################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KpnI Ksp632I MaeI MflI MluI MroI MseI MslI NarI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Alw44I 1044 GTGCAC G^TGCAC AlwI 562 GGATC GGATC(4/5) AlwNI 52 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 995 AGCGCT AGC^GCT ApaLI 1044 GTGCAC G^TGCAC AspI 829 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 866 TGCGCA TGC^GCA BalI 168 TGGCCA TGG^CCA BanI 127 GGTGCC G^G[CT][GA]CC BcgI 1046 GCACGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 398 GCCGCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 49 GCTCAGC GC^T[ACGT]AGC BpmI 787 CTGGAG CTGGAG(16/14) Bpu1102I 49 GCTCAGC GC^T[ACGT]AGC BsaBI 114 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 568 GAGGAG GAGGAG(10/8) BsmBI 663 CGTCTC CGTCTC(1/5) BsmI 244 GAATGC GAATGC(1/-1) BspHI 587 TCATGA T^CATGA CelII 49 GCTCAGC GC^T[ACGT]AGC DdeI 50 CTCAG C^T[ACGT]AG DraIII 352 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 755 CGGCCG C^GGCCG EclXI 755 CGGCCG C^GGCCG Eco47III 995 AGCGCT AGC^GCT Eco52I 755 CGGCCG C^GGCCG FspI 866 TGCGCA TGC^GCA HinfI 314 GAGTC G^A[ACGT]TC KspI 365 CCGCGG CCGC^GG MaeIII 769 GTCAC ^GT[ACGT]AC MamI 114 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 546 GAAGA GAAGA(8/7) MfeI 187 CAATTG C^AATTG MluNI 168 TGGCCA TGG^CCA MscI 168 TGGCCA TGG^CCA MunI 187 CAATTG C^AATTG NaeI 782 GCCGGC GCC^GGC NgoMIV 782 GCCGGC G^CCGGC NotI 754 GCGGCCGC GC^GGCCGC PflFI 829 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 68 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 314 GAGTC GAGTC(4/5) RcaI 587 TCATGA T^CATGA SacII 365 CCGCGG CCGC^GG SexAI 830 ACCTGGT A^CC[AT]GGT SmlI 682 CTCAAG C^T[CT][GA]AG Tsp45I 769 GTCAC ^GT[GC]AC Tth111I 829 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 68 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 515 CCAAGGAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 64 GACGCC G[GA]^CG[CT]C 661 GGCGTC BsaHI 64 GACGCC G[GA]^CG[CT]C 661 GGCGTC BsaI 105 GAGACC GGTCTC(1/5) 337 GAGACC BsiEI 612 CGGTCG CG[GA][CT]^CG 755 CGGCCG BstF5I 528 GGATG GGATG(2/0) 933 GGATG DpnII 563 GATC ^GATC 1053 GATC DsaI 365 CCGCGG C^C[GA][CT]GG 657 CCACGG EcoT14I 446 CCAAGG C^C[AT][AT]GG 515 CCAAGG FokI 528 GGATG GGATG(9/13) 933 GGATG HaeII 361 AGCGCC [GA]GCGC^[CT] 995 AGCGCT Hin1I 64 GACGCC G[GA]^CG[CT]C 661 GGCGTC HincII 376 GTCAAC GT[CT]^[GA]AC 796 GTCAAC HindII 376 GTCAAC GT[CT]^[GA]AC 796 GTCAAC MaeII 491 ACGT A^CGT 641 ACGT MboI 563 GATC ^GATC 1053 GATC NdeII 563 GATC ^GATC 1053 GATC PvuII 42 CAGCTG CAG^CTG 700 CAGCTG SacI 409 GAGCTC GAGCT^C 943 GAGCTC Sau3AI 563 GATC ^GATC 1053 GATC StyI 446 CCAAGG C^C[AT][AT]GG 515 CCAAGG Tsp509I 188 AATT ^AATT 277 AATT TspRI 1003 CACTG CA[GC]TG[ACGT][ACGT]^ 1015 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PpuMI PstI RsaI Sau96I ScrFI SfaNI SfcI SmaI TseI XmaCI XmaI