(Circular) (Six-base) MAPSORT of: pFN21AB3769.seq Check: 1990 from: 1 to: 6622 pFN21AB3769 6622 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3339 3965 1937 Size: 1402 626 4594 Fragments arranged by size: 4594 1402 626 Acc65I G'GTAC_C Cuts at: 3943 3943 Size: 6622 AclI AA'CG_TT Cuts at: 1563 3474 4749 5122 1563 Size: 1911 1275 373 3063 Fragments arranged by size: 3063 1911 1275 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4685 6069 6337 1632 Size: 3053 1384 268 1917 Fragments arranged by size: 3053 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 2631 5521 6498 1203 Size: 1428 2890 977 1327 Fragments arranged by size: 2890 1428 1327 977 AgeI A'CCGG_T Cuts at: 3497 5453 3497 Size: 1956 4666 AhdI GACnn_n'nnGTC Cuts at: 5351 5351 Size: 6622 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6622 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6622 AlwNI CAG_nnn'CTG Cuts at: 3058 3184 4365 5937 3058 Size: 126 1181 1572 3743 Fragments arranged by size: 3743 1572 1181 126 ApaI G_GGCC'C Cuts at: 2778 2778 Size: 6622 ApaLI G'TGCA_C Cuts at: 2423 4679 5835 2423 Size: 2256 1156 3210 Fragments arranged by size: 3210 2256 1156 ApoI r'AATT_y Cuts at: 1493 2237 3931 4157 4309 6418 1493 Size: 744 1694 226 152 2109 1697 Fragments arranged by size: 2109 1697 1694 744 226 152 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 3947 1870 Size: 73 2004 4545 Fragments arranged by size: 4545 2004 73 BamHI G'GATC_C Cuts at: 3952 4319 3952 Size: 367 6255 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2492 3128 3943 5398 Size: 325 217 627 255 450 636 815 1455 Cuts at: 5398 618 Size: 1842 Fragments arranged by size: 1842 1455 815 636 627 450 325 255 217 BanII G_rGCy'C Cuts at: 1391 2508 2535 2778 2840 2928 3810 3941 1391 Size: 1117 27 243 62 88 882 131 4072 Fragments arranged by size: 4072 1117 882 243 131 88 62 27 BbeI G_GCGC'C Cuts at: 3132 3132 Size: 6622 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2059 3681 928 Size: 971 160 1622 3869 Fragments arranged by size: 3869 1622 971 160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2845 2879 4813 4847 1903 Size: 34 908 34 1934 34 3678 Fragments arranged by size: 3678 1934 908 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3050 4514 5724 3050 Size: 1464 1210 3948 Fragments arranged by size: 3948 1464 1210 BclI T'GATC_A Cuts at: 1548 3744 1548 Size: 2196 4426 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2160 5233 136 Size: 107 122 71 631 605 488 3073 1525 Fragments arranged by size: 3073 1525 631 605 488 122 107 71 BglII A'GATC_T Cuts at: 6617 6617 Size: 6622 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 2045 2427 2778 4683 5839 1790 Size: 255 382 351 1905 1156 2573 Fragments arranged by size: 2573 1905 1156 382 351 255 BmgBI CAC'GTC Cuts at: 1318 2632 3199 1318 Size: 1314 567 4741 Fragments arranged by size: 4741 1314 567 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4353 5311 442 Size: 416 53 83 399 24 2936 958 1753 Fragments arranged by size: 2936 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2884 2383 Size: 501 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2975 3659 5282 1239 Size: 1736 684 1623 2579 Fragments arranged by size: 2579 1736 1623 684 Bpu10I CC'TnA_GC Cuts at: 2834 2834 Size: 6622 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4747 5612 5910 4747 Size: 865 298 5459 Fragments arranged by size: 5459 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2330 5285 882 Size: 617 831 2955 2219 Fragments arranged by size: 2955 2219 831 617 BsaAI yAC'GTr Cuts at: 493 1204 2131 4413 493 Size: 711 927 2282 2702 Fragments arranged by size: 2702 2282 927 711 BsaBI GATnn'nnATC Cuts at: 6616 6616 Size: 6622 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2214 2982 3129 Size: 53 83 186 936 633 48 768 147 Cuts at: 3129 3456 4429 4811 5446 275 Size: 327 973 382 635 1451 Fragments arranged by size: 1451 973 936 768 635 633 382 327 186 147 83 53 48 BsaWI w'CCGG_w Cuts at: 3497 3561 5055 5453 5727 5874 6285 3497 Size: 64 1494 398 274 147 411 3834 Fragments arranged by size: 3834 1494 411 398 274 147 64 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6397 Fragments arranged by size: 6397 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2756 2936 1186 Size: 1186 384 180 4872 Fragments arranged by size: 4872 1186 384 180 BseYI C'CCAG_C Cuts at: 2123 3214 3379 5825 6321 2123 Size: 1091 165 2446 496 2424 Fragments arranged by size: 2446 2424 1091 496 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 3158 3197 3353 3590 1163 Size: 573 540 882 39 156 237 4195 Fragments arranged by size: 4195 882 573 540 237 156 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2682 3616 3760 4072 4833 5861 Size: 275 186 681 934 144 312 761 1028 Cuts at: 5861 1540 Size: 2301 Fragments arranged by size: 2301 1028 934 761 681 312 275 186 144 BsiHKAI G_wGCw'C Cuts at: 2427 2601 2840 2928 3497 3810 4683 4768 5839 Size: 174 239 88 569 313 873 85 1071 Cuts at: 5839 2427 Size: 3210 Fragments arranged by size: 3210 1071 873 569 313 239 174 88 85 BsmI GAATG_Cn' Cuts at: 4133 4226 4133 Size: 93 6529 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2427 2508 2535 2601 2778 2840 Size: 399 255 382 81 27 66 177 62 Cuts at: 2840 2928 3497 3810 3941 4683 4768 5839 1391 Size: 88 569 313 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 399 382 313 255 177 131 88 85 81 66 62 27 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 3240 4511 6241 3240 Size: 1271 1730 3621 Fragments arranged by size: 3621 1730 1271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3192 3325 3384 3977 844 Size: 2348 133 59 593 3489 Fragments arranged by size: 3489 2348 593 133 59 BsrBI CCG'CTC Cuts at: 1394 1823 3583 4509 1394 Size: 429 1760 926 3507 Fragments arranged by size: 3507 1760 926 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5117 5291 65 Size: 1183 3869 174 1396 Fragments arranged by size: 3869 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2681 2959 3497 5266 5453 1700 Size: 110 871 278 538 1769 187 2869 Fragments arranged by size: 2869 1769 871 538 278 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6622 BssHII G'CGCG_C Cuts at: 1925 2785 2818 2908 1925 Size: 860 33 90 5639 Fragments arranged by size: 5639 860 90 33 BssSI C'ACGA_G Cuts at: 4682 5694 4682 Size: 1012 5610 BstAPI GCAn_nnn'nTGC Cuts at: 794 2811 3184 794 Size: 2017 373 4232 Fragments arranged by size: 4232 2017 373 BstBI TT'CG_AA Cuts at: 5426 5426 Size: 6622 BstEII G'GTnAC_C Cuts at: 1184 3439 1184 Size: 2255 4367 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2131 Size: 6622 BstYI r'GATC_y Cuts at: 1972 3003 3048 3720 3952 4319 4704 4721 6173 Size: 1031 45 672 232 367 385 17 1452 Cuts at: 6173 6259 6271 6617 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1031 672 385 367 346 232 86 45 17 12 BstZ17I GTA'TAC Cuts at: 3340 3340 Size: 6622 BtgI C'CryG_G Cuts at: 513 1065 2014 2370 2535 2676 3132 513 Size: 552 949 356 165 141 456 4003 Fragments arranged by size: 4003 949 552 456 356 165 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2077 2533 487 Size: 683 56 851 456 4576 Fragments arranged by size: 4576 851 683 456 56 BtsI GCAGTG_nn' Cuts at: 4138 4932 4952 4138 Size: 794 20 5808 Fragments arranged by size: 5808 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6587 DraI TTT'AAA Cuts at: 3926 3926 Size: 6622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3208 5629 809 Size: 507 1892 2421 1802 Fragments arranged by size: 2421 1892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2276 2544 Size: 54 1425 50 275 18 354 92 268 Cuts at: 2544 2679 3144 3624 4069 4958 6587 8 Size: 135 465 480 445 889 1629 43 Fragments arranged by size: 1629 1425 889 480 465 445 354 275 268 135 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2679 4069 1537 Size: 275 867 1390 4090 Fragments arranged by size: 4090 1390 867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4552 1353 Size: 3199 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2753 3593 5209 5583 5729 387 Size: 1064 138 1164 840 1616 374 146 1280 Fragments arranged by size: 1616 1280 1164 1064 840 374 146 138 EcoICRI GAG'CTC Cuts at: 2838 2926 3808 2838 Size: 88 882 5652 Fragments arranged by size: 5652 882 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2975 3659 4685 5282 6069 6337 1239 Size: 393 1343 684 1026 597 787 268 1524 Fragments arranged by size: 1524 1343 1026 787 684 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2738 2738 Size: 6622 EcoO109I rG'GnC_Cy Cuts at: 2949 3181 3424 3899 2949 Size: 232 243 475 5672 Fragments arranged by size: 5672 475 243 232 EcoRI G'AATT_C Cuts at: 3931 4309 3931 Size: 378 6244 EcoRV GAT'ATC Cuts at: 2364 2364 Size: 6622 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 6622 FspI TGC'GCA Cuts at: 2519 2804 5128 2519 Size: 285 2324 4013 Fragments arranged by size: 4013 2324 285 HaeII r_GCGC'y Cuts at: 1678 2155 2473 2975 3132 5769 1678 Size: 477 318 502 157 2637 2531 Fragments arranged by size: 2637 2531 502 477 318 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 2657 2689 3891 Size: 32 135 32 776 32 277 32 1202 Cuts at: 3891 3923 5263 5295 5337 5369 1373 Size: 32 1340 32 42 32 2626 Fragments arranged by size: 2626 1340 1202 776 277 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3966 4212 669 Size: 988 281 2028 246 3079 Fragments arranged by size: 3079 2028 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6622 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KasI G'GCGC_C Cuts at: 3128 3128 Size: 6622 KpnI G_GTAC'C Cuts at: 3947 3947 Size: 6622 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2095 2241 5736 5920 1305 Size: 790 146 3495 184 2007 Fragments arranged by size: 3495 2007 790 184 146 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 10 Size: 54 1425 343 354 4446 Fragments arranged by size: 4446 1425 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 4580 4939 5098 518 Size: 648 1218 2196 359 159 2042 Fragments arranged by size: 2196 2042 1218 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3134 3230 3870 4038 4715 5863 6108 1606 Size: 1528 96 640 168 677 1148 245 2120 Fragments arranged by size: 2120 1528 1148 677 640 245 168 96 NaeI GCC'GGC Cuts at: 2683 2683 Size: 6622 NarI GG'CG_CC Cuts at: 3129 3129 Size: 6622 NcoI C'CATG_G Cuts at: 513 1065 2370 513 Size: 552 1305 4765 Fragments arranged by size: 4765 1305 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NgoMIV G'CCGG_C Cuts at: 2681 2681 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NruI TCG'CGA Cuts at: 3612 3612 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 3980 5525 1132 Size: 97 2751 1545 2229 Fragments arranged by size: 2751 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6622 PciI A'CATG_T Cuts at: 5521 5521 Size: 6622 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6622 PfoI T'CCnGG_A Cuts at: 3006 3006 Size: 6622 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2949 3181 3424 3899 2949 Size: 232 243 475 5672 Fragments arranged by size: 5672 475 243 232 PshAI GACnn'nnGTC Cuts at: 1613 3534 3732 1613 Size: 1921 198 4503 Fragments arranged by size: 4503 1921 198 PsiI TTA'TAA Cuts at: 4192 4192 Size: 6622 PspOMI G'GGCC_C Cuts at: 2774 2774 Size: 6622 PstI C_TGCA'G Cuts at: 830 3066 3974 830 Size: 2236 908 3478 Fragments arranged by size: 3478 2236 908 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 PvuII CAG'CTG Cuts at: 3230 3230 Size: 6622 SacI G_AGCT'C Cuts at: 2840 2928 3810 2840 Size: 88 882 5652 Fragments arranged by size: 5652 882 88 SacII CC_GC'GG Cuts at: 3135 3135 Size: 6622 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SanDI GG'GwC_CC Cuts at: 2949 3424 3899 2949 Size: 475 475 5672 Fragments arranged by size: 5672 475 475 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 4870 6582 4870 Size: 1712 4910 SexAI A'CCwGG_T Cuts at: 2178 2178 Size: 6622 SfcI C'TryA_G Cuts at: 826 1045 3062 3970 5105 5786 5977 826 Size: 219 2017 908 1135 681 191 1471 Fragments arranged by size: 2017 1471 1135 908 681 219 191 SfoI GGC'GCC Cuts at: 3130 3130 Size: 6622 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 3949 1872 Size: 2077 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4726 5627 5889 820 Size: 197 926 2783 901 262 1553 Fragments arranged by size: 2783 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3980 1132 Size: 2848 3774 SspI AAT'ATT Cuts at: 5 52 4546 5 Size: 47 4494 2081 Fragments arranged by size: 4494 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6622 StyI C'CwwG_G Cuts at: 513 1065 2370 2481 3363 3847 4061 513 Size: 552 1305 111 882 484 214 3074 Fragments arranged by size: 3074 1305 882 552 484 214 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2705 3630 3774 4649 4666 4819 5004 Size: 1255 1125 925 144 875 17 153 185 Cuts at: 5004 5449 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1125 925 875 445 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 4868 6580 Size: 274 80 33 51 1443 348 2543 1712 Cuts at: 6580 96 Size: 138 Fragments arranged by size: 2543 1712 1443 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6582 6614 6582 Size: 32 6590 Tth111I GACn'n_nGTC Cuts at: 1316 3617 1316 Size: 2301 4321 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 3634 3854 3913 2179 Size: 1455 220 59 4888 Fragments arranged by size: 4888 1455 220 59 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6622 XmaI C'CCGG_G Cuts at: 1870 3947 1870 Size: 2077 4545 XmnI GAAnn'nnTTC Cuts at: 4751 4751 Size: 6622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI Bsu36I DraIII FseI FspAI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI