(Linear) (Six-base) MAPSORT of: KIEE4374.seq Check: 6317 from: 1 to: 1176 KIEE4374.orf With 182 enzymes: SgfI * March 22, 2012 17:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 330 1176 Size: 330 846 AccI GT'mk_AC Cuts at: 0 815 900 1176 Size: 815 85 276 Fragments arranged by size: 815 276 85 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1147 1176 Size: 1147 29 AleI CACnn'nnGTG Cuts at: 0 790 1176 Size: 790 386 AlwNI CAG_nnn'CTG Cuts at: 0 969 1176 Size: 969 207 ApaI G_GGCC'C Cuts at: 0 707 773 978 1176 Size: 707 66 205 198 Fragments arranged by size: 707 205 198 66 ApaLI G'TGCA_C Cuts at: 0 1039 1176 Size: 1039 137 AvaI C'yCGr_G Cuts at: 0 1011 1176 Size: 1011 165 BanI G'GyrC_C Cuts at: 0 490 592 1015 1176 Size: 490 102 423 161 Fragments arranged by size: 490 423 161 102 BanII G_rGCy'C Cuts at: 0 161 533 707 773 978 1155 1176 Size: 161 372 174 66 205 177 21 Fragments arranged by size: 372 205 177 174 161 66 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 181 1176 Size: 181 995 BglII A'GATC_T Cuts at: 0 23 1176 Size: 23 1153 BlpI GC'TnA_GC Cuts at: 0 387 1176 Size: 387 789 Bme1580I G_kGCm'C Cuts at: 0 33 707 773 978 1018 1043 1176 Size: 33 674 66 205 40 25 133 Fragments arranged by size: 674 205 133 66 40 33 25 BmrI ACTGGGnnnn_n' Cuts at: 0 480 580 1030 1176 Size: 480 100 450 146 Fragments arranged by size: 480 450 146 100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 261 445 454 626 862 902 1000 Size: 72 189 184 9 172 236 40 98 Cuts at: 1000 1176 Size: 176 Fragments arranged by size: 236 189 184 176 172 98 72 40 9 Bpu10I CC'TnA_GC Cuts at: 0 1097 1176 Size: 1097 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1176 Size: 718 458 BsaI GGTCTCn'nnnn_ Cuts at: 0 630 1176 Size: 630 546 BsaHI Gr'CG_yC Cuts at: 0 853 1176 Size: 853 323 BsaWI w'CCGG_w Cuts at: 0 374 1176 Size: 374 802 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 256 286 599 629 1123 1153 1176 Size: 256 30 313 30 494 30 23 Fragments arranged by size: 494 313 256 30 30 30 23 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 75 264 997 1176 Size: 75 189 733 179 Fragments arranged by size: 733 189 179 75 BseYI C'CCAG_C Cuts at: 0 221 267 537 642 1176 Size: 221 46 270 105 534 Fragments arranged by size: 534 270 221 105 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 1176 Size: 247 929 BsiHKAI G_wGCw'C Cuts at: 0 518 1043 1176 Size: 518 525 133 Fragments arranged by size: 525 518 133 Bsp1286I G_dGCh'C Cuts at: 0 33 161 518 533 707 773 978 1018 Size: 33 128 357 15 174 66 205 40 Cuts at: 1018 1043 1155 1176 Size: 25 112 21 Fragments arranged by size: 357 205 174 128 112 66 40 33 25 21 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 116 330 1176 Size: 116 214 846 Fragments arranged by size: 846 214 116 BsrBI CCG'CTC Cuts at: 0 13 1176 Size: 13 1163 BsrFI r'CCGG_y Cuts at: 0 446 487 1176 Size: 446 41 689 Fragments arranged by size: 689 446 41 BsrGI T'GTAC_A Cuts at: 0 1052 1176 Size: 1052 124 BssHII G'CGCG_C Cuts at: 0 558 560 1176 Size: 558 2 616 Fragments arranged by size: 616 558 2 BssSI C'ACGA_G Cuts at: 0 1156 1176 Size: 1156 20 BstAPI GCAn_nnn'nTGC Cuts at: 0 969 1022 1176 Size: 969 53 154 Fragments arranged by size: 969 154 53 BstXI CCAn_nnnn'nTGG Cuts at: 0 832 1176 Size: 832 344 BstYI r'GATC_y Cuts at: 0 23 1133 1176 Size: 23 1110 43 Fragments arranged by size: 1110 43 23 BtgI C'CryG_G Cuts at: 0 1082 1176 Size: 1082 94 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 672 707 1176 Size: 672 35 469 Fragments arranged by size: 672 469 35 EaeI y'GGCC_r Cuts at: 0 2 635 1176 Size: 2 633 541 Fragments arranged by size: 633 541 2 EarI CTCTTCn'nnn_ Cuts at: 0 355 1176 Size: 355 821 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 343 433 1176 Size: 343 90 743 Fragments arranged by size: 743 343 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 261 445 454 626 862 902 1000 Size: 72 189 184 9 172 236 40 98 Cuts at: 1000 1147 1176 Size: 147 29 Fragments arranged by size: 236 189 184 172 147 98 72 40 29 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 982 1176 Size: 982 194 EcoO109I rG'GnC_Cy Cuts at: 0 474 753 975 1176 Size: 474 279 222 201 Fragments arranged by size: 474 279 222 201 HindIII A'AGCT_T Cuts at: 0 37 1176 Size: 37 1139 MslI CAynn'nnrTG Cuts at: 0 196 457 790 830 1176 Size: 196 261 333 40 346 Fragments arranged by size: 346 333 261 196 40 MspA1I CmG'CkG Cuts at: 0 114 300 861 1176 Size: 114 186 561 315 Fragments arranged by size: 561 315 186 114 NaeI GCC'GGC Cuts at: 0 448 489 1176 Size: 448 41 687 Fragments arranged by size: 687 448 41 NcoI C'CATG_G Cuts at: 0 1082 1176 Size: 1082 94 NgoMIV G'CCGG_C Cuts at: 0 446 487 1176 Size: 446 41 689 Fragments arranged by size: 689 446 41 NspI r_CATG'y Cuts at: 0 195 1176 Size: 195 981 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 517 549 740 772 1150 1176 Size: 517 32 191 32 378 26 Fragments arranged by size: 517 378 191 32 32 26 PpuMI rG'GwC_Cy Cuts at: 0 474 753 1176 Size: 474 279 423 Fragments arranged by size: 474 423 279 PshAI GACnn'nnGTC Cuts at: 0 622 1176 Size: 622 554 PspOMI G'GGCC_C Cuts at: 0 703 769 974 1176 Size: 703 66 205 202 Fragments arranged by size: 703 205 202 66 PstI C_TGCA'G Cuts at: 0 105 113 299 1176 Size: 105 8 186 877 Fragments arranged by size: 877 186 105 8 PvuII CAG'CTG Cuts at: 0 114 300 1176 Size: 114 186 876 Fragments arranged by size: 876 186 114 SanDI GG'GwC_CC Cuts at: 0 474 1176 Size: 474 702 SfcI C'TryA_G Cuts at: 0 101 109 295 1176 Size: 101 8 186 881 Fragments arranged by size: 881 186 101 8 SmlI C'TyrA_G Cuts at: 0 733 1176 Size: 733 443 StyI C'CwwG_G Cuts at: 0 52 1082 1176 Size: 52 1030 94 Fragments arranged by size: 1030 94 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 281 529 580 651 1176 Size: 281 248 51 71 525 Fragments arranged by size: 525 281 248 71 51 TatI w'GTAC_w Cuts at: 0 1052 1176 Size: 1052 124 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 256 288 599 631 1176 Size: 256 32 311 32 545 Fragments arranged by size: 545 311 256 32 32 Tth111I GACn'n_nGTC Cuts at: 0 472 896 1176 Size: 472 424 280 Fragments arranged by size: 472 424 280 XmnI GAAnn'nnTTC Cuts at: 0 319 1176 Size: 319 857 Enzymes that do cut: AarI AccI AcuI AleI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I HindIII MslI MspA1I NaeI NcoI NgoMIV NspI PpiI PpuMI PshAI PspOMI PstI PvuII SanDI SfcI SmlI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI ZraI