Restriction map for
pFN21AE3642
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI StuI SwaI Tru9I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 933 GTCTAC GT^[AC][GT]AC
AcsI 718 GAATTC [GA]^AATT[CT]
AlwNI 340 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 29 CCTCAGG CC^T[ACGT]AGG
Aor51HI 893 AGCGCT AGC^GCT
ApaI 131 GGGCCC GGGCC^C
ApoI 718 GAATTC [GA]^AATT[CT]
AspHI 589 GTGCTC G[AT]GC[AT]^C
AspI 868 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 642 TGGCCA TGG^CCA
BamHI 209 GGATCC G^GATCC
BanI 241 GGCACC G^G[CT][GA]CC
BanII 131 GGGCCC G[GA]GC[CT]^C
BglI 28 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 620 AGATCT A^GATCT
BsaI 850 GAGACC GGTCTC(1/5)
BsiEI 582 CGGCCG CG[GA][CT]^CG
BsiHKAI 589 GTGCTC G[AT]GC[AT]^C
Bsp1407I 482 TGTACA T^GTACA
BsrBI 439 GAGCGG CCGCTC(-3/-3)
BsrGI 482 TGTACA T^GTACA
BstXI 461 CCAAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 29 CCTCAGG CC^T[ACGT]AGG
DraII 131 GGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 582 CGGCCG C^GGCCG
EarI 592 CTCTTC CTCTTC(1/4)
EclXI 582 CGGCCG C^GGCCG
Eco47III 893 AGCGCT AGC^GCT
Eco52I 582 CGGCCG C^GGCCG
Eco81I 29 CCTCAGG CC^T[ACGT]AGG
EcoO109I 131 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 718 GAATTC G^AATTC
EcoT14I 710 CCAAGG C^C[AT][AT]GG
HaeII 893 AGCGCT [GA]GCGC^[CT]
HgaI 779 GCGTC GACGC(5/10)
Ksp632I 592 CTCTTC CTCTTC(1/4)
MaeIII 614 GTGAC ^GT[ACGT]AC
MluNI 642 TGGCCA TGG^CCA
MscI 642 TGGCCA TGG^CCA
NruI 942 TCGCGA TCG^CGA
PflFI 868 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 339 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 370 CTGCAG CTGCA^G
SapI 591 GCTCTTC GCTCTTC(1/4)
SbfI 369 CCTGCAGG CCTGCA^GG
SfaNI 881 GCATC GCATC(5/9)
SmlI 157 CTCAAG C^T[CT][GA]AG
Sse8387I 369 CCTGCAGG CCTGCA^GG
SspBI 482 TGTACA T^GTACA
StyI 710 CCAAGG C^C[AT][AT]GG
Tsp45I 614 GTGAC ^GT[GC]AC
Tth111I 868 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 339 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 458 CCACCAAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 210 GATCC GGATC(4/5)
209 GGATC
AvaII 632 GGTCC G^G[AT]CC
867 GGACC
BspMI 13 ACCTGC ACCTGC(4/8)
533 ACCTGC
BsrFI 290 ACCGGC [GA]^CCGG[CT]
914 ACCGGC
BsrI 836 CCAGT ACTGG(1/-1)
930 CCAGT
BstYI 209 GGATCC [GA]^GATC[CT]
620 AGATCT
Cfr10I 290 ACCGGC [GA]^CCGG[CT]
914 ACCGGC
DpnII 210 GATC ^GATC
621 GATC
DsaI 467 CCGTGG C^C[GA][CT]GG
692 CCACGG
EcoNI 333 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
426 CCTAAAGGAGG
HapII 291 CCGG C^CGG
915 CCGG
HpaII 291 CCGG C^CGG
915 CCGG
MboI 210 GATC ^GATC
621 GATC
MflI 209 GGATCC [GA]^GATC[CT]
620 AGATCT
MslI 120 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
180 CATGGAGGTG
MspI 291 CCGG C^CGG
915 CCGG
NdeII 210 GATC ^GATC
621 GATC
PleI 385 GACTC GAGTC(4/5)
771 GACTC
Sau3AI 210 GATC ^GATC
621 GATC
SfcI 370 CTGCAG C^T[GA][CT]AG
897 CTACAG
TaqI 449 TCGA T^CGA
548 TCGA
TfiI 451 GAATC G^A[AT]TC
734 GAATC
TthHB8I 449 TCGA T^CGA
548 TCGA
XhoII 209 GGATCC [GA]^GATC[CT]
620 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MboII MnlI
MvaI MvnI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI TseI Tsp509I
TspRI