################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 60 GGTACC G^GTACC AlwNI 790 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 964 GGGCCC GGGCC^C Asp718I 60 GGTACC G^GTACC AvaI 943 CTCGGG C^[CT]CG[GA]G AvrII 772 CCTAGG C^CTAGG BanI 60 GGTACC G^G[CT][GA]CC BanII 964 GGGCCC G[GA]GC[CT]^C BfaI 773 CTAG C^TAG BlnI 772 CCTAGG C^CTAGG BmyI 964 GGGCCC G[AGT]GC[ACT]^C BpmI 541 CTGGAG CTGGAG(16/14) BseRI 712 CTCCTC GAGGAG(10/8) BsoBI 943 CTCGGG C^[CT]CG[GA]G Bsp1286I 964 GGGCCC G[AGT]GC[ACT]^C BspMI 126 ACCTGC ACCTGC(4/8) BsrBI 909 GAGCGG CCGCTC(-3/-3) BsrDI 891 CATTGC GCAATG(2/0) BstEII 247 GGTGACC G^GT[ACGT]ACC BstPI 247 GGTGACC G^GT[ACGT]ACC DpnII 589 GATC ^GATC DsaI 547 CCATGG C^C[GA][CT]GG EaeI 668 TGGCCG [CT]^GGCC[GA] EarI 782 CTCTTC CTCTTC(1/4) EcoO65I 247 GGTGACC G^GT[ACGT]ACC EcoT22I 195 ATGCAT ATGCA^T FauI 951 CCCGC CCCGC(4/6) KpnI 60 GGTACC GGTAC^C Ksp632I 782 CTCTTC CTCTTC(1/4) MaeI 773 CTAG C^TAG MboI 589 GATC ^GATC MseI 95 TTAA T^TAA MslI 675 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 933 CAGCTG C[AC]G^C[GT]G NcoI 547 CCATGG C^CATGG NdeI 382 CATATG CA^TATG NdeII 589 GATC ^GATC NsiI 195 ATGCAT ATGCA^T PstI 936 CTGCAG CTGCA^G PvuII 933 CAGCTG CAG^CTG Sau3AI 589 GATC ^GATC ScaI 363 AGTACT AGT^ACT TaqI 573 TCGA T^CGA Tru9I 95 TTAA T^TAA TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 573 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 952 CCGC CCGC(-3/-1) 911 GCGG Asp700I 83 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 493 GAAAAGTTTC BsmFI 946 GGGAC GGGAC(10/14) 652 GTCCC BsmI 350 GAATGC GAATGC(1/-1) 288 GCATTC DrdI 520 GACTTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 643 GACGAAACGGTC EcoRI 87 GAATTC G^AATTC 777 GAATTC MwoI 11 GCCTTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 271 GCTGAATTTGC PpuMI 527 AGGTCCT [GA]G^G[AT]CC[CT] 946 GGGACCC SfcI 112 CTACAG C^T[GA][CT]AG 936 CTGCAG SmlI 228 CTCAAG C^T[CT][GA]AG 258 CTTGAG TfiI 373 GATTC G^A[AT]TC 921 GAATC XmnI 83 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 493 GAAAAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AcsI AfaI AluI ApoI AvaII BbsI BbvI BpuAI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MvaI NlaIII NlaIV PleI RsaI Sau96I ScrFI StyI TseI Tsp45I Tsp509I