Restriction map for
pFN21AB3769
(Linear) (Six-base) MAPSORT of: KIBB3769.seq Check: 9752 from: 1 to: 1917
KIBB3769.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 1334 1917
Size: 1334 583
AclI AA'CG_TT
Cuts at: 0 1469 1917
Size: 1469 448
AflIII A'CryG_T
Cuts at: 0 626 1917
Size: 626 1291
AgeI A'CCGG_T
Cuts at: 0 1492 1917
Size: 1492 425
AlwNI CAG_nnn'CTG
Cuts at: 0 1053 1179 1917
Size: 1053 126 738
Fragments arranged by size:
1053 738 126
ApaI G_GGCC'C
Cuts at: 0 773 1917
Size: 773 1144
ApaLI G'TGCA_C
Cuts at: 0 418 1917
Size: 418 1499
ApoI r'AATT_y
Cuts at: 0 232 1917
Size: 232 1685
BanI G'GyrC_C
Cuts at: 0 37 487 1123 1917
Size: 37 450 636 794
Fragments arranged by size:
794 636 450 37
BanII G_rGCy'C
Cuts at: 0 503 530 773 835 923 1805 1917
Size: 503 27 243 62 88 882 112
Fragments arranged by size:
882 503 243 112 88 62 27
BbeI G_GCGC'C
Cuts at: 0 1127 1917
Size: 1127 790
BbsI GAAGACnn'nnnn_
Cuts at: 0 54 1676 1917
Size: 54 1622 241
Fragments arranged by size:
1622 241 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 840 874 1917
Size: 840 34 1043
Fragments arranged by size:
1043 840 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1045 1917
Size: 1045 872
BclI T'GATC_A
Cuts at: 0 1739 1917
Size: 1739 178
BglI GCCn_nnn'nGGC
Cuts at: 0 155 1917
Size: 155 1762
Bme1580I G_kGCm'C
Cuts at: 0 40 422 773 1917
Size: 40 382 351 1144
Fragments arranged by size:
1144 382 351 40
BmgBI CAC'GTC
Cuts at: 0 627 1194 1917
Size: 627 567 723
Fragments arranged by size:
723 627 567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 879 1917
Size: 378 501 1038
Fragments arranged by size:
1038 501 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1654 1917
Size: 970 684 263
Fragments arranged by size:
970 684 263
Bpu10I CC'TnA_GC
Cuts at: 0 829 1917
Size: 829 1088
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 1917
Size: 325 1592
BsaAI yAC'GTr
Cuts at: 0 126 1917
Size: 126 1791
BsaHI Gr'CG_yC
Cuts at: 0 161 209 977 1124 1451 1917
Size: 161 48 768 147 327 466
Fragments arranged by size:
768 466 327 161 147 48
BsaWI w'CCGG_w
Cuts at: 0 1492 1556 1917
Size: 1492 64 361
Fragments arranged by size:
1492 361 64
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 751 931 1917
Size: 367 384 180 986
Fragments arranged by size:
986 384 367 180
BseYI C'CCAG_C
Cuts at: 0 118 1209 1374 1917
Size: 118 1091 165 543
Fragments arranged by size:
1091 543 165 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1153 1192 1348 1585 1917
Size: 271 882 39 156 237 332
Fragments arranged by size:
882 332 271 237 156 39
BsiEI CG_ry'CG
Cuts at: 0 677 1611 1755 1917
Size: 677 934 144 162
Fragments arranged by size:
934 677 162 144
BsiHKAI G_wGCw'C
Cuts at: 0 422 596 835 923 1492 1805 1917
Size: 422 174 239 88 569 313 112
Fragments arranged by size:
569 422 313 239 174 112 88
Bsp1286I G_dGCh'C
Cuts at: 0 40 422 503 530 596 773 835 923
Size: 40 382 81 27 66 177 62 88
Cuts at: 923 1492 1805 1917
Size: 569 313 112
Fragments arranged by size:
569 382 313 177 112 88 81 66
62 40 27
BspHI T'CATG_A
Cuts at: 0 1235 1917
Size: 1235 682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1187 1320 1379 1917
Size: 1187 133 59 538
Fragments arranged by size:
1187 538 133 59
BsrBI CCG'CTC
Cuts at: 0 1578 1917
Size: 1578 339
BsrFI r'CCGG_y
Cuts at: 0 676 954 1492 1917
Size: 676 278 538 425
Fragments arranged by size:
676 538 425 278
BssHII G'CGCG_C
Cuts at: 0 780 813 903 1917
Size: 780 33 90 1014
Fragments arranged by size:
1014 780 90 33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 806 1179 1917
Size: 806 373 738
Fragments arranged by size:
806 738 373
BstEII G'GTnAC_C
Cuts at: 0 1434 1917
Size: 1434 483
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 1917
Size: 126 1791
BstYI r'GATC_y
Cuts at: 0 998 1043 1715 1917
Size: 998 45 672 202
Fragments arranged by size:
998 672 202 45
BstZ17I GTA'TAC
Cuts at: 0 1335 1917
Size: 1335 582
BtgI C'CryG_G
Cuts at: 0 9 365 530 671 1127 1917
Size: 9 356 165 141 456 790
Fragments arranged by size:
790 456 356 165 141 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 72 528 1917
Size: 72 456 1389
Fragments arranged by size:
1389 456 72
DrdI GACnn_nn'nnGTC
Cuts at: 0 1203 1917
Size: 1203 714
EaeI y'GGCC_r
Cuts at: 0 179 271 539 674 1139 1619 1917
Size: 179 92 268 135 465 480 298
Fragments arranged by size:
480 465 298 268 179 135 92
EagI C'GGCC_G
Cuts at: 0 674 1917
Size: 674 1243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 748 1588 1917
Size: 748 840 329
Fragments arranged by size:
840 748 329
EcoICRI GAG'CTC
Cuts at: 0 833 921 1803 1917
Size: 833 88 882 114
Fragments arranged by size:
882 833 114 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1654 1917
Size: 970 684 263
Fragments arranged by size:
970 684 263
EcoNI CCTnn'n_nnAGG
Cuts at: 0 733 1917
Size: 733 1184
EcoO109I rG'GnC_Cy
Cuts at: 0 944 1176 1419 1894 1917
Size: 944 232 243 475 23
Fragments arranged by size:
944 475 243 232 23
EcoRV GAT'ATC
Cuts at: 0 359 1917
Size: 359 1558
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 360 1917
Size: 360 1557
FspI TGC'GCA
Cuts at: 0 514 799 1917
Size: 514 285 1118
Fragments arranged by size:
1118 514 285
HaeII r_GCGC'y
Cuts at: 0 150 468 970 1127 1917
Size: 150 318 502 157 790
Fragments arranged by size:
790 502 318 157 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 652 684 1886 1917
Size: 343 32 277 32 1202 31
Fragments arranged by size:
1202 343 277 32 32 31
KasI G'GCGC_C
Cuts at: 0 1123 1917
Size: 1123 794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 236 1917
Size: 90 146 1681
Fragments arranged by size:
1681 146 90
MscI TGG'CCA
Cuts at: 0 181 1917
Size: 181 1736
MslI CAynn'nnrTG
Cuts at: 0 379 1917
Size: 379 1538
MspA1I CmG'CkG
Cuts at: 0 1129 1225 1865 1917
Size: 1129 96 640 52
Fragments arranged by size:
1129 640 96 52
NaeI GCC'GGC
Cuts at: 0 678 1917
Size: 678 1239
NarI GG'CG_CC
Cuts at: 0 1124 1917
Size: 1124 793
NcoI C'CATG_G
Cuts at: 0 365 1917
Size: 365 1552
NgoMIV G'CCGG_C
Cuts at: 0 676 1917
Size: 676 1241
NruI TCG'CGA
Cuts at: 0 1607 1917
Size: 1607 310
PfoI T'CCnGG_A
Cuts at: 0 1001 1917
Size: 1001 916
PpuMI rG'GwC_Cy
Cuts at: 0 944 1176 1419 1894 1917
Size: 944 232 243 475 23
Fragments arranged by size:
944 475 243 232 23
PshAI GACnn'nnGTC
Cuts at: 0 1529 1727 1917
Size: 1529 198 190
Fragments arranged by size:
1529 198 190
PspOMI G'GGCC_C
Cuts at: 0 769 1917
Size: 769 1148
PstI C_TGCA'G
Cuts at: 0 1061 1917
Size: 1061 856
PvuII CAG'CTG
Cuts at: 0 1225 1917
Size: 1225 692
SacI G_AGCT'C
Cuts at: 0 835 923 1805 1917
Size: 835 88 882 112
Fragments arranged by size:
882 835 112 88
SacII CC_GC'GG
Cuts at: 0 1130 1917
Size: 1130 787
SanDI GG'GwC_CC
Cuts at: 0 944 1419 1894 1917
Size: 944 475 475 23
Fragments arranged by size:
944 475 475 23
SexAI A'CCwGG_T
Cuts at: 0 173 1917
Size: 173 1744
SfcI C'TryA_G
Cuts at: 0 1057 1917
Size: 1057 860
SfoI GGC'GCC
Cuts at: 0 1125 1917
Size: 1125 792
StyI C'CwwG_G
Cuts at: 0 365 476 1358 1842 1917
Size: 365 111 882 484 75
Fragments arranged by size:
882 484 365 111 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 1625 1769 1917
Size: 700 925 144 148
Fragments arranged by size:
925 700 148 144
TatI w'GTAC_w
Cuts at: 0 320 1917
Size: 320 1597
Tth111I GACn'n_nGTC
Cuts at: 0 1612 1917
Size: 1612 305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 1629 1849 1908 1917
Size: 174 1455 220 59 9
Fragments arranged by size:
1455 220 174 59 9
Enzymes that do cut:
AccI AclI AflIII AgeI AlwNI ApaI ApaLI ApoI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstYI
BstZ17I BtgI BtgZI DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SanDI SexAI SfcI SfoI StyI TaqII
TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AhdI AleI
AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbvCI BfrBI BglII BlpI BmrI BmtI BpuEI
BsaBI BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI
BssSI BstBI Bsu36I BtsI ClaI CspCI DraI DraIII
EarI EcoRI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XhoI XmaI XmnI
ZraI