(Circular) (Six-base) MAPSORT of: pFN21AE6337.seq Check: 8437 from: 1 to: 5860 pFN21AE6337 5860 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2290 3150 2290 Size: 860 5000 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3923 5307 5575 1632 Size: 2291 1384 268 1917 Fragments arranged by size: 2291 1917 1384 268 AfeI AGC'GCT Cuts at: 2043 2043 Size: 5860 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 2259 1166 Size: 1093 4767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2952 1184 Size: 1768 4092 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2672 2704 2672 Size: 32 5828 AlwNI CAG_nnn'CTG Cuts at: 2305 3603 5175 2305 Size: 1298 1572 2990 Fragments arranged by size: 2990 1572 1298 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 2735 3169 3395 3547 5656 1493 Size: 1242 434 226 152 2109 1697 Fragments arranged by size: 2109 1697 1242 434 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2037 3084 3185 1870 Size: 73 94 1047 101 4545 Fragments arranged by size: 4545 1047 101 94 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2921 2928 3181 4636 618 Size: 325 217 627 1134 7 253 1455 1842 Fragments arranged by size: 1842 1455 1134 627 325 253 217 7 BanII G_rGCy'C Cuts at: 1391 2147 3179 1391 Size: 756 1032 4072 Fragments arranged by size: 4072 1032 756 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4889 BbvCI CC'TCA_GC Cuts at: 2399 2399 Size: 5860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 2487 2748 3060 1548 Size: 939 261 312 4348 Fragments arranged by size: 4348 939 312 261 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4471 136 Size: 107 122 71 631 605 337 2462 1525 Fragments arranged by size: 2462 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2926 3921 5077 1790 Size: 1136 995 1156 2573 Fragments arranged by size: 2573 1156 1136 995 BmgBI CAC'GTC Cuts at: 1318 2131 1318 Size: 813 5047 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2228 3591 4549 Size: 416 53 83 399 24 811 1363 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1363 958 811 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2381 1056 Size: 1325 4535 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2398 2536 3043 2398 Size: 138 507 5215 Fragments arranged by size: 5215 507 138 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 3013 4520 1239 Size: 1076 698 1507 2579 Fragments arranged by size: 2579 1507 1076 698 Bpu10I CC'TnA_GC Cuts at: 2187 2399 2187 Size: 212 5648 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3038 3985 4850 5148 3038 Size: 947 865 298 3750 Fragments arranged by size: 3750 947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2053 4523 882 Size: 617 554 2470 2219 Fragments arranged by size: 2470 2219 617 554 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3667 4049 4684 275 Size: 53 83 186 936 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2163 2193 2856 2886 1178 Size: 30 165 30 760 30 663 30 4152 Fragments arranged by size: 4152 760 663 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 1186 Size: 1114 4746 BseYI C'CCAG_C Cuts at: 5063 5559 5063 Size: 496 5364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2288 2798 2916 1163 Size: 573 552 510 118 4107 Fragments arranged by size: 4107 573 552 510 118 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 3310 4071 5099 1540 Size: 275 186 93 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 93 BsiHKAI G_wGCw'C Cuts at: 2253 3921 4006 5077 2253 Size: 1668 85 1071 3036 Fragments arranged by size: 3036 1668 1071 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 BsmBI CGTCTCn'nnnn_ Cuts at: 2166 2909 2166 Size: 743 5117 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2253 2926 3179 3921 4006 5077 Size: 399 357 106 673 253 742 85 1071 Cuts at: 5077 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 673 399 357 253 106 85 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 2667 3749 5479 2667 Size: 1082 1730 3048 Fragments arranged by size: 3048 1730 1082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2290 3150 3215 844 Size: 1446 860 65 3489 Fragments arranged by size: 3489 1446 860 65 BsrBI CCG'CTC Cuts at: 1394 1823 2019 3747 1394 Size: 429 196 1728 3507 Fragments arranged by size: 3507 1728 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 2574 3920 4932 2574 Size: 1346 1012 3502 Fragments arranged by size: 3502 1346 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2776 794 Size: 1982 3878 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 3137 1184 Size: 1953 3907 BstYI r'GATC_y Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855 Size: 1218 367 385 17 1452 86 12 346 Cuts at: 5855 1972 Size: 1977 Fragments arranged by size: 1977 1452 1218 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2970 513 Size: 552 939 90 876 3403 Fragments arranged by size: 3403 939 876 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 4253 Fragments arranged by size: 4253 868 683 56 BtsI GCAGTG_nn' Cuts at: 2643 2702 2952 3376 4170 4190 2643 Size: 59 250 424 794 20 4313 Fragments arranged by size: 4313 794 424 250 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2331 2973 Size: 54 1425 50 275 18 261 240 642 Cuts at: 2973 3307 4196 5825 8 Size: 334 889 1629 43 Fragments arranged by size: 1629 1425 889 642 334 275 261 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2091 3307 1537 Size: 275 279 1216 4090 Fragments arranged by size: 4090 1216 279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3790 1353 Size: 2437 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2041 4447 4821 4967 387 Size: 1064 138 452 2406 374 146 1280 Fragments arranged by size: 2406 1280 1064 452 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 3013 3923 4520 5307 5575 1239 Size: 393 683 698 910 597 787 268 1524 Fragments arranged by size: 1524 910 787 698 683 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2261 2261 Size: 5860 EcoO109I rG'GnC_Cy Cuts at: 2183 2183 Size: 5860 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2431 2623 2431 Size: 192 5668 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2045 5007 1678 Size: 367 2962 2531 Fragments arranged by size: 2962 2531 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 2800 2832 4501 Size: 32 135 32 459 32 737 32 1669 Cuts at: 4501 4533 4575 4607 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1669 737 459 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2391 3018 4974 5158 1305 Size: 1086 627 1956 184 2007 Fragments arranged by size: 2007 1956 1086 627 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 10 Size: 54 1425 343 501 3537 Fragments arranged by size: 3537 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 2535 2975 3818 4177 4336 518 Size: 648 1093 276 440 843 359 159 2042 Fragments arranged by size: 2042 1093 843 648 440 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2028 2096 2228 2590 2773 3276 3953 5101 Size: 422 68 132 362 183 503 677 1148 Cuts at: 5101 5346 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 503 422 362 245 183 132 68 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4369 Fragments arranged by size: 4369 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 2377 1052 Size: 1325 4535 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2770 3001 3218 4763 1132 Size: 97 1541 231 217 1545 2229 Fragments arranged by size: 2229 1545 1541 231 217 97 PasI CC'CwG_GG Cuts at: 1348 2063 1348 Size: 715 5145 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2947 1108 Size: 1839 4021 PfoI T'CCnGG_A Cuts at: 2167 2244 2167 Size: 77 5783 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PstI C_TGCA'G Cuts at: 830 2697 3147 3212 830 Size: 1867 450 65 3478 Fragments arranged by size: 3478 1867 450 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2773 2773 Size: 5860 SacII CC_GC'GG Cuts at: 2097 2097 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SexAI A'CCwGG_T Cuts at: 2481 2481 Size: 5860 SfcI C'TryA_G Cuts at: 826 1045 2693 2890 3100 3143 3208 4343 5024 Size: 219 1648 197 210 43 65 1135 681 Cuts at: 5024 5215 826 Size: 191 1471 Fragments arranged by size: 1648 1471 1135 681 219 210 197 191 65 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3017 3084 3964 4865 5127 820 Size: 197 926 1074 67 880 901 262 1553 Fragments arranged by size: 1553 1074 926 901 880 262 197 67 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 2770 3218 1132 Size: 1638 448 3774 Fragments arranged by size: 3774 1638 448 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 3299 513 Size: 552 939 1295 3074 Fragments arranged by size: 3074 1295 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2946 3125 3887 3904 4057 4242 4687 Size: 1255 1366 179 762 17 153 185 445 Cuts at: 4687 325 Size: 1498 Fragments arranged by size: 1498 1366 1255 762 445 185 179 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 4106 5818 Size: 274 80 33 51 1443 537 1592 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1712 1592 1443 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 2856 2888 5820 5852 2163 Size: 32 661 32 2932 32 2171 Fragments arranged by size: 2932 2171 661 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 2476 3196 2476 Size: 720 5140 XhoI C'TCGA_G Cuts at: 1943 3084 1943 Size: 1141 4719 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI