################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeII MamI MfeI MluI MluNI MscI MunI MvnI NarI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 451 GGTACC G^GTACC Asp718I 451 GGTACC G^GTACC AspI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 491 GGATCC G^GATCC BanI 451 GGTACC G^G[CT][GA]CC BanII 616 GAGCTC G[GA]GC[CT]^C BbsI 180 GAAGAC GAAGAC(2/6) BbvCI 756 GCTGAGG CCTCAGC(-5/-2) BcnI 771 CCGGG CC^[GC]GG BfaI 545 CTAG C^TAG BglI 835 GCCAATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 321 GCTCAGC GC^T[ACGT]AGC BpmI 389 CTGGAG CTGGAG(16/14) Bpu1102I 321 GCTCAGC GC^T[ACGT]AGC BpuAI 180 GAAGAC GAAGAC(2/6) BsgI 716 CTGCAC GTGCAG(16/14) BsmAI 702 GTCTC GTCTC(1/5) Bsp1407I 794 TGTACA T^GTACA BspMI 38 ACCTGC ACCTGC(4/8) BsrBI 28 CCGCTC CCGCTC(-3/-3) BsrDI 651 CATTGC GCAATG(2/0) BsrFI 46 GCCGGC [GA]^CCGG[CT] BsrGI 794 TGTACA T^GTACA BstEII 82 GGTCACC G^GT[ACGT]ACC BstPI 82 GGTCACC G^GT[ACGT]ACC BstXI 480 CCAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 321 GCTCAGC GC^T[ACGT]AGC Cfr10I 46 GCCGGC [GA]^CCGG[CT] DraII 160 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 160 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 82 GGTCACC G^GT[ACGT]ACC FauI 27 CCCGC CCCGC(4/6) KpnI 451 GGTACC GGTAC^C MaeI 545 CTAG C^TAG MseI 881 TTAA T^TAA MspA1I 344 CAGCTG C[AC]G^C[GT]G NaeI 46 GCCGGC GCC^GGC NciI 771 CCGGG CC^[GC]GG NdeI 566 CATATG CA^TATG NgoMIV 46 GCCGGC G^CCGGC PflFI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 541 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 160 GGGACCT [GA]G^G[AT]CC[CT] PstI 112 CTGCAG CTGCA^G PvuII 344 CAGCTG CAG^CTG SacI 616 GAGCTC GAGCT^C SfcI 112 CTGCAG C^T[GA][CT]AG SspBI 794 TGTACA T^GTACA TaqI 705 TCGA T^CGA Tru9I 881 TTAA T^TAA Tsp509I 883 AATT ^AATT Tth111I 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 705 TCGA T^CGA Van91I 541 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccIII 176 TCCGGA T^CCGGA 594 TCCGGA AvaII 105 GGTCC G^G[AT]CC 161 GGACC BbvI 343 GCAGC GCAGC(8/12) 226 GCTGC BsaWI 176 TCCGGA [AT]^CCGG[AT] 594 TCCGGA BseAI 176 TCCGGA T^CCGGA 594 TCCGGA BspEI 176 TCCGGA T^CCGGA 594 TCCGGA BspHI 380 TCATGA T^CATGA 560 TCATGA BsrI 448 ACTGG ACTGG(1/-1) 668 ACTGG EaeI 2 TGGCCG [CT]^GGCC[GA] 44 TGGCCG EcoT14I 455 CCTTGG C^C[AT][AT]GG 852 CCTTGG HphI 84 TCACC GGTGA(8/7) 368 TCACC MroI 176 TCCGGA T^CCGGA 594 TCCGGA MslI 246 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 566 CATATGAATG RcaI 380 TCATGA T^CATGA 560 TCATGA SfaNI 200 GCATC GCATC(5/9) 500 GCATC SmlI 31 CTCAAG C^T[CT][GA]AG 697 CTCAAG StyI 455 CCTTGG C^C[AT][AT]GG 852 CCTTGG TseI 226 GCTGC G^C[AT]GC 343 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TfiI Tsp45I TspRI XhoII