(Circular) (Six-base) MAPSORT of: pFN21AE1451.seq Check: 9999 from: 1 to: 5782 pFN21AE1451 5782 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2432 2983 2432 Size: 551 5231 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2759 3845 5229 5497 1632 Size: 1127 1086 1384 268 1917 Fragments arranged by size: 1917 1384 1127 1086 268 AfeI AGC'GCT Cuts at: 3002 3002 Size: 5782 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2062 3525 5097 2062 Size: 1463 1572 2747 Fragments arranged by size: 2747 1572 1463 ApaLI G'TGCA_C Cuts at: 3049 3839 4995 3049 Size: 790 1156 3836 Fragments arranged by size: 3836 1156 790 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2159 2463 2538 2671 3107 1870 Size: 73 171 45 304 75 133 436 4545 Fragments arranged by size: 4545 436 304 171 133 75 73 45 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 3103 4558 618 Size: 325 217 627 345 971 1455 1842 Fragments arranged by size: 1842 1455 971 627 345 325 217 BanII G_rGCy'C Cuts at: 1391 2056 2166 2418 2891 2952 3101 1391 Size: 665 110 252 473 61 149 4072 Fragments arranged by size: 4072 665 473 252 149 110 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3040 3074 3973 4007 1903 Size: 34 50 34 1019 34 899 34 3678 Fragments arranged by size: 3678 1019 899 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2409 4393 136 Size: 107 122 71 631 605 737 1984 1525 Fragments arranged by size: 1984 1525 737 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 2055 3199 2055 Size: 1144 4638 Bme1580I G_kGCm'C Cuts at: 1790 2137 2900 3053 3843 4999 1790 Size: 347 763 153 790 1156 2573 Fragments arranged by size: 2573 1156 790 763 347 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2813 4442 1239 Size: 1574 1629 2579 Fragments arranged by size: 2579 1629 1574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 3907 4772 5070 2672 Size: 1235 865 298 3384 Fragments arranged by size: 3384 1235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2104 2336 4445 882 Size: 617 605 232 2109 2219 Fragments arranged by size: 2219 2109 617 605 232 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 2123 5776 2123 Size: 3653 2129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2667 3589 3971 Size: 53 83 186 936 537 597 922 382 Cuts at: 3971 4606 275 Size: 635 1451 Fragments arranged by size: 1451 936 922 635 597 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 1178 Size: 30 165 30 657 30 4870 Fragments arranged by size: 4870 657 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2588 1186 Size: 1402 4380 BseYI C'CCAG_C Cuts at: 2009 2049 2136 2817 2852 4985 5481 2009 Size: 40 87 681 35 2133 496 2310 Fragments arranged by size: 2310 2133 681 496 87 40 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 2167 3011 1163 Size: 573 390 41 844 3934 Fragments arranged by size: 3934 844 573 390 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2620 2763 3232 3993 5021 1540 Size: 275 186 619 143 469 761 1028 2301 Fragments arranged by size: 2301 1028 761 619 469 275 186 143 BsiHKAI G_wGCw'C Cuts at: 2208 2418 2592 2885 2952 3053 3843 3928 4999 Size: 210 174 293 67 101 790 85 1071 Cuts at: 4999 2208 Size: 2991 Fragments arranged by size: 2991 1071 790 293 210 174 101 85 67 BsmI GAATG_Cn' Cuts at: 2255 3293 3386 2255 Size: 1038 93 4651 Fragments arranged by size: 4651 1038 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2674 2674 Size: 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2137 2166 2208 2418 2592 2885 Size: 399 266 81 29 42 210 174 293 Cuts at: 2885 2891 2900 2952 3053 3101 3843 3928 4999 Size: 6 9 52 101 48 742 85 1071 Cuts at: 4999 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 293 266 210 174 101 85 81 52 48 42 29 9 6 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 2592 3671 5401 2592 Size: 1079 1730 2973 Fragments arranged by size: 2973 1730 1079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 2466 2607 2702 2983 3137 844 Size: 1588 34 141 95 281 154 3489 Fragments arranged by size: 3489 1588 281 154 141 95 34 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2615 2733 2787 4426 4613 1700 Size: 110 805 118 54 1639 187 2869 Fragments arranged by size: 2869 1639 805 187 118 110 54 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2370 2662 513 Size: 552 1305 292 3633 Fragments arranged by size: 3633 1305 552 292 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 3007 3298 4092 4112 3007 Size: 291 794 20 4677 Fragments arranged by size: 4677 794 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DraIII CAC_nnn'GTG Cuts at: 2362 2362 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2173 2709 2760 Size: 54 1425 50 275 18 343 536 51 Cuts at: 2760 3229 4118 5747 8 Size: 469 889 1629 43 Fragments arranged by size: 1629 1425 889 536 469 343 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2760 3229 1537 Size: 275 948 469 4090 Fragments arranged by size: 4090 948 469 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3712 1353 Size: 2359 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2416 2950 2416 Size: 534 5248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2759 2813 3845 4442 5229 5497 1239 Size: 393 1127 54 1032 597 787 268 1524 Fragments arranged by size: 1524 1127 1032 787 597 393 268 54 EcoO109I rG'GnC_Cy Cuts at: 2455 2676 2833 2455 Size: 221 157 5404 Fragments arranged by size: 5404 221 157 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 2873 4288 2873 Size: 1415 4367 HaeII r_GCGC'y Cuts at: 1678 2370 3004 4929 1678 Size: 692 634 1925 2531 Fragments arranged by size: 2531 1925 692 634 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2288 2320 4423 Size: 32 135 32 488 32 196 32 2103 Cuts at: 4423 4455 4497 4529 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2103 488 196 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 2803 3126 3372 669 Size: 988 281 445 420 323 246 3079 Fragments arranged by size: 3079 988 445 420 323 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 2192 3381 2192 Size: 1189 4593 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2175 10 Size: 54 1425 343 343 3617 Fragments arranged by size: 3617 1425 343 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4099 4258 518 Size: 648 2574 359 159 2042 Fragments arranged by size: 2574 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2272 2294 2372 2707 3198 3875 5023 Size: 443 223 22 78 335 491 677 1148 Cuts at: 5023 5268 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 491 443 335 245 223 78 22 NaeI GCC'GGC Cuts at: 2789 2789 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NgoMIV G'CCGG_C Cuts at: 2787 2787 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 2760 3229 2760 Size: 469 5313 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 2893 1348 Size: 1545 4237 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 2079 1108 Size: 971 4811 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2455 2676 2833 2455 Size: 221 157 5404 Fragments arranged by size: 5404 221 157 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2435 2684 2706 3134 830 Size: 1605 249 22 428 3478 Fragments arranged by size: 3478 1605 428 249 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2049 2707 2049 Size: 658 5124 SacI G_AGCT'C Cuts at: 2418 2952 2418 Size: 534 5248 SacII CC_GC'GG Cuts at: 2373 2373 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SexAI A'CCwGG_T Cuts at: 2835 2835 Size: 5782 SfcI C'TryA_G Cuts at: 826 1045 2431 2680 2702 3130 4265 4946 5137 Size: 219 1386 249 22 428 1135 681 191 Cuts at: 5137 826 Size: 1471 Fragments arranged by size: 1471 1386 1135 681 428 249 219 191 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2161 2465 2540 3109 1872 Size: 289 304 75 569 4545 Fragments arranged by size: 4545 569 304 289 75 SmlI C'TyrA_G Cuts at: 820 1017 1943 2687 3886 4787 5049 820 Size: 197 926 744 1199 901 262 1553 Fragments arranged by size: 1553 1199 926 901 744 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2451 2520 3221 513 Size: 552 1386 69 701 3074 Fragments arranged by size: 3074 1386 701 552 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 2831 3809 3826 3979 4164 4609 Size: 1255 789 462 978 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1498 1255 978 789 462 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3069 4028 5740 Size: 274 80 33 51 1443 1092 959 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1712 1443 1092 959 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2459 2491 5742 5774 2459 Size: 32 3251 32 2467 Fragments arranged by size: 3251 2467 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2837 1316 Size: 1521 4261 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2527 2527 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 2159 2463 2538 3107 1870 Size: 289 304 75 569 4545 Fragments arranged by size: 4545 569 304 289 75 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI