################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 730 AAATTT [GA]^AATT[CT] AlwNI 295 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 36 AGCGCT AGC^GCT ApoI 730 AAATTT [GA]^AATT[CT] AspHI 244 GAGCAC G[AT]GC[AT]^C AvaII 870 GGTCC G^G[AT]CC BalI 326 TGGCCA TGG^CCA BanII 138 GGGCTC G[GA]GC[CT]^C BbvCI 393 GCTGAGG CCTCAGC(-5/-2) BsaI 42 GGTCTC GGTCTC(1/5) BseRI 280 GAGGAG GAGGAG(10/8) BsiEI 86 CGGCCG CG[GA][CT]^CG BsiHKAI 244 GAGCAC G[AT]GC[AT]^C BspHI 662 TCATGA T^CATGA BsrBI 12 GAGCGG CCGCTC(-3/-3) BssSI 569 CACGAG CACGAG(-5/-1) BstAPI 765 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1132 GGTCACC G^GT[ACGT]ACC BstF5I 675 GGATG GGATG(2/0) BstPI 1132 GGTCACC G^GT[ACGT]ACC DraII 177 GGGGCCT [GA]G^G[ACGT]CC[CT] EagI 86 CGGCCG C^GGCCG EclXI 86 CGGCCG C^GGCCG Eco47III 36 AGCGCT AGC^GCT Eco52I 86 CGGCCG C^GGCCG EcoNI 252 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 177 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1132 GGTCACC G^GT[ACGT]ACC FauI 627 GCGGG CCCGC(4/6) FokI 675 GGATG GGATG(9/13) HaeII 36 AGCGCT [GA]GCGC^[CT] KspI 89 CCGCGG CCGC^GG MluNI 326 TGGCCA TGG^CCA MscI 326 TGGCCA TGG^CCA MseI 1007 TTAA T^TAA NheI 372 GCTAGC G^CTAGC PaeR7I 1079 CTCGAG C^TCGAG PflMI 936 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 766 CAGCTG CAG^CTG RcaI 662 TCATGA T^CATGA SacII 89 CCGCGG CCGC^GG SexAI 476 ACCTGGT A^CC[AT]GGT SphI 761 GCATGC GCATG^C Tru9I 1007 TTAA T^TAA Tsp509I 731 AATT ^AATT Van91I 936 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 471 TCTAGA T^CTAGA XhoI 1079 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 90 CGCG CG^CG 107 CGCG AvaI 32 CCCGAG C^[CT]CG[GA]G 1079 CTCGAG BanI 916 GGTGCC G^G[CT][GA]CC 923 GGCACC BfaI 373 CTAG C^TAG 472 CTAG BpmI 1023 CTCCAG CTGGAG(16/14) 289 CTGGAG BsmBI 167 GAGACG CGTCTC(1/5) 910 GAGACG BsmFI 226 GTCCC GGGAC(10/14) 871 GTCCC BsoBI 32 CCCGAG C^[CT]CG[GA]G 1079 CTCGAG BspMI 1136 ACCTGC ACCTGC(4/8) 294 GCAGGT BsrI 229 CCAGT ACTGG(1/-1) 786 CCAGT BstUI 90 CGCG CG^CG 107 CGCG CfoI 37 GCGC GCG^C 94 GCGC Cfr13I 178 GGGCC G^G[ACGT]CC 870 GGTCC DsaI 89 CCGCGG C^C[GA][CT]GG 965 CCACGG HhaI 37 GCGC GCG^C 94 GCGC HinP1I 37 GCGC G^CGC 94 GCGC MaeI 373 CTAG C^TAG 472 CTAG MaeII 125 ACGT A^CGT 422 ACGT MboII 558 GAAGA GAAGA(8/7) 1047 GAAGA MvnI 90 CGCG CG^CG 107 CGCG NspI 761 GCATGC [GA]CATG^[CT] 992 ACATGC PstI 688 CTGCAG CTGCA^G 1138 CTGCAG Sau96I 178 GGGCC G^G[ACGT]CC 870 GGTCC SmlI 1012 CTTGAG C^T[CT][GA]AG 1079 CTCGAG TaqI 489 TCGA T^CGA 1080 TCGA TfiI 342 GATTC G^A[AT]TC 777 GAATC TthHB8I 489 TCGA T^CGA 1080 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI BbvI BclI BcnI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I DdeI DpnII EaeI EcoRII FbaI Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SfaNI SfcI TseI Tsp45I TspRI