BsoBI 170 CCCGAG C^[CT]CG[GA]G 884 CCCGAG BsrBI 442 CCGCTC CCGCTC(-3/-3) 9 GAGCGG BsrFI 17 GCCGGC [GA]^CCGG[CT] 76 ACCGGC BstAPI 641 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1155 GCAGTACGTGC Cfr10I 17 GCCGGC [GA]^CCGG[CT] 76 ACCGGC DdeI 527 CTAAG C^T[ACGT]AG 679 CTCAG DrdI 748 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1168 GACTCCAGGGTC EarI 512 CTCTTC CTCTTC(1/4) 127 GAAGAG FauI 287 GCGGG CCCGC(4/6) 1164 GCGGG HphI 586 GGTGA GGTGA(8/7) 881 TCACC Ksp632I 512 CTCTTC CTCTTC(1/4) 127 GAAGAG MaeII 303 ACGT A^CGT 1160 ACGT MaeIII 300 GTCAC ^GT[ACGT]AC 932 GTAAC NciI 49 CCCGG CC^[GC]GG 60 CCGGG PleI 1168 GACTC GAGTC(4/5) 589 GAGTC PpuMI 62 GGGTCCT [GA]G^G[AT]CC[CT] 667 GGGACCC StuI 566 AGGCCT AGG^CCT 1103 AGGCCT TaqI 104 TCGA T^CGA 743 TCGA TthHB8I 104 TCGA T^CGA 743 TCGA XbaI 1188 TCTAGA T^CTAGA 1225 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BalI BbvI BfaI BsaJI BsiYI BslI BsmFI BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MboI MboII MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp509I TspRI