Restriction map for
pFN21AB3769
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AvrII
BamHI BbrPI BbvCI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssSI BstBI Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
EarI Eco47III Eco81I EcoRI EcoT22I
FseI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NsiI NspI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshBI PvuI RsrII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 1333 GTATAC GT^[AC][GT]AC
AclI 1468 AACGTT AA^CGTT
AcsI 232 AAATTC [GA]^AATT[CT]
AflIII 626 ACGTGT A^C[GA][CT]GT
AgeI 1492 ACCGGT A^CCGGT
Alw44I 418 GTGCAC G^TGCAC
ApaI 769 GGGCCC GGGCC^C
ApaLI 418 GTGCAC G^TGCAC
ApoI 232 AAATTC [GA]^AATT[CT]
AspI 1609 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 179 TGGCCA TGG^CCA
BbeI 1123 GGCGCC GGCGC^C
BcgI 851 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1034 GTATCC GTATCC(6/5)
BclI 1739 TGATCA T^GATCA
BglI 149 GCCTCATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 124 TACGTG [CT]AC^GT[GA]
BsaI 331 GAGACC GGTCTC(1/5)
BspHI 1235 TCATGA T^CATGA
BsrBI 1576 GAGCGG CCGCTC(-3/-3)
Bst1107I 1333 GTATAC GTA^TAC
BstEII 1434 GGTTACC G^GT[ACGT]ACC
BstPI 1434 GGTTACC G^GT[ACGT]ACC
BstXI 119 CCAGCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1333 GTATAC GTA^TAC
DrdI 1197 GACCCCGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 674 CGGCCG C^GGCCG
EclXI 674 CGGCCG C^GGCCG
Eco52I 674 CGGCCG C^GGCCG
EcoNI 729 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1434 GGTTACC G^GT[ACGT]ACC
EcoRV 357 GATATC GAT^ATC
FbaI 1739 TGATCA T^GATCA
KasI 1123 GGCGCC G^GCGCC
KspI 1127 CCGCGG CCGC^GG
MluNI 179 TGGCCA TGG^CCA
MscI 179 TGGCCA TGG^CCA
MseI 1466 TTAA T^TAA
MslI 375 CACGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 676 GCCGGC GCC^GGC
NarI 1123 GGCGCC GG^CGCC
NcoI 365 CCATGG C^CATGG
NgoMIV 676 GCCGGC G^CCGGC
NruI 1605 TCGCGA TCG^CGA
PflFI 1609 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1492 ACCGGT A^CCGGT
Psp1406I 1468 AACGTT AA^CGTT
PstI 1057 CTGCAG CTGCA^G
PvuII 1223 CAGCTG CAG^CTG
RcaI 1235 TCATGA T^CATGA
SacII 1127 CCGCGG CCGC^GG
SexAI 173 ACCAGGT A^CC[AT]GGT
SfcI 1057 CTGCAG C^T[GA][CT]AG
SfoI 1123 GGCGCC GGC^GCC
Tru9I 1466 TTAA T^TAA
Tsp45I 1195 GTGAC ^GT[GC]AC
Tsp509I 233 AATT ^AATT
Tth111I 1609 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwNI 1048 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1174 CAGGACCTG
AviII 512 TGCGCA TGC^GCA
797 TGCGCA
BbsI 1669 GAAGAC GAAGAC(2/6)
61 GTCTTC
BpmI 949 CTGGAG CTGGAG(16/14)
1633 CTGGAG
BpuAI 1669 GAAGAC GAAGAC(2/6)
61 GTCTTC
BsaWI 1492 ACCGGT [AT]^CCGG[AT]
1556 ACCGGA
BsmAI 331 GAGAC GTCTC(1/5)
1219 GAGAC
BstAPI 800 GCACCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1173 GCAGGACCTGC
FspI 512 TGCGCA TGC^GCA
797 TGCGCA
PshAI 1525 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1723 GACAAGTGTC
TfiI 1398 GATTC G^A[AT]TC
1602 GAATC
TspRI 248 CAGTG CA[GC]TG[ACGT][ACGT]^
268 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI TthHB8I
XcmI XhoII