Restriction map for
pFN21AB6876
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DdeI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI Ksp632I MaeI
MamI MfeI MluI MroI MseI
MslI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp45I Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 92 GGTACC G^GTACC
AccII 282 CGCG CG^CG
AlwNI 298 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 20 GGGCCC GGGCC^C
Asp700I 507 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 92 GGTACC G^GTACC
AspHI 84 GTGCTC G[AT]GC[AT]^C
AvaI 403 CCCGAG C^[CT]CG[GA]G
AvaII 344 GGACC G^G[AT]CC
BalI 530 TGGCCA TGG^CCA
BanII 20 GGGCCC G[GA]GC[CT]^C
BclI 395 TGATCA T^GATCA
BglII 461 AGATCT A^GATCT
BsgI 268 CTGCAC GTGCAG(16/14)
BsiEI 123 CGGCCG CG[GA][CT]^CG
BsiHKAI 84 GTGCTC G[AT]GC[AT]^C
BsoBI 403 CCCGAG C^[CT]CG[GA]G
BsrFI 121 ACCGGC [GA]^CCGG[CT]
BstUI 282 CGCG CG^CG
Cfr10I 121 ACCGGC [GA]^CCGG[CT]
DsaI 281 CCGCGG C^C[GA][CT]GG
EagI 123 CGGCCG C^GGCCG
EclXI 123 CGGCCG C^GGCCG
Eco52I 123 CGGCCG C^GGCCG
EcoNI 24 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 511 GAATTC G^AATTC
FauI 283 GCGGG CCCGC(4/6)
FbaI 395 TGATCA T^GATCA
HinfI 220 GATTC G^A[ACGT]TC
HphI 131 TCACC GGTGA(8/7)
KpnI 92 GGTACC GGTAC^C
KspI 281 CCGCGG CCGC^GG
MaeIII 338 GTAAC ^GT[ACGT]AC
MluNI 530 TGGCCA TGG^CCA
MscI 530 TGGCCA TGG^CCA
MvnI 282 CGCG CG^CG
MwoI 441 GCCGGAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 201 GCATGT [GA]CATG^[CT]
PflMI 297 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 49 CTGCAG CTGCA^G
PvuII 52 CAGCTG CAG^CTG
SacII 281 CCGCGG CCGC^GG
ScaI 470 AGTACT AGT^ACT
SfcI 49 CTGCAG C^T[GA][CT]AG
StuI 188 AGGCCT AGG^CCT
TfiI 220 GATTC G^A[AT]TC
TspRI 527 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 297 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 507 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 511 GAATTC [GA]^AATT[CT]
542 AAATTC
AlwI 36 GATCC GGATC(4/5)
555 GATCC
ApoI 511 GAATTC [GA]^AATT[CT]
542 AAATTC
BanI 92 GGTACC G^G[CT][GA]CC
235 GGCACC
BmyI 20 GGGCCC G[AGT]GC[ACT]^C
84 GTGCTC
BsiYI 24 CCTGTCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
297 CCAGAAGCTGG
BslI 24 CCTGTCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
297 CCAGAAGCTGG
Bsp1286I 20 GGGCCC G[AGT]GC[ACT]^C
84 GTGCTC
BstYI 35 AGATCC [GA]^GATC[CT]
461 AGATCT
Cac8I 283 GCGGGC GC[ACGT]^[ACGT]GC
450 GCGAGC
DraII 19 GGGGCCC [GA]G^G[ACGT]CC[CT]
20 GGGCCCT
EaeI 123 CGGCCG [CT]^GGCC[GA]
530 TGGCCA
EcoO109I 19 GGGGCCC [GA]G^G[ACGT]CC[CT]
20 GGGCCCT
EcoT14I 29 CCAAGG C^C[AT][AT]GG
185 CCAAGG
HapII 122 CCGG C^CGG
442 CCGG
HpaII 122 CCGG C^CGG
442 CCGG
MflI 35 AGATCC [GA]^GATC[CT]
461 AGATCT
MspA1I 52 CAGCTG C[AC]G^C[GT]G
281 CCGCGG
MspI 122 CCGG C^CGG
442 CCGG
SfaNI 128 GCATC GCATC(5/9)
152 GCATC
StyI 29 CCAAGG C^C[AT][AT]GG
185 CCAAGG
TaqI 218 TCGA T^CGA
230 TCGA
Tsp509I 512 AATT ^AATT
543 AATT
TthHB8I 218 TCGA T^CGA
230 TCGA
XcmI 29 CCAAGGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
293 CCAACCAGAAGCTGG
XhoII 35 AGATCC [GA]^GATC[CT]
461 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BbvI BpmI
BsaJI BseRI BsmFI BsrI BssKI
BstNI Cfr13I DpnII EcoRII Fnu4HI
HaeIII ItaI MaeII MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI