(Linear) (Six-base) MAPSORT of: KIEE6313.seq Check: 2946 from: 1 to: 1245 KIEE6313.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 266 1245 Size: 266 979 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1156 1222 1245 Size: 1156 66 23 Fragments arranged by size: 1156 66 23 AflIII A'CryG_T Cuts at: 0 380 1245 Size: 380 865 ApoI r'AATT_y Cuts at: 0 139 430 1245 Size: 139 291 815 Fragments arranged by size: 815 291 139 AscI GG'CGCG_CC Cuts at: 0 31 1245 Size: 31 1214 AvaI C'yCGr_G Cuts at: 0 170 884 1245 Size: 170 714 361 Fragments arranged by size: 714 361 170 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 302 335 1245 Size: 302 33 910 Fragments arranged by size: 910 302 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 752 1245 Size: 752 493 BbvCI CC'TCA_GC Cuts at: 0 679 1245 Size: 679 566 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 733 767 1245 Size: 733 34 478 Fragments arranged by size: 733 478 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1208 1245 Size: 1208 37 BfrBI ATG'CAT Cuts at: 0 650 1245 Size: 650 595 BglI GCCn_nnn'nGGC Cuts at: 0 50 638 1245 Size: 50 588 607 Fragments arranged by size: 607 588 50 BmgBI CAC'GTC Cuts at: 0 304 1245 Size: 304 941 BmrI ACTGGGnnnn_n' Cuts at: 0 214 1245 Size: 214 1031 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 910 1245 Size: 910 335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1155 1245 Size: 1155 90 Bpu10I CC'TnA_GC Cuts at: 0 679 1245 Size: 679 566 BsaI GGTCTCn'nnnn_ Cuts at: 0 61 881 1245 Size: 61 820 364 Fragments arranged by size: 820 364 61 BsaAI yAC'GTr Cuts at: 0 1161 1245 Size: 1161 84 BsaBI GATnn'nnATC Cuts at: 0 1219 1245 Size: 1219 26 BsaWI w'CCGG_w Cuts at: 0 83 902 1245 Size: 83 819 343 Fragments arranged by size: 819 343 83 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1039 1245 Size: 1039 206 BseYI C'CCAG_C Cuts at: 0 245 719 1245 Size: 245 474 526 Fragments arranged by size: 526 474 245 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 330 1245 Size: 330 915 BsiHKAI G_wGCw'C Cuts at: 0 1054 1245 Size: 1054 191 BsmI GAATG_Cn' Cuts at: 0 1087 1245 Size: 1087 158 Bsp1286I G_dGCh'C Cuts at: 0 1054 1245 Size: 1054 191 BspEI T'CCGG_A Cuts at: 0 902 1245 Size: 902 343 BsrBI CCG'CTC Cuts at: 0 11 444 1245 Size: 11 433 801 Fragments arranged by size: 801 433 11 BsrDI GCAATG_nn' Cuts at: 0 800 1008 1206 1245 Size: 800 208 198 39 Fragments arranged by size: 800 208 198 39 BsrFI r'CCGG_y Cuts at: 0 17 76 1245 Size: 17 59 1169 Fragments arranged by size: 1169 59 17 BssHII G'CGCG_C Cuts at: 0 31 1245 Size: 31 1214 BssSI C'ACGA_G Cuts at: 0 728 1245 Size: 728 517 BstAPI GCAn_nnn'nTGC Cuts at: 0 647 1161 1245 Size: 647 514 84 Fragments arranged by size: 647 514 84 BstBI TT'CG_AA Cuts at: 0 743 1245 Size: 743 502 BtgI C'CryG_G Cuts at: 0 113 452 1055 1245 Size: 113 339 603 190 Fragments arranged by size: 603 339 190 113 BtsI GCAGTG_nn' Cuts at: 0 1016 1046 1245 Size: 1016 30 199 Fragments arranged by size: 1016 199 30 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 959 994 1245 Size: 959 35 251 Fragments arranged by size: 959 251 35 DrdI GACnn_nn'nnGTC Cuts at: 0 754 1174 1245 Size: 754 420 71 Fragments arranged by size: 754 420 71 EaeI y'GGCC_r Cuts at: 0 78 764 926 980 1058 1245 Size: 78 686 162 54 78 187 Fragments arranged by size: 686 187 162 78 78 54 EarI CTCTTCn'nnn_ Cuts at: 0 122 518 1245 Size: 122 396 727 Fragments arranged by size: 727 396 122 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1155 1156 1222 1245 Size: 1155 1 66 23 Fragments arranged by size: 1155 66 23 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 1237 1245 Size: 1237 8 EcoO109I rG'GnC_Cy Cuts at: 0 63 631 668 1245 Size: 63 568 37 577 Fragments arranged by size: 577 568 63 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 493 525 722 754 815 847 1245 Size: 493 32 197 32 61 32 398 Fragments arranged by size: 493 398 197 61 32 32 32 MscI TGG'CCA Cuts at: 0 766 928 1060 1245 Size: 766 162 132 185 Fragments arranged by size: 766 185 162 132 MslI CAynn'nnrTG Cuts at: 0 601 805 896 1245 Size: 601 204 91 349 Fragments arranged by size: 601 349 204 91 NaeI GCC'GGC Cuts at: 0 19 1245 Size: 19 1226 NcoI C'CATG_G Cuts at: 0 113 452 1055 1245 Size: 113 339 603 190 Fragments arranged by size: 603 339 190 113 NgoMIV G'CCGG_C Cuts at: 0 17 1245 Size: 17 1228 NsiI A_TGCA'T Cuts at: 0 652 1245 Size: 652 593 NspI r_CATG'y Cuts at: 0 384 1245 Size: 384 861 PasI CC'CwG_GG Cuts at: 0 852 1245 Size: 852 393 PciI A'CATG_T Cuts at: 0 380 1245 Size: 380 865 PflMI CCAn_nnn'nTGG Cuts at: 0 493 1245 Size: 493 752 PpuMI rG'GwC_Cy Cuts at: 0 63 668 1245 Size: 63 605 577 Fragments arranged by size: 605 577 63 PstI C_TGCA'G Cuts at: 0 1022 1245 Size: 1022 223 SanDI GG'GwC_CC Cuts at: 0 668 1245 Size: 668 577 SapI GCTCTTCn'nnn_ Cuts at: 0 122 1245 Size: 122 1123 ScaI AGT'ACT Cuts at: 0 940 1245 Size: 940 305 SfcI C'TryA_G Cuts at: 0 338 1018 1073 1245 Size: 338 680 55 172 Fragments arranged by size: 680 338 172 55 SspI AAT'ATT Cuts at: 0 837 1245 Size: 837 408 StuI AGG'CCT Cuts at: 0 568 1105 1245 Size: 568 537 140 Fragments arranged by size: 568 537 140 StyI C'CwwG_G Cuts at: 0 113 332 452 563 635 1055 1245 Size: 113 219 120 111 72 420 190 Fragments arranged by size: 420 219 190 120 113 111 72 TatI w'GTAC_w Cuts at: 0 938 1245 Size: 938 307 XbaI T'CTAG_A Cuts at: 0 1188 1225 1245 Size: 1188 37 20 Fragments arranged by size: 1188 37 20 Enzymes that do cut: AccI AcuI AflIII ApoI AscI AvaI BaeI BbsI BbvCI BcgI BciVI BfrBI BglI BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BtgI BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MscI MslI NaeI NcoI NgoMIV NsiI NspI PasI PciI PflMI PpuMI PstI SanDI SapI ScaI SfcI SspI StuI StyI TatI XbaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AseI AsiSI AvrII BamHI BanI BanII BbeI BclI BglII BlpI Bme1580I BmtI BpuEI BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BspMI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NarI NdeI NheI NotI NruI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TaqII TstI Tth111I XcmI XhoI XmaI XmnI ZraI