(Linear) (Six-base) MAPSORT of: KIEE1451.seq Check: 1393 from: 1 to: 1077 KIEE1451.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 427 978 1077 Size: 427 551 99 Fragments arranged by size: 551 427 99 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 754 1077 Size: 754 323 AfeI AGC'GCT Cuts at: 0 997 1077 Size: 997 80 AlwNI CAG_nnn'CTG Cuts at: 0 57 1077 Size: 57 1020 ApaLI G'TGCA_C Cuts at: 0 1044 1077 Size: 1044 33 AvaI C'yCGr_G Cuts at: 0 109 154 458 533 666 1077 Size: 109 45 304 75 133 411 Fragments arranged by size: 411 304 133 109 75 45 BanI G'GyrC_C Cuts at: 0 127 1077 Size: 127 950 BanII G_rGCy'C Cuts at: 0 51 161 413 886 947 1077 Size: 51 110 252 473 61 130 Fragments arranged by size: 473 252 130 110 61 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1035 1069 1077 Size: 1035 34 8 Fragments arranged by size: 1035 34 8 BglI GCCn_nnn'nGGC Cuts at: 0 404 1077 Size: 404 673 BlpI GC'TnA_GC Cuts at: 0 50 1077 Size: 50 1027 Bme1580I G_kGCm'C Cuts at: 0 132 895 1048 1077 Size: 132 763 153 29 Fragments arranged by size: 763 153 132 29 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 1077 Size: 808 269 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 1077 Size: 667 410 BsaI GGTCTCn'nnnn_ Cuts at: 0 99 331 1077 Size: 99 232 746 Fragments arranged by size: 746 232 99 BsaBI GATnn'nnATC Cuts at: 0 118 1077 Size: 118 959 BsaHI Gr'CG_yC Cuts at: 0 65 662 1077 Size: 65 597 415 Fragments arranged by size: 597 415 65 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 55 85 1077 Size: 55 30 992 Fragments arranged by size: 992 55 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 583 1077 Size: 583 494 BseYI C'CCAG_C Cuts at: 0 4 44 131 812 847 1077 Size: 4 40 87 681 35 230 Fragments arranged by size: 681 230 87 40 35 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 162 1006 1077 Size: 121 41 844 71 Fragments arranged by size: 844 121 71 41 BsiEI CG_ry'CG Cuts at: 0 615 758 1077 Size: 615 143 319 Fragments arranged by size: 615 319 143 BsiHKAI G_wGCw'C Cuts at: 0 203 413 587 880 947 1048 1077 Size: 203 210 174 293 67 101 29 Fragments arranged by size: 293 210 203 174 101 67 29 BsmI GAATG_Cn' Cuts at: 0 250 1077 Size: 250 827 BsmBI CGTCTCn'nnnn_ Cuts at: 0 669 1077 Size: 669 408 Bsp1286I G_dGCh'C Cuts at: 0 51 132 161 203 413 587 880 886 Size: 51 81 29 42 210 174 293 6 Cuts at: 886 895 947 1048 1077 Size: 9 52 101 29 Fragments arranged by size: 293 210 174 101 81 52 51 42 29 29 9 6 BspHI T'CATG_A Cuts at: 0 587 1077 Size: 587 490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 427 461 602 697 978 1077 Size: 427 34 141 95 281 99 Fragments arranged by size: 427 281 141 99 95 34 BsrFI r'CCGG_y Cuts at: 0 610 728 782 1077 Size: 610 118 54 295 Fragments arranged by size: 610 295 118 54 BtgI C'CryG_G Cuts at: 0 365 657 1077 Size: 365 292 420 Fragments arranged by size: 420 365 292 BtsI GCAGTG_nn' Cuts at: 0 1002 1077 Size: 1002 75 DraIII CAC_nnn'GTG Cuts at: 0 357 1077 Size: 357 720 EaeI y'GGCC_r Cuts at: 0 168 704 755 1077 Size: 168 536 51 322 Fragments arranged by size: 536 322 168 51 EagI C'GGCC_G Cuts at: 0 755 1077 Size: 755 322 EcoICRI GAG'CTC Cuts at: 0 411 945 1077 Size: 411 534 132 Fragments arranged by size: 534 411 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 754 808 1077 Size: 754 54 269 Fragments arranged by size: 754 269 54 EcoO109I rG'GnC_Cy Cuts at: 0 450 671 828 1077 Size: 450 221 157 249 Fragments arranged by size: 450 249 221 157 FspI TGC'GCA Cuts at: 0 868 1077 Size: 868 209 HaeII r_GCGC'y Cuts at: 0 365 999 1077 Size: 365 634 78 Fragments arranged by size: 634 365 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 55 87 283 315 1077 Size: 55 32 196 32 762 Fragments arranged by size: 762 196 55 32 32 HincII GTy'rAC Cuts at: 0 378 798 1077 Size: 378 420 279 Fragments arranged by size: 420 378 279 MfeI C'AATT_G Cuts at: 0 187 1077 Size: 187 890 MscI TGG'CCA Cuts at: 0 170 1077 Size: 170 907 MspA1I CmG'CkG Cuts at: 0 44 267 289 367 702 1077 Size: 44 223 22 78 335 375 Fragments arranged by size: 375 335 223 78 44 22 NaeI GCC'GGC Cuts at: 0 784 1077 Size: 784 293 NgoMIV G'CCGG_C Cuts at: 0 782 1077 Size: 782 295 NotI GC'GGCC_GC Cuts at: 0 755 1077 Size: 755 322 PasI CC'CwG_GG Cuts at: 0 888 1077 Size: 888 189 PflMI CCAn_nnn'nTGG Cuts at: 0 74 1077 Size: 74 1003 PpuMI rG'GwC_Cy Cuts at: 0 450 671 828 1077 Size: 450 221 157 249 Fragments arranged by size: 450 249 221 157 PstI C_TGCA'G Cuts at: 0 430 679 701 1077 Size: 430 249 22 376 Fragments arranged by size: 430 376 249 22 PvuII CAG'CTG Cuts at: 0 44 702 1077 Size: 44 658 375 Fragments arranged by size: 658 375 44 SacI G_AGCT'C Cuts at: 0 413 947 1077 Size: 413 534 130 Fragments arranged by size: 534 413 130 SacII CC_GC'GG Cuts at: 0 368 1077 Size: 368 709 SexAI A'CCwGG_T Cuts at: 0 830 1077 Size: 830 247 SfcI C'TryA_G Cuts at: 0 426 675 697 1077 Size: 426 249 22 380 Fragments arranged by size: 426 380 249 22 SmaI CCC'GGG Cuts at: 0 156 460 535 1077 Size: 156 304 75 542 Fragments arranged by size: 542 304 156 75 SmlI C'TyrA_G Cuts at: 0 682 1077 Size: 682 395 StyI C'CwwG_G Cuts at: 0 446 515 1077 Size: 446 69 562 Fragments arranged by size: 562 446 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 364 826 1077 Size: 364 462 251 Fragments arranged by size: 462 364 251 TatI w'GTAC_w Cuts at: 0 1064 1077 Size: 1064 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 454 486 1077 Size: 454 32 591 Fragments arranged by size: 591 454 32 Tth111I GACn'n_nGTC Cuts at: 0 832 1077 Size: 832 245 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 522 1077 Size: 522 555 XmaI C'CCGG_G Cuts at: 0 154 458 533 1077 Size: 154 304 75 544 Fragments arranged by size: 544 304 154 75 Enzymes that do cut: AarI AcuI AfeI AlwNI ApaLI AvaI BanI BanII BcgI BglI BlpI Bme1580I BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII MfeI MscI MspA1I NaeI NgoMIV NotI PasI PflMI PpuMI PstI PvuII SacI SacII SexAI SfcI SmaI SmlI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaWI BsiWI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MluI MmeI MslI NarI NcoI NdeI NheI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI