(Circular) (Six-base) MAPSORT of: pFN21AE6313.seq Check: 2375 from: 1 to: 5950 pFN21AE6313 5950 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 3293 1937 Size: 334 1022 4594 Fragments arranged by size: 4594 1022 334 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3161 3227 4013 5397 5665 1632 Size: 1529 66 786 1384 268 1917 Fragments arranged by size: 1917 1529 1384 786 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 2385 4849 5826 1203 Size: 1182 2464 977 1327 Fragments arranged by size: 2464 1327 1182 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 3693 5265 3693 Size: 1572 4378 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 2144 2435 3259 3485 3637 5746 1493 Size: 651 291 824 226 152 2109 1697 Fragments arranged by size: 2109 1697 824 651 291 226 152 AscI GG'CGCG_CC Cuts at: 2036 2036 Size: 5950 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 2175 2889 3275 1870 Size: 73 232 714 386 4545 Fragments arranged by size: 4545 714 386 232 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2307 2340 2307 Size: 33 5917 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3271 4726 618 Size: 325 217 627 1484 1455 1842 Fragments arranged by size: 1842 1484 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3269 1391 Size: 1878 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2757 928 Size: 971 858 4121 Fragments arranged by size: 4121 971 858 BbvCI CC'TCA_GC Cuts at: 2684 2684 Size: 5950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2738 2772 4141 4175 1903 Size: 34 50 34 717 34 1369 34 3678 Fragments arranged by size: 3678 1369 717 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3213 3842 5052 3213 Size: 629 1210 4111 Fragments arranged by size: 4111 1210 629 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BfrBI ATG'CAT Cuts at: 2655 2655 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2055 2643 4561 Size: 107 122 71 631 605 383 588 1918 Cuts at: 4561 136 Size: 1525 Fragments arranged by size: 1918 1525 631 605 588 383 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 4011 5167 1790 Size: 2221 1156 2573 Fragments arranged by size: 2573 2221 1156 BmgBI CAC'GTC Cuts at: 1318 2309 1318 Size: 991 4959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2219 3681 4639 Size: 416 53 83 399 24 802 1462 958 Cuts at: 4639 442 Size: 1753 Fragments arranged by size: 1753 1462 958 802 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2915 2915 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3160 4610 1239 Size: 1921 1450 2579 Fragments arranged by size: 2579 1921 1450 Bpu10I CC'TnA_GC Cuts at: 2684 2684 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 2886 4613 882 Size: 617 567 820 1727 2219 Fragments arranged by size: 2219 1727 820 617 567 BsaAI yAC'GTr Cuts at: 493 1204 3166 3741 493 Size: 711 1962 575 2702 Fragments arranged by size: 2702 1962 711 575 BsaBI GATnn'nnATC Cuts at: 3224 5944 3224 Size: 2720 3230 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3757 4139 4774 275 Size: 53 83 186 936 2224 382 635 1451 Fragments arranged by size: 2224 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2088 2907 4383 4781 5055 5202 5613 2088 Size: 819 1476 398 274 147 411 2425 Fragments arranged by size: 2425 1476 819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5725 Fragments arranged by size: 5725 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3044 1186 Size: 1858 4092 BseYI C'CCAG_C Cuts at: 2250 2724 5153 5649 2250 Size: 474 2429 496 2551 Fragments arranged by size: 2551 2429 496 474 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2335 1163 Size: 573 599 4778 Fragments arranged by size: 4778 599 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3059 4011 4096 5167 3059 Size: 952 85 1071 3842 Fragments arranged by size: 3842 1071 952 85 BsmI GAATG_Cn' Cuts at: 3092 3461 3554 3092 Size: 369 93 5488 Fragments arranged by size: 5488 369 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3059 3269 4011 4096 5167 1391 Size: 399 1269 210 742 85 1071 2174 Fragments arranged by size: 2174 1269 1071 742 399 210 85 BspEI T'CCGG_A Cuts at: 2907 5613 2907 Size: 2706 3244 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2016 2449 3837 1394 Size: 429 193 433 1388 3507 Fragments arranged by size: 3507 1388 433 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 2805 3013 3211 4445 4619 65 Size: 1183 1557 208 198 1234 174 1396 Fragments arranged by size: 1557 1396 1234 1183 208 198 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2022 2081 4594 4781 1700 Size: 110 212 59 2513 187 2869 Fragments arranged by size: 2869 2513 212 187 110 59 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 2036 1925 Size: 111 5839 BssSI C'ACGA_G Cuts at: 2733 4010 5022 2733 Size: 1277 1012 3661 Fragments arranged by size: 3661 1277 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2652 3166 794 Size: 1858 514 3578 Fragments arranged by size: 3578 1858 514 BstBI TT'CG_AA Cuts at: 2748 4754 2748 Size: 2006 3944 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 2457 3060 513 Size: 552 1053 339 603 3403 Fragments arranged by size: 3403 1053 603 552 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 3021 3051 3466 4260 4280 3021 Size: 30 415 794 20 4691 Fragments arranged by size: 4691 794 415 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2964 2999 529 Size: 35 2400 35 3480 Fragments arranged by size: 3480 2400 35 35 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2759 3179 4957 809 Size: 507 1443 420 1778 1802 Fragments arranged by size: 1802 1778 1443 507 420 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2769 2931 Size: 54 1425 50 275 18 253 686 162 Cuts at: 2931 2985 3063 3397 4286 5915 8 Size: 54 78 334 889 1629 43 Fragments arranged by size: 1629 1425 889 686 334 275 253 162 78 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2523 3880 1353 Size: 774 396 1357 3423 Fragments arranged by size: 3423 1357 774 396 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3160 3161 3227 4013 4610 5397 5665 Size: 393 1528 1 66 786 597 787 268 Cuts at: 5665 1239 Size: 1524 Fragments arranged by size: 1528 1524 787 786 597 393 268 66 1 EcoNI CCTnn'n_nnAGG Cuts at: 3242 3242 Size: 5950 EcoO109I rG'GnC_Cy Cuts at: 2068 2636 2673 2068 Size: 568 37 5345 Fragments arranged by size: 5345 568 37 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 5097 1678 Size: 3419 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 2727 2759 2820 Size: 32 135 32 926 32 197 32 61 Cuts at: 2820 2852 4591 4623 4665 4697 1373 Size: 32 1739 32 42 32 2626 Fragments arranged by size: 2626 1739 926 197 135 61 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5064 5248 1305 Size: 3759 184 2007 Fragments arranged by size: 3759 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2771 2933 3065 10 Size: 54 1425 343 939 162 132 2895 Fragments arranged by size: 2895 1425 939 343 162 132 54 MslI CAynn'nnrTG Cuts at: 518 1166 2606 2810 2901 3908 4267 4426 518 Size: 648 1440 204 91 1007 359 159 2042 Fragments arranged by size: 2042 1440 1007 648 359 204 159 91 MspA1I CmG'CkG Cuts at: 1606 3366 4043 5191 5436 1606 Size: 1760 677 1148 245 2120 Fragments arranged by size: 2120 1760 1148 677 245 NaeI GCC'GGC Cuts at: 2024 2024 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 2118 2457 3060 513 Size: 552 1053 339 603 3403 Fragments arranged by size: 3403 1053 603 552 339 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NgoMIV G'CCGG_C Cuts at: 2022 2022 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NsiI A_TGCA'T Cuts at: 2657 2657 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2389 3308 4853 1132 Size: 97 1160 919 1545 2229 Fragments arranged by size: 2229 1545 1160 919 97 PasI CC'CwG_GG Cuts at: 1348 2857 1348 Size: 1509 4441 PciI A'CATG_T Cuts at: 2385 4849 2385 Size: 2464 3486 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2498 1108 Size: 902 488 4560 Fragments arranged by size: 4560 902 488 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2673 2068 Size: 605 5345 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PstI C_TGCA'G Cuts at: 830 3027 3302 830 Size: 2197 275 3478 Fragments arranged by size: 3478 2197 275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SanDI GG'GwC_CC Cuts at: 2673 2673 Size: 5950 SapI GCTCTTCn'nnn_ Cuts at: 2127 2127 Size: 5950 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 2945 4198 5910 2945 Size: 1253 1712 2985 Fragments arranged by size: 2985 1712 1253 SfcI C'TryA_G Cuts at: 826 1045 2343 3023 3078 3298 4433 5114 5305 Size: 219 1298 680 55 220 1135 681 191 Cuts at: 5305 826 Size: 1471 Fragments arranged by size: 1471 1298 1135 681 680 220 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 2842 3874 5 Size: 47 2790 1032 2081 Fragments arranged by size: 2790 2081 1032 47 StuI AGG'CCT Cuts at: 1517 2573 3110 1517 Size: 1056 537 4357 Fragments arranged by size: 4357 1056 537 StyI C'CwwG_G Cuts at: 513 1065 2118 2337 2457 2568 2640 3060 3389 Size: 552 1053 219 120 111 72 420 329 Cuts at: 3389 513 Size: 3074 Fragments arranged by size: 3074 1053 552 420 329 219 120 111 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3977 3994 4147 4332 4777 325 Size: 1255 2397 17 153 185 445 1498 Fragments arranged by size: 2397 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2943 4196 5908 Size: 274 80 33 51 1443 966 1253 1712 Cuts at: 5908 96 Size: 138 Fragments arranged by size: 1712 1443 1253 966 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3193 3230 3286 3193 Size: 37 56 5857 Fragments arranged by size: 5857 56 37 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI