Restriction map for
pFN21AE4402
(Circular) (Six-base) MAPSORT of: pFN21AE4402.seq Check: 4858 from: 1 to: 5956
pFN21AE4402 5956 bp
With 182 enzymes: SgfI *
March 28, 2012 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 4019 5403 5671 1632
Size: 986 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 986 268
AfeI AGC'GCT
Cuts at: 2155 2155
Size: 5956
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 2569 4855 5832 1203
Size: 1366 2286 977 1327
Fragments arranged by size:
2286 1366 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2245 1184
Size: 1061 4895
AlwNI CAG_nnn'CTG
Cuts at: 2648 3699 5271 2648
Size: 1051 1572 3333
Fragments arranged by size:
3333 1572 1051
ApaLI G'TGCA_C
Cuts at: 2987 4013 5169 2987
Size: 1026 1156 3774
Fragments arranged by size:
3774 1156 1026
ApoI r'AATT_y
Cuts at: 1493 2751 3265 3491 3643 5752 1493
Size: 1258 514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1258 514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2149 2544 2727 3281 1870
Size: 73 98 108 395 183 554 4545
Fragments arranged by size:
4545 554 395 183 108 98 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3277 4732 618
Size: 325 217 627 1490 1455 1842
Fragments arranged by size:
1842 1490 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3275 1391
Size: 1884 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2154 2282 2930 3848 5058 2154
Size: 128 648 918 1210 3052
Fragments arranged by size:
3052 1210 918 648 128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3008 3373 3008
Size: 365 5591
Bme1580I G_kGCm'C
Cuts at: 1790 2969 2991 4017 5173 1790
Size: 1179 22 1026 1156 2573
Fragments arranged by size:
2573 1179 1156 1026 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 2517 3059 3090 3224 4616 1239
Size: 1113 165 542 31 134 1392 2579
Fragments arranged by size:
2579 1392 1113 542 165 134 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4081 4946 5244 4081
Size: 865 298 4793
Fragments arranged by size:
4793 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 2336 4619 882
Size: 617 583 254 2283 2219
Fragments arranged by size:
2283 2219 617 583 254
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3199 3763 4145 4780
Size: 53 83 186 936 1666 564 382 635
Cuts at: 4780 275
Size: 1451
Fragments arranged by size:
1666 1451 936 635 564 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2104 4389 4787 5061 5208 5619 2104
Size: 2285 398 274 147 411 2441
Fragments arranged by size:
2441 2285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2037 2067 2903 2933 1178
Size: 30 165 30 634 30 836 30 4201
Fragments arranged by size:
4201 836 634 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2810 3087 1186
Size: 1168 456 277 4055
Fragments arranged by size:
4055 1168 456 277
BseYI C'CCAG_C
Cuts at: 2087 2209 2907 5159 5655 2087
Size: 122 698 2252 496 2388
Fragments arranged by size:
2388 2252 698 496 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2105 3406 4167 5195 1540
Size: 275 186 104 1301 761 1028 2301
Fragments arranged by size:
2301 1301 1028 761 275 186 104
BsiHKAI G_wGCw'C
Cuts at: 2991 4017 4102 5173 2991
Size: 1026 85 1071 3774
Fragments arranged by size:
3774 1071 1026 85
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2969 2991 3275 4017 4102 5173 1391
Size: 399 1179 22 284 742 85 1071 2174
Fragments arranged by size:
2174 1179 1071 742 399 284 85 22
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3311 844
Size: 2467 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2072 3843 1394
Size: 429 249 1771 3507
Fragments arranged by size:
3507 1771 429 249
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2745 4016 5028 2745
Size: 1271 1012 3673
Fragments arranged by size:
3673 1271 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2219 794
Size: 1425 4531
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 2603 1184
Size: 1419 4537
BstXI CCAn_nnnn'nTGG
Cuts at: 2903 3062 2903
Size: 159 5797
BstYI r'GATC_y
Cuts at: 1972 2431 3033 3286 3653 4038 4055 5507 5593
Size: 459 602 253 367 385 17 1452 86
Cuts at: 5593 5605 5951 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 602 459 385 367 346 253
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2427 2820 513
Size: 552 939 423 393 3649
Fragments arranged by size:
3649 939 552 423 393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3081 3472 4266 4286 3081
Size: 391 794 20 4751
Fragments arranged by size:
4751 794 391 20
ClaI AT'CG_AT
Cuts at: 1554 3165 1554
Size: 1611 4345
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DraIII CAC_nnn'GTG
Cuts at: 2591 2591
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2193 2296
Size: 54 1425 50 275 18 234 129 103
Cuts at: 2296 2503 2735 3403 4292 5921 8
Size: 207 232 668 889 1629 43
Fragments arranged by size:
1629 1425 889 668 275 234 232 207
129 103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2765 2837 3886 1353
Size: 1412 72 1049 3423
Fragments arranged by size:
3423 1412 1049 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2023 4543 4917 5063 387
Size: 1064 138 434 2520 374 146 1280
Fragments arranged by size:
2520 1280 1064 434 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2517 2618 3059 3090 3224 4019
Size: 393 720 165 101 441 31 134 795
Cuts at: 4019 4616 5403 5671 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 795 787 720 597 441 393 268
165 134 101 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2876 2876
Size: 5956
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
EcoRV GAT'ATC
Cuts at: 3220 3220
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2157 5103 1678
Size: 479 2946 2531
Fragments arranged by size:
2946 2531 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 2128 2160 2248
Size: 32 135 32 465 32 59 32 88
Cuts at: 2248 2280 2976 3008 4597 4629 4671 4703 1373
Size: 32 696 32 1589 32 42 32 2626
Fragments arranged by size:
2626 1589 696 465 135 88 59 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2125 2234 5070 5254 1305
Size: 820 109 2836 184 2007
Fragments arranged by size:
2836 2007 820 184 109
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2298 2737 10
Size: 54 1425 343 363 103 439 3229
Fragments arranged by size:
3229 1425 439 363 343 103 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2763 3914 4273 4432 518
Size: 648 1597 1151 359 159 2042
Fragments arranged by size:
2042 1597 1151 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 3372 4049 5197 5442 1606
Size: 481 1285 677 1148 245 2120
Fragments arranged by size:
2120 1285 1148 677 481 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2820 513
Size: 552 939 816 3649
Fragments arranged by size:
3649 939 816 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3257 3314 4859 1132
Size: 97 2028 57 1545 2229
Fragments arranged by size:
2229 2028 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2253 1108
Size: 1145 4811
PfoI T'CCnGG_A
Cuts at: 3036 3036
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspXI vC'TCGA_Gb
Cuts at: 2544 2544
Size: 5956
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2437 2469 2437
Size: 32 5924
PstI C_TGCA'G
Cuts at: 830 2716 3308 830
Size: 1886 592 3478
Fragments arranged by size:
3478 1886 592
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SapI GCTCTTCn'nnn_
Cuts at: 2765 2765
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 2623 2712 3304 4439 5120 5311 826
Size: 219 1578 89 592 1135 681 191 1471
Fragments arranged by size:
1578 1471 1135 681 592 219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 2043 3283 1872
Size: 171 1240 4545
Fragments arranged by size:
4545 1240 171
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2544 2727 4060 4961 5223 820
Size: 197 926 601 183 1333 901 262 1553
Fragments arranged by size:
1553 1333 926 901 601 262 197 183
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2871 1517
Size: 1354 4602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2820 3395 513
Size: 552 939 816 575 3074
Fragments arranged by size:
3074 939 816 575 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2260 2776 3138 3983 4000 4153 4338
Size: 1255 680 516 362 845 17 153 185
Cuts at: 4338 4783 325
Size: 445 1498
Fragments arranged by size:
1498 1255 845 680 516 445 362 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2903 2935 3225 3257 5916 5948 2903
Size: 32 290 32 2659 32 2911
Fragments arranged by size:
2911 2659 290 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2523 1316
Size: 1207 4749
XbaI T'CTAG_A
Cuts at: 2434 2489 3292 2434
Size: 55 803 5098
Fragments arranged by size:
5098 803 55
XhoI C'TCGA_G
Cuts at: 1943 2544 2727 1943
Size: 601 183 5172
Fragments arranged by size:
5172 601 183
XmaI C'CCGG_G
Cuts at: 1870 2041 3281 1870
Size: 171 1240 4545
Fragments arranged by size:
4545 1240 171
XmnI GAAnn'nnTTC
Cuts at: 2774 4085 2774
Size: 1311 4645
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI