(Linear) (Six-base) MAPSORT of: KIEE4331.seq Check: 9984 from: 1 to: 1014 KIEE4331.orf With 182 enzymes: SgfI * July 3, 2012 18:58 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 151 571 988 1014 Size: 33 118 420 417 26 Fragments arranged by size: 420 417 118 33 26 AfeI AGC'GCT Cuts at: 0 30 1014 Size: 30 984 AleI CACnn'nnGTG Cuts at: 0 358 706 1014 Size: 358 348 308 Fragments arranged by size: 358 348 308 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 322 354 1014 Size: 322 32 660 Fragments arranged by size: 660 322 32 AlwNI CAG_nnn'CTG Cuts at: 0 736 1014 Size: 736 278 ApaLI G'TGCA_C Cuts at: 0 633 1014 Size: 633 381 AvaI C'yCGr_G Cuts at: 0 326 569 961 1014 Size: 326 243 392 53 Fragments arranged by size: 392 326 243 53 BamHI G'GATC_C Cuts at: 0 561 686 1014 Size: 561 125 328 Fragments arranged by size: 561 328 125 BanI G'GyrC_C Cuts at: 0 763 1014 Size: 763 251 BbeI G_GCGC'C Cuts at: 0 767 1014 Size: 767 247 BbsI GAAGACnn'nnnn_ Cuts at: 0 613 768 1009 1014 Size: 613 155 241 5 Fragments arranged by size: 613 241 155 5 BglII A'GATC_T Cuts at: 0 198 1014 Size: 198 816 Bme1580I G_kGCm'C Cuts at: 0 387 637 1014 Size: 387 250 377 Fragments arranged by size: 387 377 250 BmrI ACTGGGnnnn_n' Cuts at: 0 689 1014 Size: 689 325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 320 1014 Size: 119 201 694 Fragments arranged by size: 694 201 119 BsaI GGTCTCn'nnnn_ Cuts at: 0 208 1014 Size: 208 806 BsaHI Gr'CG_yC Cuts at: 0 764 1014 Size: 764 250 BsaWI w'CCGG_w Cuts at: 0 426 1014 Size: 426 588 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 915 949 1014 Size: 915 34 65 Fragments arranged by size: 915 65 34 BseYI C'CCAG_C Cuts at: 0 386 601 1014 Size: 386 215 413 Fragments arranged by size: 413 386 215 BsiHKAI G_wGCw'C Cuts at: 0 637 1014 Size: 637 377 Bsp1286I G_dGCh'C Cuts at: 0 387 637 1014 Size: 387 250 377 Fragments arranged by size: 387 377 250 BsrDI GCAATG_nn' Cuts at: 0 416 800 1014 Size: 416 384 214 Fragments arranged by size: 416 384 214 BsrFI r'CCGG_y Cuts at: 0 697 760 1014 Size: 697 63 254 Fragments arranged by size: 697 254 63 BsrGI T'GTAC_A Cuts at: 0 479 1014 Size: 479 535 BstAPI GCAn_nnn'nTGC Cuts at: 0 736 1014 Size: 736 278 BstXI CCAn_nnnn'nTGG Cuts at: 0 360 1014 Size: 360 654 BstYI r'GATC_y Cuts at: 0 198 561 686 1014 Size: 198 363 125 328 Fragments arranged by size: 363 328 198 125 BtgI C'CryG_G Cuts at: 0 749 1014 Size: 749 265 BtsI GCAGTG_nn' Cuts at: 0 390 1014 Size: 390 624 EaeI y'GGCC_r Cuts at: 0 191 362 594 1014 Size: 191 171 232 420 Fragments arranged by size: 420 232 191 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 28 699 1014 Size: 28 671 315 Fragments arranged by size: 671 315 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 33 119 151 320 571 988 1014 Size: 33 86 32 169 251 417 26 Fragments arranged by size: 417 251 169 86 33 32 26 EcoO109I rG'GnC_Cy Cuts at: 0 92 1014 Size: 92 922 EcoRV GAT'ATC Cuts at: 0 171 1014 Size: 171 843 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 666 1014 Size: 666 348 HaeII r_GCGC'y Cuts at: 0 32 767 1014 Size: 32 735 247 Fragments arranged by size: 735 247 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 563 595 1014 Size: 563 32 419 Fragments arranged by size: 563 419 32 HincII GTy'rAC Cuts at: 0 576 1014 Size: 576 438 KasI G'GCGC_C Cuts at: 0 763 1014 Size: 763 251 MscI TGG'CCA Cuts at: 0 364 596 1014 Size: 364 232 418 Fragments arranged by size: 418 364 232 MslI CAynn'nnrTG Cuts at: 0 358 706 1014 Size: 358 348 308 Fragments arranged by size: 358 348 308 MspA1I CmG'CkG Cuts at: 0 226 255 376 666 873 1014 Size: 226 29 121 290 207 141 Fragments arranged by size: 290 226 207 141 121 29 NaeI GCC'GGC Cuts at: 0 699 762 1014 Size: 699 63 252 Fragments arranged by size: 699 252 63 NarI GG'CG_CC Cuts at: 0 764 1014 Size: 764 250 NcoI C'CATG_G Cuts at: 0 749 1014 Size: 749 265 NgoMIV G'CCGG_C Cuts at: 0 697 760 1014 Size: 697 63 254 Fragments arranged by size: 697 254 63 PfoI T'CCnGG_A Cuts at: 0 689 1014 Size: 689 325 PspXI vC'TCGA_Gb Cuts at: 0 569 1014 Size: 569 445 PvuII CAG'CTG Cuts at: 0 255 666 1014 Size: 255 411 348 Fragments arranged by size: 411 348 255 SfoI GGC'GCC Cuts at: 0 765 1014 Size: 765 249 SmaI CCC'GGG Cuts at: 0 328 1014 Size: 328 686 SmlI C'TyrA_G Cuts at: 0 569 1014 Size: 569 445 StuI AGG'CCT Cuts at: 0 973 1014 Size: 973 41 StyI C'CwwG_G Cuts at: 0 143 641 749 1014 Size: 143 498 108 265 Fragments arranged by size: 498 265 143 108 TatI w'GTAC_w Cuts at: 0 479 1014 Size: 479 535 Tth111I GACn'n_nGTC Cuts at: 0 520 1014 Size: 520 494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 747 1014 Size: 747 267 XhoI C'TCGA_G Cuts at: 0 569 1014 Size: 569 445 XmaI C'CCGG_G Cuts at: 0 326 1014 Size: 326 688 Enzymes that do cut: AcuI AfeI AleI AloI AlwNI ApaLI AvaI BamHI BanI BbeI BbsI BglII Bme1580I BmrI BpmI BsaI BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI EaeI EciI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PfoI PspXI PvuII SfoI SmaI SmlI StuI StyI TatI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI BanII BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI XbaI XmnI ZraI