################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcyI 852 GACGCC G[GA]^CG[CT]C Alw44I 1039 GTGCAC G^TGCAC AlwNI 964 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1039 GTGCAC G^TGCAC Asp700I 315 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1011 CTCGGG C^[CT]CG[GA]G BbsI 174 GAAGAC GAAGAC(2/6) BglII 23 AGATCT A^GATCT BlpI 386 GCTCAGC GC^T[ACGT]AGC Bpu1102I 386 GCTCAGC GC^T[ACGT]AGC BpuAI 174 GAAGAC GAAGAC(2/6) BsaHI 852 GACGCC G[GA]^CG[CT]C BsaI 624 GGTCTC GGTCTC(1/5) BsaWI 374 ACCGGA [AT]^CCGG[AT] BsgI 262 CTGCAC GTGCAG(16/14) BsmAI 625 GTCTC GTCTC(1/5) BsoBI 1011 CTCGGG C^[CT]CG[GA]G Bsp1407I 1052 TGTACA T^GTACA BsrBI 11 CCGCTC CCGCTC(-3/-3) BsrGI 1052 TGTACA T^GTACA BssSI 1156 CACGAG CACGAG(-5/-1) BstXI 825 CCACGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 386 GCTCAGC GC^T[ACGT]AGC DsaI 1082 CCATGG C^C[GA][CT]GG EarI 349 CTCTTC CTCTTC(1/4) EcoNI 978 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 852 GACGC GACGC(5/10) Hin1I 852 GACGCC G[GA]^CG[CT]C HindIII 37 AAGCTT A^AGCTT Ksp632I 349 CTCTTC CTCTTC(1/4) NcoI 1082 CCATGG C^CATGG NspI 191 ACATGC [GA]CATG^[CT] PleI 897 GAGTC GAGTC(4/5) PshAI 618 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SmlI 733 CTCAAG C^T[CT][GA]AG SspBI 1052 TGTACA T^GTACA Tsp509I 697 AATT ^AATT XmnI 315 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 814 GTCTAC GT^[AC][GT]AC 899 GTCTAC AfaI 1053 GTAC GT^AC 1140 GTAC AlwI 1104 GATCC GGATC(4/5) 1134 GATCC AspHI 514 GTGCTC G[AT]GC[AT]^C 1039 GTGCAC AspI 469 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 893 GACAGAGTC BsiHKAI 514 GTGCTC G[AT]GC[AT]^C 1039 GTGCAC BspMI 107 ACCTGC ACCTGC(4/8) 339 GCAGGT BsrFI 446 GCCGGC [GA]^CCGG[CT] 487 GCCGGC BssHII 558 GCGCGC G^CGCGC 560 GCGCGC BstAPI 963 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1016 GCACCGCTTGC BstYI 23 AGATCT [GA]^GATC[CT] 1133 AGATCC Cfr10I 446 GCCGGC [GA]^CCGG[CT] 487 GCCGGC EaeI 2 TGGCCG [CT]^GGCC[GA] 635 TGGCCG EcoT14I 52 CCTTGG C^C[AT][AT]GG 1082 CCATGG MaeIII 616 GTGAC ^GT[ACGT]AC 850 GTGAC MflI 23 AGATCT [GA]^GATC[CT] 1133 AGATCC NaeI 446 GCCGGC GCC^GGC 487 GCCGGC NgoMIV 446 GCCGGC G^CCGGC 487 GCCGGC PflFI 469 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 893 GACAGAGTC PpuMI 473 GGGTCCC [GA]G^G[AT]CC[CT] 752 AGGTCCT PvuII 112 CAGCTG CAG^CTG 298 CAGCTG RsaI 1053 GTAC GT^AC 1140 GTAC StyI 52 CCTTGG C^C[AT][AT]GG 1082 CCATGG TaqI 416 TCGA T^CGA 455 TCGA Tsp45I 616 GTGAC ^GT[GC]AC 850 GTGAC TspRI 587 CAGTG CA[GC]TG[ACGT][ACGT]^ 1037 CAGTG Tth111I 469 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 893 GACAGAGTC TthHB8I 416 TCGA T^CGA 455 TCGA XhoII 23 AGATCT [GA]^GATC[CT] 1133 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AluI ApaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI TseI