(Circular) (Six-base) MAPSORT of: pFN21AE4374.seq Check: 1633 from: 1 to: 5881 pFN21AE4374 5881 bp With 182 enzymes: SgfI * March 28, 2012 14:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2335 2335 Size: 5881 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 2905 3224 1937 Size: 883 85 319 4594 Fragments arranged by size: 4594 883 319 85 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3152 3944 5328 5596 1632 Size: 1520 792 1384 268 1917 Fragments arranged by size: 1917 1520 1384 792 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 2795 1166 Size: 1629 4252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2974 3624 5196 2974 Size: 650 1572 3659 Fragments arranged by size: 3659 1572 650 ApaI G_GGCC'C Cuts at: 2712 2778 2983 2712 Size: 66 205 5610 Fragments arranged by size: 5610 205 66 ApaLI G'TGCA_C Cuts at: 3044 3938 5094 3044 Size: 894 1156 3831 Fragments arranged by size: 3831 1156 894 ApoI r'AATT_y Cuts at: 1493 3190 3416 3568 5677 1493 Size: 1697 226 152 2109 1697 Fragments arranged by size: 2109 1697 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 3016 3206 1870 Size: 73 1073 190 4545 Fragments arranged by size: 4545 1073 190 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 2597 3020 3202 4657 Size: 325 217 627 708 102 423 182 1455 Cuts at: 4657 618 Size: 1842 Fragments arranged by size: 1842 1455 708 627 423 325 217 182 102 BanII G_rGCy'C Cuts at: 1391 2166 2538 2712 2778 2983 3160 3200 1391 Size: 775 372 174 66 205 177 40 4072 Fragments arranged by size: 4072 775 372 205 177 174 66 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2186 928 Size: 971 287 4623 Fragments arranged by size: 4623 971 287 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4072 4106 1903 Size: 34 2135 34 3678 Fragments arranged by size: 3678 2135 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3773 4983 3773 Size: 1210 4671 BclI T'GATC_A Cuts at: 1548 1548 Size: 5881 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4492 136 Size: 107 122 71 631 605 2820 1525 Fragments arranged by size: 2820 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2028 5876 2028 Size: 3848 2033 BlpI GC'TnA_GC Cuts at: 2392 3298 2392 Size: 906 4975 Bme1580I G_kGCm'C Cuts at: 1790 2038 2712 2778 2983 3023 3048 3942 5098 Size: 248 674 66 205 40 25 894 1156 Cuts at: 5098 1790 Size: 2573 Fragments arranged by size: 2573 1156 894 674 248 205 66 40 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2485 2585 3035 Size: 416 53 83 399 24 1068 100 450 Cuts at: 3035 3612 4570 442 Size: 577 958 1753 Fragments arranged by size: 1753 1068 958 577 450 416 399 100 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2266 2450 2459 2631 2867 2907 3005 Size: 838 189 184 9 172 236 40 98 Cuts at: 3005 4541 1239 Size: 1536 2579 Fragments arranged by size: 2579 1536 838 236 189 184 172 98 40 9 Bpu10I CC'TnA_GC Cuts at: 3102 3102 Size: 5881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 4006 4871 5169 2723 Size: 1283 865 298 3435 Fragments arranged by size: 3435 1283 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2635 4544 882 Size: 617 1136 1909 2219 Fragments arranged by size: 2219 1909 1136 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2858 3688 4070 4705 Size: 53 83 186 936 1325 830 382 635 Cuts at: 4705 275 Size: 1451 Fragments arranged by size: 1451 1325 936 830 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2379 4314 4712 4986 5133 5544 2379 Size: 1935 398 274 147 411 2716 Fragments arranged by size: 2716 1935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2261 2291 2604 2634 3128 Size: 30 165 30 858 30 313 30 494 Cuts at: 3128 3158 1178 Size: 30 3901 Fragments arranged by size: 3901 858 494 313 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 2269 3002 1186 Size: 894 189 733 4065 Fragments arranged by size: 4065 894 733 189 BseYI C'CCAG_C Cuts at: 2226 2272 2542 2647 5084 5580 2226 Size: 46 270 105 2437 496 2527 Fragments arranged by size: 2527 2437 496 270 105 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 1163 Size: 573 516 4792 Fragments arranged by size: 4792 573 516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2523 3048 3942 4027 5098 2523 Size: 525 894 85 1071 3306 Fragments arranged by size: 3306 1071 894 525 85 BsmI GAATG_Cn' Cuts at: 3392 3485 3392 Size: 93 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2166 2523 2538 2712 2778 2983 Size: 399 248 128 357 15 174 66 205 Cuts at: 2983 3023 3048 3160 3200 3942 4027 5098 1391 Size: 40 25 112 40 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 357 248 205 174 128 112 85 66 40 40 25 15 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2121 2335 3236 844 Size: 1277 214 901 3489 Fragments arranged by size: 3489 1277 901 214 BsrBI CCG'CTC Cuts at: 1394 1823 2018 3768 1394 Size: 429 195 1750 3507 Fragments arranged by size: 3507 1750 429 195 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 4550 65 Size: 1183 3128 174 1396 Fragments arranged by size: 3128 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2451 2492 4525 4712 1700 Size: 110 641 41 2033 187 2869 Fragments arranged by size: 2869 2033 641 187 110 41 BsrGI T'GTAC_A Cuts at: 96 3057 96 Size: 2961 2920 BssHII G'CGCG_C Cuts at: 1925 2563 2565 1925 Size: 638 2 5241 Fragments arranged by size: 5241 638 2 BssSI C'ACGA_G Cuts at: 3161 3941 4953 3161 Size: 780 1012 4089 Fragments arranged by size: 4089 1012 780 BstAPI GCAn_nnn'nTGC Cuts at: 794 2974 3027 794 Size: 2180 53 3648 Fragments arranged by size: 3648 2180 53 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstXI CCAn_nnnn'nTGG Cuts at: 2837 2837 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2028 3138 3211 3578 3963 3980 5432 5518 Size: 56 1110 73 367 385 17 1452 86 Cuts at: 5518 5530 5876 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1110 385 367 346 86 73 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3087 513 Size: 552 939 1083 3307 Fragments arranged by size: 3307 1083 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 3397 4191 4211 3397 Size: 794 20 5067 Fragments arranged by size: 5067 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2677 2712 529 Size: 35 2113 35 3698 Fragments arranged by size: 3698 2113 35 35 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2640 3328 Size: 54 1425 50 275 18 177 633 688 Cuts at: 3328 4217 5846 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 688 633 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 3811 1353 Size: 1007 1451 3423 Fragments arranged by size: 3423 1451 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2348 2438 4468 4842 4988 387 Size: 1064 138 759 90 2030 374 146 1280 Fragments arranged by size: 2030 1280 1064 759 374 146 138 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 2266 2450 2459 2631 2867 2907 Size: 393 445 189 184 9 172 236 40 Cuts at: 2907 3005 3152 3944 4541 5328 5596 1239 Size: 98 147 792 597 787 268 1524 Fragments arranged by size: 1524 792 787 597 445 393 268 236 189 184 172 147 98 40 9 EcoNI CCTnn'n_nnAGG Cuts at: 2987 2987 Size: 5881 EcoO109I rG'GnC_Cy Cuts at: 2479 2758 2980 2479 Size: 279 222 5380 Fragments arranged by size: 5380 279 222 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4522 4554 4596 4628 1373 Size: 32 135 32 2950 32 42 32 2626 Fragments arranged by size: 2950 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 2042 748 Size: 1294 4587 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4995 5179 1305 Size: 3690 184 2007 Fragments arranged by size: 3690 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4059 Fragments arranged by size: 4059 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2462 2795 2835 3839 4198 4357 Size: 648 1035 261 333 40 1004 359 159 Cuts at: 4357 518 Size: 2042 Fragments arranged by size: 2042 1035 1004 648 359 333 261 159 40 MspA1I CmG'CkG Cuts at: 1606 2119 2305 2866 3297 3974 5122 5367 1606 Size: 513 186 561 431 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 561 513 431 245 186 NaeI GCC'GGC Cuts at: 2453 2494 2453 Size: 41 5840 NcoI C'CATG_G Cuts at: 513 1065 2004 3087 513 Size: 552 939 1083 3307 Fragments arranged by size: 3307 1083 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NgoMIV G'CCGG_C Cuts at: 2451 2492 2451 Size: 41 5840 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 2200 3239 4784 1132 Size: 97 971 1039 1545 2229 Fragments arranged by size: 2229 1545 1039 971 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2522 2554 2745 2777 3155 3187 3950 3982 5463 Size: 32 191 32 378 32 763 32 1481 Cuts at: 5463 5495 2522 Size: 32 2908 Fragments arranged by size: 2908 1481 763 378 191 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2479 2758 2479 Size: 279 5602 PshAI GACnn'nnGTC Cuts at: 1613 2627 1613 Size: 1014 4867 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PspOMI G'GGCC_C Cuts at: 2708 2774 2979 2708 Size: 66 205 5610 Fragments arranged by size: 5610 205 66 PstI C_TGCA'G Cuts at: 830 2110 2118 2304 3233 830 Size: 1280 8 186 929 3478 Fragments arranged by size: 3478 1280 929 186 8 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2119 2305 2119 Size: 186 5695 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SanDI GG'GwC_CC Cuts at: 2479 2479 Size: 5881 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2106 2114 2300 3229 4364 5045 5236 Size: 219 1061 8 186 929 1135 681 191 Cuts at: 5236 826 Size: 1471 Fragments arranged by size: 1471 1135 1061 929 681 219 191 186 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2738 3985 4886 5148 820 Size: 197 926 795 1247 901 262 1553 Fragments arranged by size: 1553 1247 926 901 795 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2004 2057 3087 3320 513 Size: 552 939 53 1030 233 3074 Fragments arranged by size: 3074 1030 939 552 233 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2286 2534 2585 2656 3908 3925 4078 Size: 1255 706 248 51 71 1252 17 153 Cuts at: 4078 4263 4708 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1252 706 445 248 185 153 71 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3057 4127 5839 Size: 274 80 33 51 1443 1080 1070 1712 Cuts at: 5839 96 Size: 138 Fragments arranged by size: 1712 1443 1080 1070 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2261 2293 2604 2636 5841 5873 2261 Size: 32 311 32 3205 32 2269 Fragments arranged by size: 3205 2269 311 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2477 2901 1316 Size: 1161 424 4296 Fragments arranged by size: 4296 1161 424 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 2324 4010 2324 Size: 1686 4195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI