################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XcmI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcsI 746 GAATTT [GA]^AATT[CT] AcyI 1193 GGCGTC G[GA]^CG[CT]C AflIII 564 ACGTGT A^C[GA][CT]GT Alw44I 982 GTGCAC G^TGCAC AlwNI 638 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 148 AGCGCT AGC^GCT ApaLI 982 GTGCAC G^TGCAC ApoI 746 GAATTT [GA]^AATT[CT] Asp700I 765 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 982 GTGCAC G[AT]GC[AT]^C AspI 515 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BlpI 1002 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1002 GCTAAGC GC^T[ACGT]AGC BsaHI 1193 GGCGTC G[GA]^CG[CT]C BsaWI 99 ACCGGA [AT]^CCGG[AT] BsiEI 97 CGACCG CG[GA][CT]^CG BsiHKAI 982 GTGCAC G[AT]GC[AT]^C BspDI 1159 ATCGAT AT^CGAT BsrBI 65 GAGCGG CCGCTC(-3/-3) BssSI 740 CTCGTG CACGAG(-5/-1) BstAPI 208 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 598 GGTGACC G^GT[ACGT]ACC BstPI 598 GGTGACC G^GT[ACGT]ACC CelII 1002 GCTAAGC GC^T[ACGT]AGC CfoI 149 GCGC GCG^C ClaI 1159 ATCGAT AT^CGAT DraIII 581 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 148 AGCGCT AGC^GCT EcoNI 867 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 598 GGTGACC G^GT[ACGT]ACC EcoRV 1213 GATATC GAT^ATC EcoT14I 815 CCATGG C^C[AT][AT]GG HaeII 148 AGCGCT [GA]GCGC^[CT] HhaI 149 GCGC GCG^C Hin1I 1193 GGCGTC G[GA]^CG[CT]C HinP1I 149 GCGC G^CGC MslI 754 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 80 CCGCTG C[AC]G^C[GT]G NcoI 815 CCATGG C^CATGG PflFI 515 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 242 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 707 CTGCAG CTGCA^G SapI 765 GAAGAGC GCTCTTC(1/4) SmaI 36 CCCGGG CCC^GGG StuI 864 AGGCCT AGG^CCT StyI 815 CCATGG C^C[AT][AT]GG TfiI 854 GATTC G^A[AT]TC Tth111I 515 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 242 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 36 CCCGGG C^CCGGG XmaI 36 CCCGGG C^CCGGG XmnI 765 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaII 7 GGTCC G^G[AT]CC 1116 GGACC BmyI 960 GGGCAC G[AGT]GC[ACT]^C 982 GTGCAC BsaI 337 GAGACC GGTCTC(1/5) 71 GGTCTC Bsp1286I 960 GGGCAC G[AGT]GC[ACT]^C 982 GTGCAC BstXI 891 CCAAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1050 CCAAAAGCGTGG BstYI 426 GGATCT [GA]^GATC[CT] 1028 AGATCC Cac8I 31 GCCCGC GC[ACGT]^[ACGT]GC 532 GCTTGC Cfr13I 7 GGTCC G^G[ACGT]CC 1116 GGACC DsaI 422 CCACGG C^C[GA][CT]GG 815 CCATGG EarI 765 GAAGAG CTCTTC(1/4) 837 GAAGAG FauI 32 CCCGC CCCGC(4/6) 67 GCGGG HgaI 252 GACGC GACGC(5/10) 1194 GCGTC Ksp632I 765 GAAGAG CTCTTC(1/4) 837 GAAGAG MboII 765 GAAGA GAAGA(8/7) 837 GAAGA MflI 426 GGATCT [GA]^GATC[CT] 1028 AGATCC PaeR7I 539 CTCGAG C^TCGAG 722 CTCGAG Sau96I 7 GGTCC G^G[ACGT]CC 1116 GGACC SfcI 618 CTATAG C^T[GA][CT]AG 707 CTGCAG SmlI 539 CTCGAG C^T[CT][GA]AG 722 CTCGAG Tsp45I 194 GTCAC ^GT[GC]AC 599 GTGAC Tsp509I 747 AATT ^AATT 1111 AATT XbaI 429 TCTAGA T^CTAGA 484 TCTAGA XhoI 539 CTCGAG C^TCGAG 722 CTCGAG XhoII 426 GGATCT [GA]^GATC[CT] 1028 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI BalI BbvI BciVI BcnI BfaI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI BsrI BssKI BstF5I BstNI DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SfaNI TaqI TseI TspRI TthHB8I