(Circular) (Six-base) MAPSORT of: pFN21AE6243.seq Check: 2417 from: 1 to: 5608 pFN21AE6243 5608 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2456 2929 2456 Size: 473 5135 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 3671 5055 5323 1632 Size: 786 1253 1384 268 1917 Fragments arranged by size: 1917 1384 1253 786 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 3351 4923 3351 Size: 1572 4036 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2917 3143 3295 5404 1493 Size: 1424 226 152 2109 1697 Fragments arranged by size: 2109 1697 1424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2933 1870 Size: 73 990 4545 Fragments arranged by size: 4545 990 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2440 2473 2440 Size: 33 5575 BamHI G'GATC_C Cuts at: 2496 2938 3305 2496 Size: 442 367 4799 Fragments arranged by size: 4799 442 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2456 2929 4384 618 Size: 325 217 627 669 473 1455 1842 Fragments arranged by size: 1842 1455 669 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 2625 2927 1391 Size: 1234 302 4072 Fragments arranged by size: 4072 1234 302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2192 928 Size: 971 293 4344 Fragments arranged by size: 4344 971 293 BbvCI CC'TCA_GC Cuts at: 2762 2762 Size: 5608 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3799 3833 1903 Size: 34 1862 34 3678 Fragments arranged by size: 3678 1862 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2846 4219 136 Size: 107 122 71 631 605 1174 1373 1525 Fragments arranged by size: 1525 1373 1174 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 2327 3025 2327 Size: 698 4910 Bme1580I G_kGCm'C Cuts at: 1790 2632 3669 4825 1790 Size: 842 1037 1156 2573 Fragments arranged by size: 2573 1156 1037 842 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2683 3339 4297 Size: 416 53 83 399 24 1266 656 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1266 958 656 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2415 4268 1239 Size: 1176 1853 2579 Fragments arranged by size: 2579 1853 1176 Bpu10I CC'TnA_GC Cuts at: 2762 2762 Size: 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2021 2687 3733 4598 4896 2021 Size: 666 1046 865 298 2733 Fragments arranged by size: 2733 1046 865 666 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2181 2599 4041 4439 4713 4860 5271 2181 Size: 418 1442 398 274 147 411 2518 Fragments arranged by size: 2518 1442 418 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2710 2740 1178 Size: 30 165 30 1307 30 4046 Fragments arranged by size: 4046 1307 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5608 BseYI C'CCAG_C Cuts at: 2337 2351 2545 2648 2836 4811 5307 2337 Size: 14 194 103 188 1975 496 2638 Fragments arranged by size: 2638 1975 496 194 188 103 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2706 1163 Size: 573 970 4065 Fragments arranged by size: 4065 970 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 2625 2703 3669 3754 4825 2328 Size: 297 78 966 85 1071 3111 Fragments arranged by size: 3111 1071 966 297 85 78 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2625 2632 2703 2927 3669 3754 Size: 399 538 297 7 71 224 742 85 Cuts at: 3754 4825 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 297 224 85 71 7 BspEI T'CCGG_A Cuts at: 2181 2599 5271 2181 Size: 418 2672 2518 Fragments arranged by size: 2672 2518 418 BspHI T'CATG_A Cuts at: 2385 2565 3497 5227 2385 Size: 180 932 1730 2766 Fragments arranged by size: 2766 1730 932 180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 2963 844 Size: 1208 911 3489 Fragments arranged by size: 3489 1208 911 BsrBI CCG'CTC Cuts at: 1394 1823 2035 3495 1394 Size: 429 212 1460 3507 Fragments arranged by size: 3507 1460 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 2655 4103 4277 65 Size: 1183 1407 1448 174 1396 Fragments arranged by size: 1448 1407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 4252 4439 1700 Size: 110 241 2201 187 2869 Fragments arranged by size: 2869 2201 241 187 110 BsrGI T'GTAC_A Cuts at: 96 2799 96 Size: 2703 2905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 2087 1184 Size: 903 4705 BstXI CCAn_nnnn'nTGG Cuts at: 2492 2492 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2496 2637 2664 2938 3305 3690 3707 5159 Size: 524 141 27 274 367 385 17 1452 Cuts at: 5159 5245 5257 5603 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 524 385 367 346 274 141 86 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4117 Fragments arranged by size: 4117 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2823 3124 3918 3938 2823 Size: 301 794 20 4493 Fragments arranged by size: 4493 794 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2118 2153 529 Size: 35 1554 35 3984 Fragments arranged by size: 3984 1554 35 35 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2049 3055 Size: 54 1425 50 275 18 177 42 1006 Cuts at: 3055 3944 5573 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1006 889 275 177 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3538 1353 Size: 2185 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2623 2623 Size: 5608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2418 3671 4268 5055 5323 1239 Size: 393 783 3 1253 597 787 268 1524 Fragments arranged by size: 1524 1253 787 783 597 393 268 3 EcoO109I rG'GnC_Cy Cuts at: 2166 2166 Size: 5608 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 4755 1678 Size: 3077 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2190 2222 2388 2420 2710 Size: 32 135 32 618 32 166 32 290 Cuts at: 2710 2742 4249 4281 4323 4355 1373 Size: 32 1507 32 42 32 2626 Fragments arranged by size: 2626 1507 618 290 166 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2460 2933 2460 Size: 473 5135 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2137 4722 4906 1305 Size: 832 2585 184 2007 Fragments arranged by size: 2585 2007 832 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2575 3566 3925 4084 518 Size: 648 1089 320 991 359 159 2042 Fragments arranged by size: 2042 1089 991 648 359 320 159 MspA1I CmG'CkG Cuts at: 1606 2351 3024 3701 4849 5094 1606 Size: 745 673 677 1148 245 2120 Fragments arranged by size: 2120 1148 745 677 673 245 NaeI GCC'GGC Cuts at: 2053 2053 Size: 5608 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4117 Fragments arranged by size: 4117 939 552 NdeI CA'TA_TG Cuts at: 387 2572 387 Size: 2185 3423 NgoMIV G'CCGG_C Cuts at: 2051 2051 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 2552 1108 Size: 1444 4164 PfoI T'CCnGG_A Cuts at: 2392 2775 2392 Size: 383 5225 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2166 2166 Size: 5608 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2792 2824 2792 Size: 32 5576 PstI C_TGCA'G Cuts at: 830 2121 2960 830 Size: 1291 839 3478 Fragments arranged by size: 3478 1291 839 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2351 2351 Size: 5608 SacI G_AGCT'C Cuts at: 2625 2625 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2117 2956 4091 4772 4963 826 Size: 219 1072 839 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 839 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 2702 3712 4613 4875 820 Size: 197 926 93 666 1010 901 262 1553 Fragments arranged by size: 1553 1010 926 901 666 262 197 93 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2004 2460 2857 3047 513 Size: 552 939 456 397 190 3074 Fragments arranged by size: 3074 939 552 456 397 190 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 2328 3635 3652 3805 3990 4435 Size: 1255 495 253 1307 17 153 185 445 Cuts at: 4435 325 Size: 1498 Fragments arranged by size: 1498 1307 1255 495 445 253 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 2448 2799 Size: 274 80 33 51 1443 180 291 351 Cuts at: 2799 3854 5566 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1443 1055 351 291 274 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 2102 1316 Size: 786 4822 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2902 2902 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI