(Linear) (Six-base) MAPSORT of: KIEE4402.seq Check: 5562 from: 1 to: 1251 KIEE4402.orf With 182 enzymes: SgfI * March 22, 2012 17:54 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 1251 Size: 613 638 AfeI AGC'GCT Cuts at: 0 150 1251 Size: 150 1101 AflIII A'CryG_T Cuts at: 0 564 1251 Size: 564 687 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 240 1251 Size: 240 1011 AlwNI CAG_nnn'CTG Cuts at: 0 643 1251 Size: 643 608 ApaLI G'TGCA_C Cuts at: 0 982 1251 Size: 982 269 ApoI r'AATT_y Cuts at: 0 746 1251 Size: 746 505 AvaI C'yCGr_G Cuts at: 0 36 144 539 722 1251 Size: 36 108 395 183 529 Fragments arranged by size: 529 395 183 108 36 BciVI GTATCCnnnnn_n' Cuts at: 0 149 277 925 1251 Size: 149 128 648 326 Fragments arranged by size: 648 326 149 128 BlpI GC'TnA_GC Cuts at: 0 1003 1251 Size: 1003 248 Bme1580I G_kGCm'C Cuts at: 0 964 986 1251 Size: 964 22 265 Fragments arranged by size: 964 265 22 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 347 512 1054 1085 1219 1251 Size: 347 165 542 31 134 32 Fragments arranged by size: 542 347 165 134 32 31 BsaI GGTCTCn'nnnn_ Cuts at: 0 77 331 1251 Size: 77 254 920 Fragments arranged by size: 920 254 77 BsaHI Gr'CG_yC Cuts at: 0 1194 1251 Size: 1194 57 BsaWI w'CCGG_w Cuts at: 0 99 1251 Size: 99 1152 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 32 62 898 928 1251 Size: 32 30 836 30 323 Fragments arranged by size: 836 323 32 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 349 805 1082 1251 Size: 349 456 277 169 Fragments arranged by size: 456 349 277 169 BseYI C'CCAG_C Cuts at: 0 82 204 902 1251 Size: 82 122 698 349 Fragments arranged by size: 698 349 122 82 BsiEI CG_ry'CG Cuts at: 0 100 1251 Size: 100 1151 BsiHKAI G_wGCw'C Cuts at: 0 986 1251 Size: 986 265 Bsp1286I G_dGCh'C Cuts at: 0 964 986 1251 Size: 964 22 265 Fragments arranged by size: 964 265 22 BsrBI CCG'CTC Cuts at: 0 67 1251 Size: 67 1184 BssSI C'ACGA_G Cuts at: 0 740 1251 Size: 740 511 BstAPI GCAn_nnn'nTGC Cuts at: 0 214 1251 Size: 214 1037 BstEII G'GTnAC_C Cuts at: 0 598 1251 Size: 598 653 BstXI CCAn_nnnn'nTGG Cuts at: 0 898 1057 1251 Size: 898 159 194 Fragments arranged by size: 898 194 159 BstYI r'GATC_y Cuts at: 0 426 1028 1251 Size: 426 602 223 Fragments arranged by size: 602 426 223 BtgI C'CryG_G Cuts at: 0 422 815 1251 Size: 422 393 436 Fragments arranged by size: 436 422 393 BtsI GCAGTG_nn' Cuts at: 0 1076 1251 Size: 1076 175 ClaI AT'CG_AT Cuts at: 0 1160 1251 Size: 1160 91 DraIII CAC_nnn'GTG Cuts at: 0 586 1251 Size: 586 665 EaeI y'GGCC_r Cuts at: 0 59 188 291 498 730 1251 Size: 59 129 103 207 232 521 Fragments arranged by size: 521 232 207 129 103 59 EarI CTCTTCn'nnn_ Cuts at: 0 760 832 1251 Size: 760 72 419 Fragments arranged by size: 760 419 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 18 1251 Size: 18 1233 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 347 512 613 1054 1085 1219 1251 Size: 347 165 101 441 31 134 32 Fragments arranged by size: 441 347 165 134 101 32 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 871 1251 Size: 871 380 EcoRV GAT'ATC Cuts at: 0 1215 1251 Size: 1215 36 HaeII r_GCGC'y Cuts at: 0 152 1251 Size: 152 1099 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 32 64 123 155 243 275 971 1003 Size: 32 32 59 32 88 32 696 32 Cuts at: 1003 1251 Size: 248 Fragments arranged by size: 696 248 88 59 32 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 120 229 1251 Size: 120 109 1022 Fragments arranged by size: 1022 120 109 MscI TGG'CCA Cuts at: 0 190 293 732 1251 Size: 190 103 439 519 Fragments arranged by size: 519 439 190 103 MslI CAynn'nnrTG Cuts at: 0 758 1251 Size: 758 493 MspA1I CmG'CkG Cuts at: 0 82 1251 Size: 82 1169 NcoI C'CATG_G Cuts at: 0 815 1251 Size: 815 436 PflMI CCAn_nnn'nTGG Cuts at: 0 248 1251 Size: 248 1003 PfoI T'CCnGG_A Cuts at: 0 1031 1251 Size: 1031 220 PspXI vC'TCGA_Gb Cuts at: 0 539 1251 Size: 539 712 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 432 464 1251 Size: 432 32 787 Fragments arranged by size: 787 432 32 PstI C_TGCA'G Cuts at: 0 711 1251 Size: 711 540 SapI GCTCTTCn'nnn_ Cuts at: 0 760 1251 Size: 760 491 SfcI C'TryA_G Cuts at: 0 618 707 1251 Size: 618 89 544 Fragments arranged by size: 618 544 89 SmaI CCC'GGG Cuts at: 0 38 1251 Size: 38 1213 SmlI C'TyrA_G Cuts at: 0 539 722 1251 Size: 539 183 529 Fragments arranged by size: 539 529 183 StuI AGG'CCT Cuts at: 0 866 1251 Size: 866 385 StyI C'CwwG_G Cuts at: 0 815 1251 Size: 815 436 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 255 771 1133 1251 Size: 255 516 362 118 Fragments arranged by size: 516 362 255 118 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 898 930 1220 1251 Size: 898 32 290 31 Fragments arranged by size: 898 290 32 31 Tth111I GACn'n_nGTC Cuts at: 0 518 1251 Size: 518 733 XbaI T'CTAG_A Cuts at: 0 429 484 1251 Size: 429 55 767 Fragments arranged by size: 767 429 55 XhoI C'TCGA_G Cuts at: 0 539 722 1251 Size: 539 183 529 Fragments arranged by size: 539 529 183 XmaI C'CCGG_G Cuts at: 0 36 1251 Size: 36 1215 XmnI GAAnn'nnTTC Cuts at: 0 769 1251 Size: 769 482 Enzymes that do cut: AcuI AfeI AflIII AlfI AlwNI ApaLI ApoI AvaI BciVI BlpI Bme1580I BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraIII EaeI EarI EciI Eco57MI EcoNI EcoRV HaeII Hin4I MmeI MscI MslI MspA1I NcoI PflMI PfoI PspXI PsrI PstI SapI SfcI SmaI SmlI StuI StyI TaqII TstI Tth111I XbaI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI CspCI DraI DrdI EagI EcoICRI EcoO109I EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI XcmI ZraI